MCQOPTIONS
Saved Bookmarks
MCQOPTIONS
This section includes 12 Mcqs, each offering curated multiple-choice questions to sharpen your Bioinformatics knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which of the following is incorrect regarding Differential In-Gel Electrophoresis? |
| A. | Proteins are mixed together before electrophoresis on a two-dimensional gel |
| B. | Differentially expressed proteins in both conditions can’t be visualized in the same gel |
| C. | In this, Differences in protein expression patterns can be detected in a similar way as in fluorescent-labeled DNA microarrays |
| D. | Proteins from experimental and control samples are labeled with differently colored fluorescent dyes |
| Answer» C. In this, Differences in protein expression patterns can be detected in a similar way as in fluorescent-labeled DNA microarrays | |
| 2. |
Which of the following is incorrect regarding Mascot and ProFound? |
| A. | ProFound is a web server with a set of interconnected programs |
| B. | ProFound searches a protein sequence database using MS fingerprinting information |
| C. | Bayesian algorithm is not involved in ProFound |
| D. | Mascot is a web server that identifies proteins based on peptide mass fingerprints, sequence entries, or raw MS/MS data from one or more peptides |
| Answer» D. Mascot is a web server that identifies proteins based on peptide mass fingerprints, sequence entries, or raw MS/MS data from one or more peptides | |
| 3. |
ExPASY is a comprehensive proteomics web server with a suite of programs for searching peptide information from the SWISS-PROT and TrEMBL databases. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 4. |
WHICH_OF_THE_FOLLOWING_IS_INCORRECT_REGARDING_MASCOT_AND_PROFOUND??$ |
| A. | ProFound is a web server with a set of interconnected programs |
| B. | ProFound searches a protein sequence database using MS fingerprinting information |
| C. | Bayesian algorithm is not involved in ProFound |
| D. | Mascot is a web server that identifies proteins based on peptide mass fingerprints, sequence entries, or raw MS/MS data from one or more peptides |
| Answer» D. Mascot is a web server that identifies proteins based on peptide mass fingerprints, sequence entries, or raw MS/MS data from one or more peptides | |
| 5. |
Which of the following is incorrect regarding Differential In-Gel Electrophoresis?$ |
| A. | Proteins are mixed together before electrophoresis on a two-dimensional gel |
| B. | Differentially expressed proteins in both conditions can’t be visualized in the same gel |
| C. | In this, Differences in protein expression patterns can be detected in a similar way as in fluorescent-labeled DNA microarrays |
| D. | Proteins from experimental and control samples are labeled with differently colored fluorescent dyes |
| Answer» C. In this, Differences in protein expression patterns can be detected in a similar way as in fluorescent-labeled DNA microarrays | |
| 6. |
ExPASY is a comprehensive proteomics web server with a suite of programs for searching peptide information from the SWISS-PROT and TrEMBL databases? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 7. |
Which of the following is incorrect regarding the Protein Identification through Database Searching? |
| A. | MS characterization of proteins is highly dependent on bioinformatic analysis |
| B. | Bioinformatics programs can be used to search for the identity of a protein in a database of theoretically digested proteins |
| C. | Even in reality, the protease digestion is always perfect in MS |
| D. | The purpose of the database search is to find exact or nearly exact matches |
| Answer» D. The purpose of the database search is to find exact or nearly exact matches | |
| 8. |
Electrospray ionization MS and matrix-assisted laser desorption ionization (MALDI) MS only differ in the ionization procedure used. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 9. |
Which of the following is incorrect regarding Mass Spectrometry Protein Identification? |
| A. | The proteolysis doesn’t generate a pattern according to molecular weight |
| B. | Proteins can be identified and characterized using MS |
| C. | The proteins from a two dimensional gel system are first digested in situ with a protease |
| D. | Protein spots of interest are excised from the two-dimensional gel |
| Answer» B. Proteins can be identified and characterized using MS | |
| 10. |
Comparing two-dimensional gel images from various experiments can sometimes pose a challenge because the gels, unlike DNA microarrays, may shrink or warp. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 11. |
Which of the following is incorrect regarding 2D-Page? |
| A. | It stands for Two-dimensional polyacrylamide gel electrophoresis |
| B. | It separates proteins by charge only |
| C. | The gel is run in one direction in a pH gradient under a non-denaturing condition |
| D. | It works to separate proteins by isoelectric points (pI) |
| Answer» C. The gel is run in one direction in a pH gradient under a non-denaturing condition | |
| 12. |
The classic protein separation methods involve two-dimensional gel electrophoresis followed by gel image analysis. |
| A. | True |
| B. | False |
| Answer» B. False | |