Which of the following is not a step involved in site directed mutagenesis using a hypothetical example?

Question

TuteeHUB · Accepted Answer

Primary structure of putative enzyme is proposed with an asparagine residue replacing the serine present in the native enzyme

TuteeHUB · Answer

Derive the primary structure of the enzyme from the DNA sequence

TuteeHUB · Answer

Identification of mutant DNA by hybridization with radioactively labelled oligonucleotides

TuteeHUB · Answer

Cleavage by Hind III

TuteeHUB · Answer

Primary structure of putative enzyme is proposed with an asparagine residue replacing the serine present in the native enzyme

1.	Which of the following is not a step involved in site directed mutagenesis using a hypothetical example?
A.	Derive the primary structure of the enzyme from the DNA sequence
B.	Identification of mutant DNA by hybridization with radioactively labelled oligonucleotides
C.	Cleavage by Hind III
D.	Primary structure of putative enzyme is proposed with an asparagine residue replacing the serine present in the native enzyme
Answer» D. Primary structure of putative enzyme is proposed with an asparagine residue replacing the serine present in the native enzyme

Discussion