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				This section includes 39 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.
| 1. | In transgenic plants of which of the following gene is transferred for the synthesis of PHB (polyhdroxybutyrate)? | 
| A. | Acetyl Co A reductase | 
| B. | Polyhydroxybutyrate synthease | 
| C. | Both (a) and (b) | 
| D. | Pyrophosphorylase | 
| Answer» D. Pyrophosphorylase | |
| 2. | Cultivated tomato species () is highly susceptible to | 
| A. | both (a) and (b) | 
| Answer» B. | |
| 3. | cDNA clone prepared to stigma mRNA of can be used as a probe on DNA digests of segregating populations to recognize | 
| A. | Aegilops | 
| B. | Triticum | 
| C. | Both (a) and (b) | 
| D. | S alleles | 
| Answer» E. | |
| 4. | Wheat variety Rendezvous, with resistance to eye spot () derived from , has a chloroplast genome derived from | 
| A. | Aegilops | 
| B. | Triticum | 
| C. | Both (a) and (b) | 
| D. | S alleles | 
| Answer» B. Triticum | |
| 5. | Which of the following govern sporophytic self-incompatibility in ? | 
| A. | Aegilops | 
| B. | Triticum | 
| C. | Both (a) and (b) | 
| D. | S alleles | 
| Answer» E. | |
| 6. | Cultivated tomato species () has a high degree of resistance towards | 
| A. | both (a) and (b) | 
| Answer» C. | |
| 7. | Which of the following constituent/(s) is/are added to help in speedy generation of meristem culture in case of improvement? | 
| A. | Auxins | 
| B. | Cytokinins | 
| C. | Both (a) and (b) | 
| D. | Adenine | 
| Answer» D. Adenine | |
| 8. | Which of the following mutant/(s) has/have increased amount of lysine and/or methionine in corn seeds? | 
| A. | Opaque-2 | 
| B. | Sugary-1 | 
| C. | Flory-2 | 
| D. | All of these | 
| Answer» E. | |
| 9. | When the transposon jumps into an otherwise functional gene, it has the effect of | 
| A. | blocking correct transcription | 
| B. | initiating correct transcription | 
| C. | propagating correct transcription | 
| D. | initiating some genetic codes | 
| Answer» B. initiating correct transcription | |
| 10. | Cultivated tomato species (Lycopersicon esculentum) has a high degree of resistance towards | 
| A. | <i>Meloidogyne spp.nematodes</i> | 
| B. | <i>L. peruviaanum</i> | 
| C. | both (a) and (b) | 
| D. | <i>Solarium melongena</i> | 
| Answer» C. both (a) and (b) | |
| 11. | Which of the following constituent/(s) is/are added to help in speedy generation of meristem culture in case of Ipomoea batatas improvement? | 
| A. | Auxins | 
| B. | Cytokinins | 
| C. | Both (a) and (b) | 
| D. | Adenine | 
| Answer» D. Adenine | |
| 12. | cDNA clone prepared to stigma mRNA of Brassica oleracea can be used as a probe on DNA digests of segregating populations to recognize | 
| A. | Aegilops | 
| B. | Triticum | 
| C. | Both (a) and (b) | 
| D. | S alleles | 
| Answer» E. | |
| 13. | Cytoplast (somatic cell hybridization) is the process in which | 
| A. | two protoplast along with their nuclei fuse | 
| B. | a normal protoplast along with an enucleated protoplast fuse | 
| C. | both (a) and (b) | 
| D. | two enucleated protoplasts fuse | 
| Answer» D. two enucleated protoplasts fuse | |
| 14. | Which of the following is the class of hybridization? | 
| A. | Intervarietal | 
| B. | Interspecific | 
| C. | Intergeneric | 
| D. | all of these | 
| Answer» E. | |
| 15. | For increased level of mannitol in transgenic tobacco plants which of the following gene is transferred? | 
| A. | Mannitol dehydrogenase | 
| B. | Mannitol synthease | 
| C. | Both (a) and (b) | 
| D. | Mannitol pyrophosphorylase | 
| Answer» B. Mannitol synthease | |
| 16. | Cultivated tomato species (Lycopersicon esculentum) is highly susceptible to | 
| A. | <i>Meloidogyne spp. nematodes</i> | 
| B. | <i>L. peruviaanum</i> | 
| C. | both (a) and (b) | 
| D. | <i>Solarium melongena</i> | 
| Answer» B. <i>L. peruviaanum</i> | |
| 17. | Microinjection technique of introducing NA is a | 
| A. | physical method | 
| B. | biological method | 
| C. | combination of physical and biological method | 
| D. | chemical method | 
| Answer» B. biological method | |
| 18. | Protoplast fusion technique generally involves | 
| A. | isolation only | 
| B. | isolation and fusion | 
| C. | isolation, fusion and culturing | 
| D. | hybridization between two species | 
| Answer» D. hybridization between two species | |
| 19. | Which of the following technique can be used to develop nematode resistant plants? | 
| A. | Embryo-rescue | 
| B. | Mutation | 
| C. | Non-recombinant DNA | 
| D. | All of these | 
| Answer» E. | |
| 20. | Random amplified polymorphic DNA (RAPD) is a method that | 
| A. | stimulate production of sense RNA to compensate | 
| B. | activate the expression of all genes in a biochemical pathway | 
| C. | eliminate the expression of all genes in a biochemical pathway | 
| D. | reveals intra-specific variation and diversity between species | 
| Answer» E. | |
| 21. | Commercial sugarcane is heterozygons with germplasm from | 
| A. | 1-3 Saccharum species | 
| B. | 3-5 Saccharum species | 
| C. | 5-7 Saccharum species | 
| D. | 7-9 Saccharum species | 
| Answer» C. 5-7 Saccharum species | |
| 22. | Which of the following govern sporophytic self-incompatibility in Brassica? | 
| A. | Aegilops | 
| B. | Triticum | 
| C. | Both (a) and (b) | 
| D. | S alleles | 
| Answer» E. | |
| 23. | Somaclonal variation method refers to | 
| A. | heritable changes | 
| B. | genetic isolation | 
| C. | small number of species having limited commercial potential | 
| D. | large number of plant cells | 
| Answer» B. genetic isolation | |
| 24. | and cyclo dextrins are produced by | 
| A. | transgenic tomato | 
| B. | transgenic maize | 
| C. | transgenic potato | 
| D. | transgenic wheat | 
| Answer» D. transgenic wheat | |
| 25. | and cyclo dextrins are used for | 
| A. | pharmaceutical delivery system | 
| B. | flavour and odour enhancement in foods | 
| C. | removal of undesired compounds like caffeine from foods | 
| D. | all of the above | 
| Answer» E. | |
| 26. | Meristem culture of banana enables | 
| A. | rapid multiplication | 
| B. | elimination of bunch top virus disease | 
| C. | both (a) and (b) | 
| D. | slow growth | 
| Answer» D. slow growth | |
| 27. | Wheat variety Rendezvous, with resistance to eye spot (Pseudo-cercosporella herpotrichoides) derived from Aegilops ventricosa, has a chloroplast genome derived from | 
| A. | Aegilops | 
| B. | Triticum | 
| C. | Both (a) and (b) | 
| D. | S alleles | 
| Answer» B. Triticum | |
| 28. | The intervention in the communication system of nematodes offer several possibilities of bio control. Which of the following(s) play(s) a very important role in the host recognition? | 
| A. | Protein | 
| B. | Lipid | 
| C. | Carbohydrate moiety | 
| D. | Minerals | 
| Answer» D. Minerals | |
| 29. | Human serum albumin has been synthesized by | 
| A. | transgenic potato and tobacco plants | 
| B. | transgenic maize plants | 
| C. | transgenic wheat plants | 
| D. | any of the above | 
| Answer» B. transgenic maize plants | |
| 30. | The tumor phenotype, which can be maintained indefinitely in tissue culture, results from the expression of genes on the | 
| A. | T-DNA | 
| B. | c-DNA | 
| C. | r-DNA | 
| D. | m-RNA | 
| Answer» B. c-DNA | |
| 31. | Establishment of embryogenic cell suspensions with high regeneration potential is a pre-requisite for successful | 
| A. | protoplast culture | 
| B. | detection of nucleic acid sequence | 
| C. | restriction of fragment length polymorphisms | 
| D. | microinjection | 
| Answer» B. detection of nucleic acid sequence | |
| 32. | Which of the following transfers a set of genes encoded in a region called T-DNA of the Ti-plasmid into cells at wound positions? | 
| A. | <i>A. tumefaciens</i> | 
| B. | <i>L. peruvianum</i> | 
| C. | <i>L. birsutum</i> | 
| D. | <i>A. sterilis</i> | 
| Answer» B. <i>L. peruvianum</i> | |
| 33. | Human serum albumin in case of transgenic plant is secreted in | 
| A. | transgenic leaf tissues | 
| B. | transgenic root tissues | 
| C. | is not secreted out of plant | 
| D. | transgenic stem tissues | 
| Answer» B. transgenic root tissues | |
| 34. | Avermectins (AVM) are macrocyclic lactones which have potent antihelminthic and insecticidal action. They are generally derived from the mycelia of | 
| A. | <i>Streptomyces avermitilis</i> | 
| B. | <i>B. thrunigiensis</i> | 
| C. | <i>P. flourescens</i> | 
| D. | <i>L. peruvianum</i> | 
| Answer» B. <i>B. thrunigiensis</i> | |
| 35. | Plant tissue culture technique is used to select somaclones that are | 
| A. | resistant to herbicide | 
| B. | not resistant to herbicide | 
| C. | resistant to insecticides | 
| D. | not resistant to insecticides | 
| Answer» B. not resistant to herbicide | |
| 36. | Which of the following has been confirmed on sugarcane genotypes? | 
| A. | Bacilliform virus | 
| B. | Cytomegalovirus | 
| C. | Varicella-zoster virus | 
| D. | Erythrovirus | 
| Answer» B. Cytomegalovirus | |
| 37. | The hybrid embryo can be rescued from damage/collapse by growing it | 
| A. | at low temperature | 
| B. | at low pH | 
| C. | at low water activity | 
| D. | in artificial medium | 
| Answer» E. | |
| 38. | Involvement of sugarcane phytoalexins in red-hot resistance has been observed. The phytoalexin compound is reported to be | 
| A. | 3-deoxyanthocyanidin | 
| B. | luteolinidin | 
| C. | apigeninidin | 
| D. | 5-0 apigeninidin | 
| Answer» B. luteolinidin | |
| 39. | In transgenic plants of Arabidipsis thaliana which of the following gene is transferred for the synthesis of PHB (polyhdroxybutyrate)? | 
| A. | Acetyl Co A reductase | 
| B. | Polyhydroxybutyrate synthease | 
| C. | Both (a) and (b) | 
| D. | Pyrophosphorylase | 
| Answer» D. Pyrophosphorylase | |