MCQOPTIONS
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MCQOPTIONS
This section includes 23 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Selection of molecules by lack of inserts on the basis of the sacB gene is known as: |
| A. | positive selection |
| B. | negative selection |
| C. | counter selection |
| D. | sacB selection |
| Answer» D. sacB selection | |
| 2. |
How many origins of replication are there once the cyclization is carried out by loxP sites? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 3. |
Circularization must take place in the bacterial cells before the replication starts. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 4. |
What happens after packaging is carried out in the phage head? |
| A. | A sequence dependent cleavage of DNA is carried out |
| B. | A sequence independent cleavage of DNA is carried out |
| C. | They are linearized before they are infected into the bacterial cells |
| D. | They are circularized before they are infected into the bacterial cells |
| Answer» C. They are linearized before they are infected into the bacterial cells | |
| 5. |
Which of the following is not included as a step in cloning in bacteriophage PI? |
| A. | The growth of vector is done as a plasmid by using pBR322 origin of replication |
| B. | Cleaving with BamH1 is done and then products cleaved with Sau3A are ligated |
| C. | Incubation with mutant lysogen is done which doesn’t cleaves the recombinants at pac sites |
| D. | Incubation is done with mutant lysogen which doesn’t contains head and tail proteins |
| Answer» D. Incubation is done with mutant lysogen which doesn’t contains head and tail proteins | |
| 6. |
What is the function of the pac site in the bacteriophage PI? |
| A. | It is responsible for the initiation of packaging |
| B. | It is responsible for the termination of packaging |
| C. | It is responsible for the initiation of replication |
| D. | It is responsible for the termination of replication |
| Answer» B. It is responsible for the termination of packaging | |
| 7. |
If recombination takes place between elements in the second strain, it would replicate by the origin of replication of mini Mu. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 8. |
Choose the incorrect statement for ‘Mini Mu’? |
| A. | It is produced by cloning of bacteriophage Mu in vivo |
| B. | It contains reduced phage genome |
| C. | It doesn’t contain replication of origin of plasmid |
| D. | A selectable marker is added |
| Answer» D. A selectable marker is added | |
| 9. |
WHAT_HAPPENS_AFTER_PACKAGING_IS_CARRIED_OUT_IN_THE_PHAGE_HEAD??$ |
| A. | A sequence dependent cleavage of DNA is carried out |
| B. | A sequence independent cleavage of DNA is carried out |
| C. | They are linearized before they are infected into the bacterial cells |
| D. | They are circularized before they are infected into the bacterial cells |
| Answer» C. They are linearized before they are infected into the bacterial cells | |
| 10. |
WHICH_OF_THE_FOLLOWING_IS_NOT_INCLUDED_AS_A_STEP_IN_CLONING_IN_BACTERIOPHAGE_PI??$ |
| A. | The growth of vector is done as a plasmid by using pBR322 origin of replication |
| B. | Cleaving with BamH1 is done and then products cleaved with Sau3A are ligated |
| C. | Incubation with mutant lysogen is done which doesn’t cleaves the recombinants at pac sites |
| D. | Incubation is done with mutant lysogen which doesn’t contains head and tail proteins |
| Answer» D. Incubation is done with mutant lysogen which doesn‚Äö√Ñ√∂‚àö√ë‚àö¬•t contains head and tail proteins | |
| 11. |
How many origin of replication are there once the cyclization is carried out by loxP sites?$ |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 12. |
Circularization must take place in the bacterial cells before the replication starts. The given statement is true or false?$ |
| A. | True |
| B. | False |
| Answer» B. False | |
| 13. |
Selection of molecules by lack of inserts on the basis of sacB gene is known as: |
| A. | positive selection |
| B. | negative selection |
| C. | counter selection |
| D. | sacB selection |
| Answer» D. sacB selection | |
| 14. |
Choose the correct statement in respect to sacB gene? |
| A. | The cloning site for PAC lies on the side of sacB gene |
| B. | It is responsible for producing an enzyme which is responsible for catalysing sucrose into glucose and fructose |
| C. | Expression of sacB in the presence of sucrose is beneficial |
| D. | Levan is non-toxic for E. coli |
| Answer» C. Expression of sacB in the presence of sucrose is beneficial | |
| 15. |
Choose the incorrect statement for PAC vectors. |
| A. | In these vectors, the phage packaging signals are removed but the two P1 origin of replication persist |
| B. | They resemble BAC vectors |
| C. | They accept an insert of upto 300 kbp |
| D. | Instability is observed |
| Answer» E. | |
| 16. |
What is the function of loxP sites? |
| A. | It is responsible for linearization of DNA in the host bacterium |
| B. | It is responsible for circularization of DNA in the host bacterium |
| C. | It is responsible for conversion of single stranded DNA into double stranded DNA |
| D. | It is responsible for conversion of double stranded DNA into single stranded DNA |
| Answer» C. It is responsible for conversion of single stranded DNA into double stranded DNA | |
| 17. |
What is the function of pac site in the bacteriophage PI? |
| A. | It is responsible for initiation of packaging |
| B. | It is responsible for termination of packaging |
| C. | It is responsible for initiation of replication |
| D. | It is responsible for termination of replication |
| Answer» B. It is responsible for termination of packaging | |
| 18. |
What is the size of the insert that can be accommodated in the head of bacteriophage PI? |
| A. | 30-40 kbp |
| B. | 80-95 kbp |
| C. | 110-115 kbp |
| D. | 200-300 kbp |
| Answer» D. 200-300 kbp | |
| 19. |
Which of the following is a restriction for cloning in bacteriophage Mu? |
| A. | It is limited to handling only small number of phage particles |
| B. | It relies on whether the DNA is flanked by two mini Mu genes or not |
| C. | Recombination between the elements in the second strain is not a restriction |
| D. | Fragments of any genomic DNA can be cloned irrespective of the fact whether or not the phage can replicate in it or not |
| Answer» C. Recombination between the elements in the second strain is not a restriction | |
| 20. |
If recombination takes place between elements in the second strain, it would replicate by origin of replication of mini Mu. Is the given statement true or false. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 21. |
Which of the following is not included as a step for cloning mini Mu in vivo? |
| A. | A culture of target strain, whose DNA we want to clone is infected with mini Mu |
| B. | The phage is inserted at multiple sites in the genome |
| C. | Some of the packaged strain is flanked by two mini Mu genes |
| D. | The packaged phage is introduced into a second strain and Mu would replicate as a phage |
| Answer» E. | |
| 22. |
Choose the incorrect statement for ‘Mini Mu’?$ |
| A. | It is produced by cloning of bacteriophage Mu in vivo |
| B. | It contains reduced phage genome |
| C. | It doesn’t contains replication of origin of plasmid |
| D. | A selectable marker is added |
| Answer» D. A selectable marker is added | |
| 23. |
Bacteriophage Mu is also a phage, choose the correct statement for it. |
| A. | It is an example of temperate phage |
| B. | It is packaged into heads containing 50 kbp of DNA |
| C. | When the phage replicates it goes transposition only a few times |
| D. | Mu can’t be used for cloning in vivo |
| Answer» B. It is packaged into heads containing 50 kbp of DNA | |