WHICH_OF_THE_FOLLOWING_IS_NOT_INCLUDED_AS_A_STEP_IN_CLONING_IN_BACTERIOPHAGE_PI??$

Question

TuteeHUB · Accepted Answer

Incubation is done with mutant lysogen which doesn‚Äö√Ñ√∂‚àö√ë‚àö¬•t contains head and tail proteins

TuteeHUB · Answer

The growth of vector is done as a plasmid by using pBR322 origin of replication

TuteeHUB · Answer

Cleaving with BamH1 is done and then products cleaved with Sau3A are ligated

TuteeHUB · Answer

Incubation with mutant lysogen is done which doesn‚Äö√Ñ√∂‚àö√ë‚àö¬•t cleaves the recombinants at pac sites

1.	WHICH_OF_THE_FOLLOWING_IS_NOT_INCLUDED_AS_A_STEP_IN_CLONING_IN_BACTERIOPHAGE_PI??$
A.	The growth of vector is done as a plasmid by using pBR322 origin of replication
B.	Cleaving with BamH1 is done and then products cleaved with Sau3A are ligated
C.	Incubation with mutant lysogen is done which doesn‚Äö√Ñ√∂‚àö√ë‚àö¬•t cleaves the recombinants at pac sites
D.	Incubation is done with mutant lysogen which doesn‚Äö√Ñ√∂‚àö√ë‚àö¬•t contains head and tail proteins
Answer» D. Incubation is done with mutant lysogen which doesn‚Äö√Ñ√∂‚àö√ë‚àö¬•t contains head and tail proteins

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