At times, a second enzyme is used while the central portion is removed and the stuffer fragment is placed there. Choose the statement which doesn’t holds for this process.

• A. It is a method which is used for avoiding the relegation of central portion before the stuffer fragment is attached over there
• B. In this method, the central portion is cut with another enzyme after being separated from the arms
• C. The arms are also cut with this second enzyme
• D. It is not necessary to remove the fragments generated