1.

Which of the following is not a step in producing penicillin -G-amidase?

A. Cleavage by Hind III
B. Replacement of asparagine residue with serine present in the native enzyme
C. Cleaved fragments cloned into a cosmid vector
D. Penicillin-G-amidase gene transferred on to pBR322 plasmids
Answer» C. Cleaved fragments cloned into a cosmid vector


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