Which of the following is not included as a step in cloning in bacteriophage PI?

Question

TuteeHUB · Accepted Answer

Incubation is done with mutant lysogen which doesn’t contains head and tail proteins

TuteeHUB · Answer

The growth of vector is done as a plasmid by using pBR322 origin of replication

TuteeHUB · Answer

Cleaving with BamH1 is done and then products cleaved with Sau3A are ligated

TuteeHUB · Answer

Incubation with mutant lysogen is done which doesn’t cleaves the recombinants at pac sites

TuteeHUB · Answer

Incubation is done with mutant lysogen which doesn’t contains head and tail proteins

1.	Which of the following is not included as a step in cloning in bacteriophage PI?
A.	The growth of vector is done as a plasmid by using pBR322 origin of replication
B.	Cleaving with BamH1 is done and then products cleaved with Sau3A are ligated
C.	Incubation with mutant lysogen is done which doesn’t cleaves the recombinants at pac sites
D.	Incubation is done with mutant lysogen which doesn’t contains head and tail proteins
Answer» D. Incubation is done with mutant lysogen which doesn’t contains head and tail proteins

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