When a sequence is generated from ____ ends of a single clone, the distance between the two opposing fragments of a clone is fixed to ________ meaning that they are always separated by a distance defined by a _____ length (normally 1,000 to 9,000 bases).

• A. both, an uncertain range, clone
• B. one, an uncertain range, clone
• C. both, a certain range, clone
• D. both, a certain range, gene