MCQOPTIONS
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MCQOPTIONS
This section includes 23 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Thermococcus litoralis grows at a temperature upto 98 degrees. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 2. |
Taq polymerase incorporates which residue at 3’ end? |
| A. | G |
| B. | T |
| C. | A |
| D. | C |
| Answer» D. C | |
| 3. |
What is the half life cycle for Taq polymerase? |
| A. | 40 minutes |
| B. | 80 minutes |
| C. | 10 minutes |
| D. | 50 minutes |
| Answer» B. 80 minutes | |
| 4. |
Which of the following activity is not present in Taq polymerase? |
| A. | 5’-3’ polymerase |
| B. | 5’-3’ exonuclease |
| C. | 3’-5’ exonuclease |
| D. | Both 5’-3’ polymerase and 5’-3’ exonuclease |
| Answer» D. Both 5’-3’ polymerase and 5’-3’ exonuclease | |
| 5. |
Primers and polymerases are added again during the reaction because they get consumed as the reaction proceeds. |
| A. | True |
| B. | False |
| Answer» C. | |
| 6. |
These are steps taken in carrying out the PCR reaction:i) Attaching of primers by coolingii) Denaturation of strandsiii) DNA synthesisiv) HeatingWhich is the correct order?(Mentioned from starting to ending the reaction) |
| A. | i)-ii)-iii)-iv) |
| B. | ii)-i)-iii)-iv) |
| C. | iv)-iii)-ii)-i) |
| D. | iv)-ii)-i)-iii) |
| Answer» E. | |
| 7. |
A reaction mixture for PCR consists of ____________ |
| A. | heat unstable polymerase |
| B. | primers in a limited amount |
| C. | deoxynucleoside triphosphate (dNTPs) |
| D. | a region complementary to the sequence to be amplified |
| Answer» D. a region complementary to the sequence to be amplified | |
| 8. |
The process of amplification of specific DNA sequences by an enzymatic process is termed as ____________ |
| A. | amplification |
| B. | polymerase chain reaction (PCR) |
| C. | translation |
| D. | microarrays |
| Answer» B. polymerase chain reaction (PCR) | |
| 9. |
WHICH_OF_THE_FOLLOWING_ACTIVITY_IS_NOT_PRESENT_IN_TAQ_POLYMERASE??$ |
| A. | 5’-3’ polymerase |
| B. | 5’-3’ exonuclease |
| C. | 3’-5’ exonuclease |
| D. | Both 5’-3’ polymerase and 5’-3’ exonuclease |
| Answer» D. Both 5‚Äö√Ñ√∂‚àö√ë‚àö¬•-3‚Äö√Ñ√∂‚àö√ë‚àö¬• polymerase and 5‚Äö√Ñ√∂‚àö√ë‚àö¬•-3‚Äö√Ñ√∂‚àö√ë‚àö¬• exonuclease | |
| 10. |
What is the half life cycle for Taq polymerase?$ |
| A. | 40 minutes |
| B. | 80 minutes |
| C. | 10 minutes |
| D. | 50 minutes |
| Answer» B. 80 minutes | |
| 11. |
Choose_the_correct_statement.$ |
| A. | Taq polymerase is having high processivity |
| B. | Processivity is defined in this case as synthesis of DNA by polymerase |
| C. | It requires a 5’ end for the elongation to take place |
| D. | The maximum size of the molecules which can be synthesized is 10kbp |
| Answer» C. It requires a 5‚Äö√Ñ√∂‚àö√ë‚àö¬• end for the elongation to take place | |
| 12. |
Thermococcus litoralis grows at a temperature upto 98 degrees. The given statement is true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 13. |
Polymerases are available with proof reading activity. Which of the following are the characteristics of these types of polymerases? |
| A. | They add a A residue at 3’ end |
| B. | They are obtained from Thermococcus litoralis |
| C. | They can’t be obtained from archaebacteria |
| D. | The marine bacteria from which they are obtained grow at temperatures lower than that of Thermus aquatics |
| Answer» C. They can‚Äö√Ñ√∂‚àö√ë‚àö¬•t be obtained from archaebacteria | |
| 14. |
Polymerases are also available from other Thermus species. Which of the following is correct? |
| A. | Thermus flavus gives Tfl enzyme |
| B. | Thermus thermopilus gives Tfl enzyme |
| C. | They are having proof reading activity |
| D. | Thermus flavus gives Tth enzyme |
| Answer» B. Thermus thermopilus gives Tfl enzyme | |
| 15. |
Taq_polymerase_incorporates_which_residue_at_3’_end?$ |
| A. | G |
| B. | T |
| C. | A |
| D. | C |
| Answer» D. C | |
| 16. |
All the molecules generated during PCR will not be full length. Some will also be of intermediate length. Which of the statements is correct? |
| A. | After first cycle, majority of the molecules will be full length and only some will be of intermediate length |
| B. | In the next cycle, each intermediate molecule will generate one intermediate molecule and one target molecule |
| C. | The number of full length molecules increase as number of cycles proceed |
| D. | The number of intermediate molecules increase geometrically and the number of target molecules increase arithmetically |
| Answer» C. The number of full length molecules increase as number of cycles proceed | |
| 17. |
Primers and polymerases are added again during the reaction, because they get consumed as the reaction proceeds. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» C. | |
| 18. |
Which of the following is not a condition for PCR? |
| A. | Initial melting carried out for 5 minutes at 94 degrees |
| B. | Initial melting followed by 30 cycles each consisting of melting for 1 minute at 94 degrees |
| C. | Renaturation for 5 minutes at 60 degrees |
| D. | DNA synthesis at 72 degrees for 1.5 minutes |
| Answer» D. DNA synthesis at 72 degrees for 1.5 minutes | |
| 19. |
These are steps taken in carrying out the PCR reaction: |
| A. | Attaching of primers by cooling |
| B. | Denaturation of strands |
| C. | DNA synthesis |
| D. | Heating |
| Answer» E. | |
| 20. |
Which of the following is a characteristic of Taq polymerase? |
| A. | It is a RNA polymerase |
| B. | It is heat stable |
| C. | It is obtained from thermophilic bacterium and can be grown in laboratory below a temperature of 75 degrees |
| D. | It is used in cellular synthesis processes and the optimum temperature is atleast 90 degrees |
| Answer» C. It is obtained from thermophilic bacterium and can be grown in laboratory below a temperature of 75 degrees | |
| 21. |
A reaction mixture for PCR consists of: |
| A. | heat unstable polymerase |
| B. | primers in limited amount |
| C. | deoxynucleoside triphosphate (dNTPs) |
| D. | a region complementary to the sequence to be amplified |
| Answer» D. a region complementary to the sequence to be amplified | |
| 22. |
What are primers? |
| A. | Primers are the short sequences at the end of the nucleotide sequences which are used for amplification |
| B. | Primers are the short sequences which are complementary to the nucleotides at the end of the sequence which is to be amplified |
| C. | Primers are the short sequences present anywhere in the nucleotide sequence to be amplified |
| D. | Primers are the short sequences which are complementary to the nucleotides anywhere in the sequence to be amplified |
| Answer» C. Primers are the short sequences present anywhere in the nucleotide sequence to be amplified | |
| 23. |
The process of amplification of specific DNA sequences by an enzymatic process is termed as: |
| A. | amplification |
| B. | polymerase chain reaction (PCR) |
| C. | translation |
| D. | microarrays |
| Answer» B. polymerase chain reaction (PCR) | |