If expression of the coding sequence is to be carried in the vitro, then which of the statement holds true?

Question

TuteeHUB · Accepted Answer

Non radiolabelled amino acids are used

TuteeHUB · Answer

The extract in which the host DNA constructs are incubated is capable of translation only

TuteeHUB · Answer

The extract in which the host DNA constructs are incubated is capable of transcription only

TuteeHUB · Answer

The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc.

1.	If expression of the coding sequence is to be carried in the vitro, then which of the statement holds true?
A.	The extract in which the host DNA constructs are incubated is capable of translation only
B.	The extract in which the host DNA constructs are incubated is capable of transcription only
C.	The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc.
D.	Non radiolabelled amino acids are used
Answer» D. Non radiolabelled amino acids are used

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