Which of the given statements is incorrect about Clustering by single linkage?

• A. In First step, a group of related sequences found in the all-against-all proteome comparison is subjected to a multiple sequence alignment usually by CLUSTALW
• B. A neighbor-joining algorithm is rarely used in this method
• C. This procedure and the algorithms are the same as those used to make a phylogenetic tree by the distance methods
• D. A distance matrix that shows the number of amino acid changes between each pair of sequences is made