Which of the following is not a condition for PCR? – McqOptions

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1.	Which of the following is not a condition for PCR?
A.	Initial melting carried out for 5 minutes at 94 degrees
B.	Initial melting followed by 30 cycles each consisting of melting for 1 minute at 94 degrees
C.	Renaturation for 5 minutes at 60 degrees
D.	DNA synthesis at 72 degrees for 1.5 minutes
Answer» D. DNA synthesis at 72 degrees for 1.5 minutes

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