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				This section includes 86 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.
| 1. | The spoilage most commonly occurring in wine is tourne which refers to | 
| A. | acid formation from aldehyde and ketones | 
| B. | acid formation from sugars, glucose and fructose | 
| C. | both (a) and (b) | 
| D. | none of the above | 
| Answer» C. both (a) and (b) | |
| 2. | If starch containing substrates are used for ethanol production, yeast strain can't be used directly because | 
| A. | it doesn't contain amylases to hydrolyze starch | 
| B. | starch is not a suitable substrate for the production of ethanol | 
| C. | it is converted to pentose sugar | 
| D. | none of the above | 
| Answer» B. starch is not a suitable substrate for the production of ethanol | |
| 3. | S cerevisiae start producing ethanol with in | 
| A. | 10 h | 
| B. | 12 h | 
| C. | 15 h | 
| D. | 24 h | 
| Answer» C. 15 h | |
| 4. | Which of the following is used to check vortex and to improve aeration efficiency in a fermentor? | 
| A. | Impeller | 
| B. | Baffles | 
| C. | Sparger | 
| D. | All of these | 
| Answer» C. Sparger | |
| 5. | How is air supply to the fermentor sterilized? | 
| A. | By heat filtration | 
| B. | Filtration through fibrous material | 
| C. | Filtration through granular material | 
| D. | All of the above | 
| Answer» E. | |
| 6. | The Zygomonas has a merit over yeast because | 
| A. | it has more osmotic tolerance to higher sugar concentration | 
| B. | it has high tolerance to ethanol | 
| C. | it has more specific growth rate | 
| D. | all of the above | 
| Answer» E. | |
| 7. | Which substrate is used in the fermentation of citric acid? | 
| A. | Beet molasses | 
| B. | Sucrose | 
| C. | Starch hydrolysate | 
| D. | All of these | 
| Answer» E. | |
| 8. | Which of the following microorganisms help in the ethanol production? | 
| A. | Saccharomyces cerevisiae | 
| B. | Zygomonas mobilis | 
| C. | Saccharomyces uvarum | 
| D. | All of these | 
| Answer» E. | |
| 9. | To maintain aseptic conditions during fermentation which of the following is needed? | 
| A. | Sterilization of fermentor | 
| B. | Sterilization of air supply | 
| C. | Aeration and agitation | 
| D. | All of these | 
| Answer» E. | |
| 10. | Which of the following nitrogen sources is added in the fermentation medium of citric acid production? | 
| A. | Ammonium salt | 
| B. | Urea | 
| C. | Both (a) and (b) | 
| D. | Peptone | 
| Answer» D. Peptone | |
| 11. | Malt vinegar is produced from | 
| A. | fruit juices | 
| B. | malted grain | 
| C. | ethanol | 
| D. | ale | 
| Answer» C. ethanol | |
| 12. | Vinegar production consists of | 
| A. | aerobic fermentation | 
| B. | anaerobic fermentation | 
| C. | aerobic fermentation followed by anaerobic fermentation | 
| D. | anaerobic fermentation followed by aerobic fermentation | 
| Answer» E. | |
| 13. | The crushed grapes are known as | 
| A. | malt | 
| B. | must | 
| C. | wort | 
| D. | sonti | 
| Answer» C. wort | |
| 14. | If a winemaker wanted to make a deeply colored red wine, which of the following should not be done? | 
| A. | Bleed off juice prior to fermenting on skins | 
| B. | Ferment at a high temperature | 
| C. | Use whole clusters | 
| D. | Punch down at least once a day | 
| Answer» D. Punch down at least once a day | |
| 15. | Alcohol content of sake varies from | 
| A. | 1-5 % | 
| B. | 4-17 % | 
| C. | 17-25% | 
| D. | more than 25% | 
| Answer» C. 17-25% | |
| 16. | If the malolactic fermentation is not carried out, | 
| A. | the wine will have an odd odor | 
| B. | malic acid may precipitate, leaving small crystals | 
| C. | the wine may be microbially unstable | 
| D. | the wine will be too low in acid | 
| Answer» D. the wine will be too low in acid | |
| 17. | Wine is obtained after alcoholic fermentation of | 
| A. | rice | 
| B. | wheat | 
| C. | grapes | 
| D. | barley | 
| Answer» D. barley | |
| 18. | In a hot climate, grapes lose acidity because | 
| A. | enough tartaric acid is not made | 
| B. | enough malic acid is not made | 
| C. | of break down of malic acid | 
| D. | of break down of tartaric acid | 
| Answer» D. of break down of tartaric acid | |
| 19. | How long does it take to complete fermentation process in case of white wine? | 
| A. | 1-3 days | 
| B. | 3-5 days | 
| C. | 5-7 days | 
| D. | 7-11 days | 
| Answer» E. | |
| 20. | Fining a wine is defined as | 
| A. | adding one substance to remove another | 
| B. | removing small particles | 
| C. | adding acid | 
| D. | removing tannin | 
| Answer» B. removing small particles | |
| 21. | Which of the following is true for the lager beer? | 
| A. | Bottom fermenting yeast is used for the preparation | 
| B. | Top yeast is used for the preparation | 
| C. | Require high fermentation temperature | 
| D. | Both (b) and (c) | 
| Answer» B. Top yeast is used for the preparation | |
| 22. | Wort is | 
| A. | an aqueous extract of malt | 
| B. | malted barley | 
| C. | coagulated protein obtained during boiling | 
| D. | none of the above | 
| Answer» B. malted barley | |
| 23. | How long does it take to complete fermentation process in case of red wine? | 
| A. | 1-3 days | 
| B. | 3-5 days | 
| C. | 5-7 days | 
| D. | 7-11 days | 
| Answer» C. 5-7 days | |
| 24. | Which of the following microorganism is used for the production of citric acid? | 
| A. | Lactobacillus bulgaricus | 
| B. | Saccharomyces cerevisiae | 
| C. | Aspergillus niger | 
| D. | Streptococcus lactis | 
| Answer» D. Streptococcus lactis | |
| 25. | In koji process for the fermentation to produce citric acid, | 
| A. | A. niger is used with solid substrate as sweet potato | 
| B. | A. niger floats on the surface of solution | 
| C. | Fungal mycelium grows through out the solution in a deep tank | 
| D. | both (a) and (b) | 
| Answer» B. A. niger floats on the surface of solution | |
| 26. | Which of the following organism is not used in any of the traditional industrial fermentation? | 
| A. | E. coli | 
| B. | Yeast | 
| C. | Bacillus | 
| D. | Pseudomonas | 
| Answer» B. Yeast | |
| 27. | Carbonic maceration wines | 
| A. | are made from crushed grapes | 
| B. | are very fruity white wines | 
| C. | are produced in California | 
| D. | are low tannin red wines | 
| Answer» E. | |
| 28. | In submerged process for the fermentation to produce citric acid, | 
| A. | A. niger is used with solid substrate as sweet potato | 
| B. | A. niger floats on the surface of solution | 
| C. | Fungal mycelium grows through out the solution in a deep tank | 
| D. | both (a) and (b) | 
| Answer» D. both (a) and (b) | |
| 29. | Yeast is used in the production of | 
| A. | ethyl alcohol | 
| B. | acetic acid | 
| C. | cheese | 
| D. | curd | 
| Answer» B. acetic acid | |
| 30. | Chaptalization is | 
| A. | adding sucrose to must | 
| B. | adding grape juice to must | 
| C. | adding sugar to wine before bottling | 
| D. | adding grape juice to wine before bottling | 
| Answer» B. adding grape juice to must | |
| 31. | A hydrometer | 
| A. | measures wine acid | 
| B. | requires a few drops of juice | 
| C. | measures alcohol in the wine | 
| D. | measures grape sugar | 
| Answer» E. | |
| 32. | High ethanol concentration | 
| A. | promotes yeast growth | 
| B. | inhibits yeast growth | 
| C. | promotes bacterial growth | 
| D. | inhibits bacterial growth | 
| Answer» C. promotes bacterial growth | |
| 33. | Malting process allows malt amylase and proteinases to degrade starch and protein to | 
| A. | glucose and peptone as well as peptides | 
| B. | glucose and amino acids | 
| C. | maltose and peptone as well as peptides | 
| D. | maltose and amino acids | 
| Answer» D. maltose and amino acids | |
| 34. | Punching down is done | 
| A. | for the same reasons as pumping over | 
| B. | to extract tannin | 
| C. | both (a) and (b) | 
| D. | to keep the cap dry | 
| Answer» E. | |
| 35. | In sparkling wine production, the cuvee is | 
| A. | the wire cage that holds the cork in place | 
| B. | the sugar addition just before corking | 
| C. | the sugar/yeast addition to the base wine | 
| D. | the base wine | 
| Answer» E. | |
| 36. | Volatile acidity is | 
| A. | also known as total acidity | 
| B. | produced by acetobacter | 
| C. | produced by Brettanomyces | 
| D. | known for producing a mousy or barnyard aroma | 
| Answer» C. produced by Brettanomyces | |
| 37. | Wort is boiled with the hops | 
| A. | to help in protein coagulation | 
| B. | to provide bitter taste and flavour to the beer | 
| C. | to help in the coagulation of tannins | 
| D. | All of the above | 
| Answer» C. to help in the coagulation of tannins | |
| 38. | As the fermentation for the ethanol production is over, the cells are separated to get the biomass of yeast cells that is used | 
| A. | for next fermentation | 
| B. | as single cell protein for animal feed | 
| C. | as manure | 
| D. | all of the above | 
| Answer» C. as manure | |
| 39. | Alegar is a type of vinegar produced from | 
| A. | fruit juices | 
| B. | malted grain | 
| C. | ethanol | 
| D. | ale | 
| Answer» E. | |
| 40. | After storage and recarbonation, left over yeast is separated by | 
| A. | centrifugation | 
| B. | filtration | 
| C. | cell disruption | 
| D. | need not to be separated | 
| Answer» C. cell disruption | |
| 41. | Spirit vinegar is produced from | 
| A. | fruit juices | 
| B. | malted grain | 
| C. | ethanol | 
| D. | ale | 
| Answer» D. ale | |
| 42. | Which of the following is used for agitation in a fermentor? | 
| A. | Impeller | 
| B. | Baffles | 
| C. | Sparger | 
| D. | All of these | 
| Answer» B. Baffles | |
| 43. | What is the pH of the medium when sucrose is used as substrate for the production of citric acid? | 
| A. | 3 | 
| B. | 4 | 
| C. | 5 | 
| D. | 6 | 
| Answer» B. 4 | |
| 44. | Ales are the beer in which fermentation is carried out using | 
| A. | top yeast | 
| B. | bottom yeast | 
| C. | either of the above | 
| D. | middle yeast | 
| Answer» B. bottom yeast | |
| 45. | Which is not correct about blush wines? | 
| A. | They are stored in barrels | 
| B. | They are bottled within a year | 
| C. | They are usually slightly sweet | 
| D. | They are fermented in stainless steel | 
| Answer» B. They are bottled within a year | |
| 46. | Lagers are the beer in which fermentation is carried out using | 
| A. | top yeast | 
| B. | bottom yeast | 
| C. | either of these | 
| D. | middle yeast | 
| Answer» C. either of these | |
| 47. | Cider vinegar is produced from | 
| A. | fruit juices | 
| B. | malted grain | 
| C. | ethanol | 
| D. | ale | 
| Answer» B. malted grain | |
| 48. | Ethanol is produced by | 
| A. | continuous fermentation | 
| B. | batch fermentation | 
| C. | both (a) and (b) | 
| D. | none of these | 
| Answer» D. none of these | |
| 49. | The term Racking refers to | 
| A. | stacking unlabeled wine in bins for aging | 
| B. | adding yeast to initiate fermentation | 
| C. | storing hoses to drain on a slanted board | 
| D. | removing clear liquid from sediment | 
| Answer» E. | |
| 50. | During recovery step of citric acid, calcium citrate formed is treated with which of the following acid to precipitate calcium? | 
| A. | HCl | 
| B. | HNO3 | 
| C. | H2SO4 | 
| D. | None of these | 
| Answer» D. None of these | |