Alkaline Phosphatase is used at times and the vector is treated with it. Choose the incorrect statement.

It removes 5’ terminal phosphate group from nucleic acids

The 5’ phosphate group is required for the ligation to take place

Two phosphate bonds should be formed for the complete ligation to take place

The ligation between vector and insert won’t take place

Alkaline Phosphatase is used at times and the vector is treated with i

1.	Alkaline Phosphatase is used at times and the vector is treated with it. Choose the incorrect statement.
A.	It removes 5’ terminal phosphate group from nucleic acids
B.	The 5’ phosphate group is required for the ligation to take place
C.	Two phosphate bonds should be formed for the complete ligation to take place
D.	The ligation between vector and insert won’t take place
Answer» E.