MCQOPTIONS
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MCQOPTIONS
This section includes 16 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
For a convenient transformation system, _____ can be used for gene silencing. |
| A. | antisense RNA |
| B. | transposon insertion |
| C. | either antisense RNA or transposon insertion |
| D. | transposon insertion followed by antisense RNA |
| Answer» B. transposon insertion | |
| 2. |
The RNA level ___________ the steady-state level of the corresponding protein directly and the post-translational modification of the protein ____________ |
| A. | reflects, can be determined |
| B. | reflects, can’t be determined |
| C. | doesn’t necessarily reflects, can be determined |
| D. | doesn’t necessarily reflects, can’t be determined |
| Answer» E. | |
| 3. |
If a mutation perturbs the structure, then stability and folding are not affected. |
| A. | True |
| B. | False |
| Answer» C. | |
| 4. |
For getting a large amount of proteins to crystallize, which of the following should be used as an expression system? |
| A. | Bacterial system |
| B. | Yeast systems |
| C. | Eukaryotic systems |
| D. | Both eukaryotic and bacterial systems can be used |
| Answer» E. | |
| 5. |
If a full proteomic analysis of growth medium is carried out and is combined with ____________ genome sequence, genes for other _____________ proteins are also obtained. |
| A. | partial, defensive |
| B. | partial, secreted |
| C. | complete, defensive |
| D. | complete, secreted |
| Answer» E. | |
| 6. |
The disadvantage in the approach based on using PCR is that there is no permanent record until some of the bacterial cells are preserved. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 7. |
THE_RNA_LEVEL________THE_STEADY-STATE_LEVEL_OF_THE_CORRESPONDING_PROTEIN_DIRECTLY_AND_THE_POST-TRANSLATIONAL_MODIFICATION_OF_THE_PROTEIN_______.?$ |
| A. | reflects, can be determined |
| B. | reflects, can’t be determined |
| C. | doesn’t necessarily reflects, can be determined |
| D. | doesn’t necessarily reflects, can’t be determined |
| Answer» E. | |
| 8. |
For_a_convenient_transformation_system,_______can_be_used_for_gene_silencing.$ |
| A. | antisense RNA |
| B. | transposon insertion |
| C. | either antisense RNA or transposon insertion |
| D. | transposon insertion followed by antisense RNA |
| Answer» B. transposon insertion | |
| 9. |
If a mutation perturbs the structure, then stability and folding is not affected. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» C. | |
| 10. |
For getting large amount of proteins to crystallize, which of the following should be used as an expression system? |
| A. | Bacterial system |
| B. | Yeast systems |
| C. | Eukaryotic systems |
| D. | Both eukaryotic and bacterial systems can be used |
| Answer» E. | |
| 11. |
If a putative protein sequence is cloned in an expression vector and the expressed protein is not showing protease activity, then which of the following is not correct? |
| A. | The protein is not protease |
| B. | The protein can be incorrectly folded which can block the protease activity |
| C. | There might be some other cofactor required for protease activity |
| D. | The most commonly used expression system is E.coli |
| Answer» B. The protein can be incorrectly folded which can block the protease activity | |
| 12. |
If a full proteomic analysis of growth medium is carried out and is combined with ______ genome sequence, genes for other _____ proteins are also obtained. |
| A. | partial, defensive |
| B. | partial, secreted |
| C. | complete, defensive |
| D. | complete, secreted |
| Answer» E. | |
| 13. |
How many approaches are there in order to clone the complete genome? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 14. |
The disadvantage in the approach based on using PCR is that there is no permanent record until some of the bacterial cells are preserved. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 15. |
Which of the following is not suitable if the PCR product is non-specific? |
| A. | Adjusting the concentration of magnesium ions |
| B. | Increasing annealing temperature |
| C. | Using touchdown PCR |
| D. | Using inverse PCR |
| Answer» E. | |
| 16. |
Amplification of specific region can be done by using primers for specific regions. If the PCR product is ______ and is in sufficient quantity, then sequence can be determined ________ |
| A. | non-specific, directly |
| B. | non-specific, indirectly or directly |
| C. | specific, directly |
| D. | specific, indirectly |
| Answer» D. specific, indirectly | |