MCQOPTIONS
Saved Bookmarks
MCQOPTIONS
This section includes 151 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Plant transformation vectors based on can generally be divided into |
| A. | two vectors |
| B. | four vectors |
| C. | six vectors |
| D. | eight vectors |
| Answer» B. four vectors | |
| 2. |
The size of the virulent plasmid of is |
| A. | 40-80 kb |
| B. | 80-120 kb |
| C. | 140-235 kb |
| D. | >235 kb |
| Answer» D. >235 kb | |
| 3. |
Virulence trait of is borne on |
| A. | chromosomal DNA |
| B. | tumour inducing plasmid DNA |
| C. | both chromosomal and plasmid DNA |
| D. | cryptic plasmid DNA |
| Answer» C. both chromosomal and plasmid DNA | |
| 4. |
is a |
| A. | gram (+) bacteria |
| B. | gram (-) bacteria |
| C. | a fungi |
| D. | a yeast |
| Answer» C. a fungi | |
| 5. |
The transfer of intermediate vectors into are brought about by |
| A. | transformation |
| B. | biparental mating |
| C. | triparental mating |
| D. | transduction |
| Answer» D. transduction | |
| 6. |
In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by |
| A. | A |
| B. | B |
| C. | C |
| D. | D |
| Answer» E. | |
| 7. |
Which of the following are used as selection marker for the cells transformed with ? |
| A. | Neomycin phosphotransferase |
| B. | Streptomycin phosphotransferase |
| C. | Hygromycin phosphotransferase |
| D. | any of the above |
| Answer» E. | |
| 8. |
The direct repeats flanking the T-DNA of are known as |
| A. | cos site |
| B. | flanking sequences |
| C. | border sequences |
| D. | transfer sequences |
| Answer» D. transfer sequences | |
| 9. |
Phosphinothricin acetyl transferase is encoded by |
| A. | gene bxn in Klebsiella pneumoniae |
| B. | bar gene in Streptomyces spp |
| C. | both (a) and (b) |
| D. | none of these |
| Answer» C. both (a) and (b) | |
| 10. |
A __________ is a small laboratory set up to simulate conditions of a particular environment. |
| A. | microcosm |
| B. | mibridization |
| C. | bioremediation |
| D. | rhizosecretion |
| Answer» B. mibridization | |
| 11. |
Insect resistance in the transgenic plant has been achieved by |
| A. | transferring genes for Bt toxins |
| B. | transferring genes for protease inhibitors |
| C. | transferring genes for other insecticidal secondary metabolities |
| D. | all of the above |
| Answer» E. | |
| 12. |
Which of the following compounds has been produced in transgenic plants to improve tolerance to salt stress and water deficit? |
| A. | Sucrose |
| B. | Mannitol |
| C. | Nicotine |
| D. | Octopine |
| Answer» C. Nicotine | |
| 13. |
Which of the following metabolites are implicated in stress tolerance? |
| A. | Proline |
| B. | Betaines |
| C. | Both (a) and (b) |
| D. | Citrate |
| Answer» D. Citrate | |
| 14. |
Wheat variety Rendezvous, with resistance to eye spot (Pseudo-cercosporella herpotrichoides) derived from Aegilops ventricosa, has a chloroplast genome derived from |
| A. | Aegilops |
| B. | Triticum |
| C. | Both (a) and (b) |
| D. | S alleles |
| Answer» B. Triticum | |
| 15. |
Which of the following has been confirmed on sugarcane genotypes? |
| A. | Bacilliform virus |
| B. | Cytomegalovirus |
| C. | Varicella-zoster virus |
| D. | Erythrovirus |
| Answer» B. Cytomegalovirus | |
| 16. |
Recalcitrant seeds are |
| A. | resistant to drying and freezing temperature |
| B. | killed by drying and freezing temperature |
| C. | both (a) and (b) |
| D. | none of above |
| Answer» C. both (a) and (b) | |
| 17. |
Bruise resistant tomatoes have been developed by the expression of antisense RNA against |
| A. | glycerol 1 phosphate acyl transferase |
| B. | polygalactouranase |
| C. | ACC deaminase |
| D. | Sucrose phosphate synthase gene |
| Answer» C. ACC deaminase | |
| 18. |
What are the various disadvantages of cross protection? |
| A. | Possibility of mutations in inducing mild virus strain |
| B. | Possibility of synergism between inducing virus and other unrelated virus |
| C. | Possibility of unnecessary spread of mild virus |
| D. | All of the above |
| Answer» E. | |
| 19. |
Which of the following gene detoxifies herbicide phosphinothricin? |
| A. | Nitrilase |
| B. | Glutathione S-transferase (GST) |
| C. | Phosphinothricin acetyl transferase |
| D. | All of these |
| Answer» D. All of these | |
| 20. |
ACC demainase gene is responsible for |
| A. | synthesis for ethylene |
| B. | degradation of ACC an immediate precursor to ethylene |
| C. | both (a) and (b) |
| D. | synthesis of polygalactouranase |
| Answer» C. both (a) and (b) | |
| 21. |
Plants derived sexually from the same plant are____ while those derived from somatic tissue from the same plant are____. |
| A. | identical, different |
| B. | different, also different |
| C. | different, identical |
| D. | plants cannot be derived from somatic tissue |
| Answer» D. plants cannot be derived from somatic tissue | |
| 22. |
Random amplified polymorphic DNA (RAPD) is a method that |
| A. | stimulate production of sense RNA to compensate |
| B. | activate the expression of all genes in a biochemical pathway |
| C. | eliminate the expression of all genes in a biochemical pathway |
| D. | reveals intra-specific variation and diversity between species |
| Answer» E. | |
| 23. |
Nitrilase is encoded by |
| A. | gene bxn in Klebsiella pneumoniae |
| B. | bar gene in Streptomyces spp |
| C. | both (a) and (b) |
| D. | none of these |
| Answer» B. bar gene in Streptomyces spp | |
| 24. |
The tumor phenotype, which can be maintained indefinitely in tissue culture, results from the expression of genes on the |
| A. | T-DNA |
| B. | c-DNA |
| C. | r-DNA |
| D. | m-RNA |
| Answer» B. c-DNA | |
| 25. |
The shortest of mitotic phases is the |
| A. | telophase |
| B. | interphase |
| C. | metaphase |
| D. | anaphase |
| Answer» E. | |
| 26. |
Which of the following gene is transferred to plants that detoxify the herbicide atrazine? |
| A. | Nitrilase |
| B. | Glutathione S-transferase (GST) |
| C. | Phosphinothrium acetyl transferase |
| D. | All of these |
| Answer» C. Phosphinothrium acetyl transferase | |
| 27. |
The encapsulation of somatic embryos can be carried out by |
| A. | automatic encapsulation process |
| B. | gel complexation |
| C. | both (a) and (b) |
| D. | coating proteins |
| Answer» D. coating proteins | |
| 28. |
Reducing the expression of pectin methylesterase (PME) has resulted in |
| A. | plants with resistance to bacteria and fungi |
| B. | tomato juice that has higher viscosity |
| C. | tomato fruit that last longer before they become rotten |
| D. | tomato fruit that will not ripen |
| Answer» C. tomato fruit that last longer before they become rotten | |
| 29. |
Human serum albumin has been synthesized by |
| A. | transgenic potato and tobacco plants |
| B. | transgenic maize plants |
| C. | transgenic wheat plants |
| D. | any of the above |
| Answer» B. transgenic maize plants | |
| 30. |
During meiosis crossing over takes place at |
| A. | zygotene stage |
| B. | diplotene stage |
| C. | diakinesis stage |
| D. | pachytene stage |
| Answer» E. | |
| 31. |
When an organism has a life cycle with alternation of generations, the haploid generation is the |
| A. | zygote |
| B. | gametophyte |
| C. | gamete |
| D. | sporophyte |
| Answer» C. gamete | |
| 32. |
A naturally occurring variant, possessing characteristics of interest, is identified. This plant is selectively bred. This is an example of |
| A. | traditional plant breeding |
| B. | transgenic technology |
| C. | mutant selection |
| D. | none of these |
| Answer» B. transgenic technology | |
| 33. |
Expression of antisense RNA in transgenic plants is a general method used to |
| A. | activate the expression of all genes in a biochemical pathway |
| B. | eliminate the expression of all genes in a biochemical pathway |
| C. | block the expression of virus coat protein genes |
| D. | reduce or eliminate the expression of individual genes |
| Answer» E. | |
| 34. |
The delayed ripening tomato was created by a biotechnologist who __________ a gene. |
| A. | altered |
| B. | silenced |
| C. | replaced |
| D. | relocated |
| Answer» C. replaced | |
| 35. |
When F1 plants are crossed with homozygous recessive plants, it is known as |
| A. | test cross |
| B. | recessive cross |
| C. | criss-cross |
| D. | para-cross |
| Answer» B. recessive cross | |
| 36. |
When do kinetochore microtubules pull chromosomes towards opposite spindle poles? |
| A. | In anaphase A |
| B. | In anaphase B |
| C. | In metaphase |
| D. | In telophase |
| Answer» B. In anaphase B | |
| 37. |
Resistance to glyphosphate in transgenic petunia plants has been developed by the transfer of |
| A. | gene for EPSPS(5-enol-pyruvyl shikimat 3 phosphate synthase) |
| B. | gene for ALS (acetolactate synthase) |
| C. | gene for GS (glutamine synthase) |
| D. | any of the above |
| Answer» B. gene for ALS (acetolactate synthase) | |
| 38. |
Which of the following genes can be used for making resistances against viral infection? |
| A. | Genes for capsid protein |
| B. | Gene for nucleocapsid protein |
| C. | Satellite RNA |
| D. | All of these |
| Answer» E. | |
| 39. |
Tomatoes exhibiting delayed ripening express antisense RNA against |
| A. | glycerol 1 phosphate acyl transferase |
| B. | polygalactouranase |
| C. | ACC deaminase |
| D. | sucrose phosphate synthase gene |
| Answer» D. sucrose phosphate synthase gene | |
| 40. |
Which of the following is not true about synthetic seeds? |
| A. | Can be stored for a year without the loss of valuables |
| B. | Easy to handle |
| C. | Can be directly sown in the soil like natural seeds |
| D. | Need hardening in the green house |
| Answer» E. | |
| 41. |
What is the approximate size (in kb) of the melon mitochondrial genome? |
| A. | 600 kilobase |
| B. | 1200 kilobase |
| C. | 2400 kilobase |
| D. | 3000 kilobase |
| Answer» D. 3000 kilobase | |
| 42. |
Which of the following transfers a set of genes encoded in a region called T-DNA of the Ti-plasmid into cells at wound positions? |
| A. | A. tumefaciens |
| B. | L. peruvianum |
| C. | L. birsutum |
| D. | A. sterilis |
| Answer» B. L. peruvianum | |
| 43. |
Which of the following gene detoxify herbicide bronoxynil? |
| A. | Nitrilase |
| B. | Glutathione S-transferase (GST) |
| C. | Phosphinothricin acetyl transferase |
| D. | All of these |
| Answer» B. Glutathione S-transferase (GST) | |
| 44. |
Starch content of potatoes can be increased by using a bacterial gene, known as |
| A. | sucrose phosphate synthase gene |
| B. | ADP glucose pyrophosphorylase gene |
| C. | polygalactouranase gene |
| D. | none of the above |
| Answer» C. polygalactouranase gene | |
| 45. |
The first transgenic plants expressing engineered foreign genes were tobacco plants produced by the use of |
| A. | Agrobacterium tumefaciens |
| B. | Bacillus thuringiensis |
| C. | Arabidopsis thaliana |
| D. | Streptomyces hygroscopicus |
| Answer» B. Bacillus thuringiensis | |
| 46. |
Which of the following technique can be used to develop nematode resistant plants? |
| A. | Embryo-rescue |
| B. | Mutation |
| C. | Non-recombinant DNA |
| D. | All of these |
| Answer» E. | |
| 47. |
For increased level of mannitol in transgenic tobacco plants which of the following gene is transferred? |
| A. | Mannitol dehydrogenase |
| B. | Mannitol synthease |
| C. | Both (a) and (b) |
| D. | Mannitol pyrophosphorylase |
| Answer» B. Mannitol synthease | |
| 48. |
When the transposon jumps into an otherwise functional gene, it has the effect of |
| A. | blocking correct transcription |
| B. | initiating correct transcription |
| C. | propagating correct transcription |
| D. | initiating some genetic codes |
| Answer» B. initiating correct transcription | |
| 49. |
Direct DNA uptake by protoplasts can be stimulated by |
| A. | polyethylene glycol (PEG) |
| B. | decanal |
| C. | luciferin |
| D. | all of these |
| Answer» B. decanal | |
| 50. |
Which of the following is not true about the helper plasmids? |
| A. | These can replicate in Agrobacterium |
| B. | These help in the mediating conjugation of intermediate vectors |
| C. | These can't replicate in Agrobacterium |
| D. | All of the above |
| Answer» B. These help in the mediating conjugation of intermediate vectors | |