What is the problem associated with historical DNA samples?

They degrade during repeated cooling and heating cycles

They are less in amount thus amplification is difficult

Because the samples are very old, there can be contamination

They degrade during repeated cooling and heating cycles

As the samples are old, the standard sequences for comparison is not present

PCR amplification can be used for which type of samples?

Recent samples are preferred but can be applied to old samples also

Equally to both recent and old samples

Recent samples are preferred but can be applied to old samples also

The genetic relatedness between organisms can be identified by studying the band patterns when different PCR products are analysed electrophoreically. This method is called as ____________

restriction fragment length polymorphism (RFLP)

amplified fragment length polymorphism (AFLP)

random amplification of polymorphic DNA (RAPD)

Which of the statements don t hold true for the forensics and the amplification carried out?

In cases of bone fragments which contain less than 300 nucleotides conventional methods can t be applied as they involve southern blotting, restriction digestion etc

In the case of forensics, conventional methods such as southern blotting are used very effectively

In cases of bone fragments which contain less than 300 nucleotides conventional methods can t be applied as they involve southern blotting, restriction digestion etc

The poor condition of DNA also makes the PCR amplification difficult

Microsatellites composed of simply varying repeats of CA sequences are used

Sickle cell anaemia is a genetic disorder. Which of the following doesn t holds true for it?

The conventional approach took weeks for the whole analyses to be carried out

The mutation is in alpha globulin gene

The conventional approach took weeks for the whole analyses to be carried out

Explore topic-wise MCQs in Genetic Engineering.

This section includes 7 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.	What is the problem associated with historical DNA samples?
A.	They are less in amount thus amplification is difficult
B.	Because the samples are very old, there can be contamination
C.	They degrade during repeated cooling and heating cycles
D.	As the samples are old, the standard sequences for comparison is not present
Answer» C. They degrade during repeated cooling and heating cycles

Discussion

2.	PCR amplification can be used for which type of samples?
A.	Old samples only
B.	Recent samples only
C.	Equally to both recent and old samples
D.	Recent samples are preferred but can be applied to old samples also
Answer» D. Recent samples are preferred but can be applied to old samples also

Discussion

3.	PCR is useful in population genetics because at times it can be used to study genetics of bacteria that can t be cultured axenically.
A.	True
B.	False
Answer» B. False

Discussion

4.	The genetic relatedness between organisms can be identified by studying the band patterns when different PCR products are analysed electrophoreically. This method is called as ____________
A.	restriction fragment length polymorphism (RFLP)
B.	amplified fragment length polymorphism (AFLP)
C.	random amplification of polymorphic DNA (RAPD)
D.	polymorphism
Answer» D. polymorphism

Discussion

5.	Which of the statements don t hold true for the forensics and the amplification carried out?
A.	In the case of forensics, conventional methods such as southern blotting are used very effectively
B.	In cases of bone fragments which contain less than 300 nucleotides conventional methods can t be applied as they involve southern blotting, restriction digestion etc
C.	The poor condition of DNA also makes the PCR amplification difficult
D.	Microsatellites composed of simply varying repeats of CA sequences are used
Answer» B. In cases of bone fragments which contain less than 300 nucleotides conventional methods can t be applied as they involve southern blotting, restriction digestion etc

Discussion

6.	Sickle cell anaemia is a genetic disorder. Which of the following doesn t holds true for it?
A.	It can be analysed by PCR
B.	It destroys a restriction site
C.	The mutation is in alpha globulin gene
D.	The conventional approach took weeks for the whole analyses to be carried out
Answer» D. The conventional approach took weeks for the whole analyses to be carried out

Discussion

7.	Cycle sequencing is the DNA sequencing where very less amounts of template are utilised for carrying out the sequencing.
A.	True
B.	False
Answer» B. False

Discussion

Explore topic-wise MCQs in Genetic Engineering.

What is the problem associated with historical DNA samples?

PCR amplification can be used for which type of samples?

PCR is useful in population genetics because at times it can be used to study genetics of bacteria that can t be cultured axenically.

The genetic relatedness between organisms can be identified by studying the band patterns when different PCR products are analysed electrophoreically. This method is called as ____________

Which of the statements don t hold true for the forensics and the amplification carried out?

Sickle cell anaemia is a genetic disorder. Which of the following doesn t holds true for it?

Cycle sequencing is the DNA sequencing where very less amounts of template are utilised for carrying out the sequencing.