When electroporation is used for introducing DNA into mammalian cells

1.	When electroporation is used for introducing DNA into mammalian cells
A.	P, Q
B.	Q, R
C.	P, S
D.	Q, S
Answer» B. Q, R

Discussion

No Comment Found

Related MCQs

The molar extinction coefficients of Trp and Tyr at 280 nm are 5690 and 1280 M 1.cm 1, respectively. The polypeptide chain of yeast alcohol dehydrogenase (37 kDa) contains 5 Trp and 14 Tyr residues. The absorbance at 280 nm of a 0.32 mg.mL 1 solution of yeast alcohol dehydrogenase measured in a cuvette of 1 cm pathlength will be _________. (Assume that the molar extinction coefficient values for Trp and Tyr apply to these amino acids in the yeast alcohol dehydrogenase).
Match the entries in the Group I with the elution conditions in Group II. Group I P. Ion-exchange Q. Hydrophobic chromatography R. Gel filtration column chromatography S. Chromatofocusing Group II 1. Isocratic solvent chromatography 2. Ampholytes 3. Increasing gradient of salt 4. Decreasing gradient of polarity
A protein is to be purified using ion- exchange column chromatography. The relationship between HETP (Height Equivalent to Theoretical Plate) and the linear liquid velocity of mobile phase is given by :H = A + Bu + Cuwhere H is HETP (m) and u is linear liquid velocity of mobile phase (m.s 1). The values of A, B and C are 3 10 8 m2.s 1, 3 s and 6 10 5 m, respectively. The number of theoretical plates based on minimum HETP for a column of 66 cm length will be ___________.
Match each parameter in group 1 with the appropriate measuring device in group 2 Group 1 P. Pressure Q. Foam R. Turbidity S. Flow rate Group 2 1. Photometer 2. Rotameter 3. Diaphragm gauge 4. Rubber sheathed electrode
Match the items in Group I with Group II. Group I P. Circular dichroism Q. X-ray crystallography R. Freeze-drying S. Ultracentrifugation Group II 1. Concentration 2. Sedimentation coefficient 3. Secondary structure determination 4. Tertiary structure determination
Match the entries in Group I with the methods of sterilization in Group II. Group I P. Serum Q. Luria broth f ltration R. Polypropylene tubes S. Biological safety cabinets Group II 1. Autoclave 2. Membrane 3. UV irradiation 4. Gamma irradiation 5. Dry heat
Match the coefficients in group 1 with their corresponding downstream processing steps given in group 2 Group 1 P. Sedimentation coefficient Q. Partition coefficient R. Rejection coefficient S. Activity coefficient Group 2 1. Aqueous two phase extraction 2. Ultrafiltration 3. Dialysis 4. Centrifugation
Match the properties in Group 1 with the downstream operation in Group 2 : Group 1 Group 2 P. Size 1. Extraction Q. Density 2. Distillation R. Volatility 3. Filtration S. Solubility 4. Sedimentation
Cesium chloride density gradient centrifugation is commonly used for the separation of DNA molecules. The buoyant density, , of a double stranded Cs+ DNA is given by the equation = 1.66 + 0.098XG + C where XG + C denotes
When electroporation is used for introducing DNA into mammalian cells (P) a carrier for DNA is not required (Q) the lipid bilayer (membrane) interacts with an electric pulse to generate permeation sites (R) the viability of the cells becomes approximately zero percent (S) the first step involves absorption of DNA on the cell membrane

When electroporation is used for introducing DNA into mammalian cells

Discussion

No Comment Found

Related MCQs

Reply to Comment