Which of the following cannot be related to multiple sequence alignment?

• A. Many conserved and functionally critical amino acid residues can be identified in a protein multiple alignment
• B. Multiple sequence alignment is also an essential prerequisite to carrying out phylogenetic analysis of sequence families and prediction of protein secondary and tertiary structures
• C. Multiple sequence alignment also has applications in designing degenerate polymerase chain reaction (PCR) primers based on multiple related sequences
• D. This method does not contribute much to degenerate polymerase chain reaction (PCR) primers creation