Explore topic-wise MCQs in Testing Subject.

This section includes 657 Mcqs, each offering curated multiple-choice questions to sharpen your Testing Subject knowledge and support exam preparation. Choose a topic below to get started.

1.

Shadow casting is a technique of visualizing ___________________

A. isolated particles
B. mounts
C. shoot tips
D. root tips
Answer» B. mounts
2.

A protein is neutral at the isoelectric point.

A. True
B. False
C. May be True or False
D. Can't say
Answer» B. False
3.

Molecular beacons are short ____________________

A. polysaccarides
B. monosaccharides
C. oligonucleotides
D. phospholipids
Answer» D. phospholipids
4.

Which of the following enzymes in bacteria are responsible for restricting the growth of viruses?

A. restriction endonuclease
B. topoisomerase
C. gyrase
D. protease
Answer» B. topoisomerase
5.

Which technique is used to separate nucleic acids of size greater than 25 kb?

A. SDS-PAGE
B. Pulsed-field electrophoresis
C. 2D- gel electrophoresis
D. Isoelectric focusing
Answer» C. 2D- gel electrophoresis
6.

Image formation in electron microscope is based on ___________________________

A. column length
B. electron number
C. differential scattering
D. specimen size
Answer» D. specimen size
7.

Which type of gel is used for large nucleic acids?

A. acrylamide
B. cellulose
C. agarose
D. sephadex
Answer» D. sephadex
8.

Isotopes have the same number of protons.

A. True
B. False
C. May be True or False
D. Can't say
Answer» B. False
9.

MALDI is a technique of ______________________

A. ionization
B. fractionation
C. proteolysis
D. cell counting
Answer» B. fractionation
10.

Which of the following emitted particles consists of two protons?

A. alpha
B. beta
C. gamma
D. zeta
Answer» B. beta
11.

One Svedberg unit is equivalent to 10¯¹³ ______________

A. hours
B. minutes
C. seconds
D. milli-seconds
Answer» D. milli-seconds
12.

In TEM, the tissue is stained by floating on drops of ______________________

A. hydrocarbons
B. slow-molecular weight stains
C. heavy metal soutions
D. oil immersion
Answer» D. oil immersion
13.

Electrospray ionization is suited for proteins that are __________________

A. in matrix
B. in glycerol
C. in solution
D. of large size
Answer» D. of large size
14.

In SEM, the image is formed by the electrons that _________________

A. reflect back
B. ionize
C. undergo inversion
D. pass through
Answer» B. ionize
15.

The yeast two-hybrid system is used for studying ________________________

A. protein-protein interactions
B. pressure changes
C. molecular size
D. differentiation pattern
Answer» B. pressure changes
16.

Under favorable conditions, the protoplasts can grow into a ____________________

A. callus culture
B. organ culture
C. root culture
D. shoot culture
Answer» B. organ culture
17.

Which of the following molecules can be analyzed using a northern blot?

A. RNA
B. Carbohydrates
C. Proteins
D. DNA
Answer» B. Carbohydrates
18.

Trypsin is used for dissociating the tissue into single cells.

A. True
B. False
C. May be True or False
D. Can't say
Answer» B. False
19.

Liquid scintillation spectrometry is a method of detecting ________________________

A. X-rays
B. α-emitters
C. β-emitters
D. Gamma-rays
Answer» D. Gamma-rays
20.

The AFM uses a _______________ to scan the surface of a molecule.

A. X-ray film
B. Nanosized tip
C. Non-metal tip
D. Nanodrop
Answer» C. Non-metal tip
21.

Phenol is an active protein denaturant.

A. True
B. False
C. May be True or False
D. Can't say
Answer» B. False
22.

Which of the following is used as an ion-exchanger resin?

A. ethanol
B. cellulose
C. starch
D. collagen
Answer» C. starch
23.

The endonuclease HaeIII recognizes ______________________ sequence.

A. mono-nucleotide
B. bi-nucleotide
C. tri-nucleotide
D. tetra-nucleotide
Answer» D. tetra-nucleotide
24.

When was the nucleotide sequence of a viral genome first elucidated?

A. 1977
B. 1988
C. 1999
D. 2002
Answer» B. 1988
25.

In gel electrophoresis, the globular proteins move slower than the fibrous proteins.

A. True
B. False
C. May be True or False
D. Can't say
Answer» C. May be True or False
26.

The process of dedifferentiation in cell culture can give rise to ________________________

A. induced-pluripotent stem cells
B. carcinoma cells
C. single protoplasts
D. fused protoplasts
Answer» B. carcinoma cells
27.

Which of the following techniques delivers the amino-acid sequence of a peptide?

A. Tandem MS
B. GC-MS
C. LC-MS
D. SDS-PAGE
Answer» B. GC-MS
28.

In the secondary culture, cells are obtained from _______________________

A. primary culture
B. the organism
C. organ culture
D. phenotypic culture
Answer» B. the organism
29.

The identity and chemical properties of an atom are determined by ____________

A. critical temperature
B. critical freezing point
C. melting temperature
D. number of protons
Answer» E.
30.

In the liquid column chromatography, there are two phases namely ___________________ and ____________________

A. mobile, immobile
B. liquid, gel
C. viscous, non-viscous
D. flammable, inflammable
Answer» B. liquid, gel
31.

Which of the following is used to precipitate the nucleic acids?

A. glycerol
B. ethanol
C. methanol
D. ethylene
Answer» C. methanol
32.

Gel-filtration chromatography separates proteins based on their ___________________

A. pH
B. temperature
C. morphology
D. effective size
Answer» E.
33.

Bacterial artificial chromosomes are the __________________ of bacterial cells.

A. Polymerase enzyme
B. Proteases
C. F-factors
D. Exonucleases
Answer» D. Exonucleases
34.

In ion-exchange chromatography, proteins bound to the resin can be displaced by increasing the _____________________

A. strength of ionic buffer
B. size of sample
C. column volume
D. column width
Answer» B. size of sample
35.

Total nitrogen measurement can be used to measure ____________

A. pH drift
B. total protein
C. specific enzyme
D. viscosity
Answer» C. specific enzyme
36.

Polymerase chain reaction (PCR) was invented by ______________________

A. Kary Mullis
B. James Watson
C. John Hopkins
D. Hargobind Khorana
Answer» B. James Watson
37.

The scanning electron microscope is used to examine _____________

A. antigens
B. antibodies
C. anticoaggulants
D. cell surfaces
Answer» E.
38.

Which technique can be used to separate ¹⁵N and ¹⁴N?

A. Rate-zonal centrifugation
B. Isopycnic centrifugation
C. HPLC
D. Mass spectrometry
Answer» C. HPLC
39.

The temperature cycles in a polymerase chain reaction are in the order __________________

A. 95°, 60°, 72°
B. 60°, 72°, 95°
C. 72°, 60°, 95°
D. 95°, 72°, 60°
Answer» B. 60°, 72°, 95°
40.

cDNA libraries are produced from ______________________

A. ribonucleic acids
B. messenger RNAs
C. transfer RNAs
D. ribosomal RNAs
Answer» C. transfer RNAs
41.

The resolving power of TEM is derived from _______________

A. electrons
B. specimens
C. power
D. ocular system
Answer» B. specimens
42.

TEM and SEM are the same microscopy techniques.

A. True
B. False
C. May be True or False
D. Can't say
Answer» C. May be True or False
43.

The Feulgen stain is used for staining ____________________

A. plasma membrane
B. chromosomes
C. phospholipids
D. mitochondria
Answer» C. phospholipids
44.

During TEM, a vacuum is created inside the _________________________

A. room of operation
B. specimen
C. column
D. ocular system
Answer» D. ocular system
45.

The resolution attainable with standard TEM is less than the theoretical value.

A. True
B. False
C. May be True or False
D. Can't say
Answer» B. False
46.

The cathode of transmission electron microscope consists of a ____________________

A. tungsten wire
B. bulb
C. iron filament
D. gold wire
Answer» B. bulb
47.

Unstained specimens can be visualized using ________________

A. bright-field microscope
B. phase-contrast microscope
C. mass spectroscopy
D. spectrophotometer
Answer» C. mass spectroscopy
48.

Slides containing adherent paraffin sections are immersed in __________________

A. toluene
B. isoamyl alcohol
C. isopropanol
D. acetic acid
Answer» B. isoamyl alcohol
49.

Rhodamine is a ___________________

A. fluorochrome
B. antibody
C. stain
D. gel type
Answer» B. antibody
50.

Which of the following gives a three dimensional quality of the image?

A. Differential interference contrast
B. Bright-field microscope
C. Gas chromatography
D. Spectrophotometry
Answer» B. Bright-field microscope