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				This section includes 87 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.
| 1. | If complex III were incorporated into an artificial lipid vesicle in order to demonstrate it's proton translocating ability in isolation, which of the following would be an appropriate electron donor? | 
| A. | Cytochrome c red | 
| B. | Cytochrome c peroxidase | 
| C. | Ubiquinol (UQH2) | 
| D. | Cytochrome c oxidase | 
| Answer» D. Cytochrome c oxidase | |
| 2. | Which of the following experimental results does not support Mitchell's chemiosmosis theory? | 
| A. | Electron transport in isolated mitochondria was shown to result in acidification of the medium | 
| B. | Addition of dinitrophenol to isolated mitochondria was shown to inhibit electron transport, but had no effect on | 
| C. | synthesis | 
| D. | Addition of dinitrophenol to isolated mitochondria during electron transport was shown to inhibit acidification of the medium | 
| E. | An artificial proton gradient across the inner mitochondrial membrane drives ATP synthesis in the absence of electron transport | 
| Answer» C. synthesis | |
| 3. | "The universe tends towards maximum disorder" is stated by | 
| A. | first law of thermodynamics | 
| B. | second law of thermodynamics | 
| C. | third law of thermodynamics | 
| D. | none of the above | 
| Answer» C. third law of thermodynamics | |
| 4. | If the ester bonds of a triacylglycerol molecule's are broken then the kind of molecule, which would be soluble in water, is | 
| A. | fatty acid | 
| B. | steroid | 
| C. | glycogen | 
| D. | glycerol | 
| Answer» E. | |
| 5. | Agrobacterium based gene transfer is efficient | 
| A. | only with dicots | 
| B. | only with monocots | 
| C. | with both monocots and dicots | 
| D. | with majority monocots and few dicots | 
| Answer» B. only with monocots | |
| 6. | Which method of plant propagation involves the use of girdling? | 
| A. | Grafting | 
| B. | Cuttings | 
| C. | Layering | 
| D. | Micropropagation | 
| Answer» D. Micropropagation | |
| 7. | Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation? | 
| A. | Multiplication of sexually derived sterile hybrids | 
| B. | Less multiplication of disease free plants | 
| C. | Storage and transportation of propogates | 
| D. | Both (b) and (c) | 
| Answer» D. Both (b) and (c) | |
| 8. | Which of the following is not true about nurse or conditioned medium? | 
| A. | It is liquid removed from the suspension of fast growing cells | 
| B. | It contains uncharacterized growth factor released by growing cells | 
| C. | It is used in the culture of regenerating protoplast | 
| D. | it is removed aseptically from the culture and is autoclaved before use | 
| Answer» E. | |
| 9. | When plated only in nutrient medium, how much time is required for the protoplast to synthesize new cell wall? | 
| A. | 2-5 days | 
| B. | 5-10 days | 
| C. | 10-15 days | 
| D. | 15-17 days | 
| Answer» C. 10-15 days | |
| 10. | The ability of the component cells of callus to form a whole plant is known as | 
| A. | redifferentiation | 
| B. | dedifferentiation | 
| C. | either (a) or (b) | 
| D. | none of these | 
| Answer» B. dedifferentiation | |
| 11. | Which of the following is not a cytokinin? | 
| A. | 2,4-dichlorophenoxyacetic acid | 
| B. | 6 benzylaminopurine | 
| C. | Zeatin | 
| D. | Kinetin | 
| Answer» B. 6 benzylaminopurine | |
| 12. | Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media | 
| A. | utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise | 
| B. | utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise | 
| C. | utilization of both ammonium and nitrate result in rise in pH | 
| D. | utilization of both ammonium and nitrate result in drop in pH | 
| Answer» B. utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise | |
| 13. | Which breeding method uses a chemical to strip the cell wall of plant cells of two sexually incompatible species? | 
| A. | Mass selection | 
| B. | Protoplast fusion | 
| C. | Transformation | 
| D. | Transpiration | 
| Answer» C. Transformation | |
| 14. | In a callus culture | 
| A. | increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation | 
| B. | increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation | 
| C. | auxins and cytokinins are not required | 
| D. | only auxin is required for root and shoot formation | 
| Answer» B. increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation | |
| 15. | When guard cells swell | 
| A. | stomata close | 
| B. | stomata open | 
| C. | photosynthesis stops | 
| D. | evaporation stops | 
| Answer» C. photosynthesis stops | |
| 16. | The evaporation of water from leaves is called | 
| A. | transpiration | 
| B. | cohesion | 
| C. | tension | 
| D. | pressure flow | 
| Answer» B. cohesion | |
| 17. | Which of the following statements about the outer mitochondrial membrane is not true? | 
| A. | The outer membrane is impermeable to hydrogen ions | 
| B. | The outer membrane is highly permeable to substances of small molecular weight | 
| C. | The outer membrane is about 50% lipid and 50% protein by weight | 
| D. | The outer membrane contains porins | 
| Answer» B. The outer membrane is highly permeable to substances of small molecular weight | |
| 18. | The cell membrane is made up of | 
| A. | lipoproteins | 
| B. | cellulose | 
| C. | phospholipids | 
| D. | phosphoproteins | 
| Answer» B. cellulose | |
| 19. | Cellular totipotency is the property of | 
| A. | plants | 
| B. | animals | 
| C. | bacteria | 
| D. | all of these | 
| Answer» B. animals | |
| 20. | Which is/are the naturally occurring plant auxins? | 
| A. | Indole acetic acid (IAA) | 
| B. | Naphthalenacetic acid (NAA) | 
| C. | 2,4-dichlorophenoxyacetic acid | 
| D. | All of the above | 
| Answer» B. Naphthalenacetic acid (NAA) | |
| 21. | Which of the following growth regulator promote cell division? | 
| A. | Auxins | 
| B. | Cytokinins | 
| C. | Gibberellins | 
| D. | Brassinosteroids | 
| Answer» C. Gibberellins | |
| 22. | If complex III were incorporated into an artificial lipid vesicle in order to demonstrate it's proton translocating ability in isolation, which of the following would be an appropriate electron donor? | 
| A. | Cytochrome c red | 
| B. | Cytochrome c peroxidase | 
| C. | Ubiquinol (UQH2) | 
| D. | Cytochrome c oxidase | 
| Answer» D. Cytochrome c oxidase | |
| 23. | A(n) __________ is an excised piece of leaf or stem tissue used in micropropagation. | 
| A. | microshoot | 
| B. | medium | 
| C. | explant | 
| D. | scion | 
| Answer» D. scion | |
| 24. | What is/are the benefit(s) of micropropagation or clonal propagation? | 
| A. | Rapid multiplication of superior clones | 
| B. | Multiplication of disease free plants | 
| C. | Multiplication of sexually derived sterile hybrids | 
| D. | All of the above | 
| Answer» E. | |
| 25. | If the ester bonds of a triacylglycerol molecule's are broken then the kind of molecule, which would be soluble in water, is | 
| A. | fatty acid | 
| B. | steroid | 
| C. | glycogen | 
| D. | glycerol | 
| Answer» E. | |
| 26. | Cellular organelles are embedded in the | 
| A. | endoplasmic reticulum | 
| B. | cristae | 
| C. | cytosol | 
| D. | microtubules | 
| Answer» D. microtubules | |
| 27. | The cellular organelle associated with photosynthesis is the | 
| A. | golgi complex | 
| B. | mitochondrion | 
| C. | lysosome | 
| D. | chloroplast | 
| Answer» E. | |
| 28. | A cell without cell wall is called | 
| A. | apoplast | 
| B. | protoplast | 
| C. | symplast | 
| D. | none of these | 
| Answer» C. symplast | |
| 29. | Which cell type is likely to have the most mitochondria? | 
| A. | Fast-twitch muscle | 
| B. | Slow-twitch muscle | 
| C. | Liver cells | 
| D. | Adipose cells | 
| Answer» C. Liver cells | |
| 30. | Tonoplast is a membrane around | 
| A. | cytoplasm | 
| B. | vacuole | 
| C. | nucleus | 
| D. | mitochondria | 
| Answer» C. nucleus | |
| 31. | Which part of a plant contains the male sex cell? | 
| A. | Ova | 
| B. | Style | 
| C. | Stamen | 
| D. | Pollen | 
| Answer» E. | |
| 32. | Chemical energy is supplied to eukaryotic cells by | 
| A. | chloroplasts and golgi complexes | 
| B. | mitochondria and ribosomes | 
| C. | mitochondria only | 
| D. | mitochondria and chloroplasts | 
| Answer» E. | |
| 33. | The movement of most minerals, including potassium into root cells is by | 
| A. | osmosis | 
| B. | diffusion | 
| C. | bulk flow | 
| D. | active transport | 
| Answer» E. | |
| 34. | What are the macronutrients used in plant cell culture medium? | 
| A. | N, P, K, S, Na | 
| B. | N, P, K, Ca, Cl | 
| C. | N, P, K, S, Ca | 
| D. | N, P, Ca, Na, Cl | 
| Answer» D. N, P, Ca, Na, Cl | |
| 35. | Which of the following growth regulator is added for short initiation during plant regeneration from callus? | 
| A. | Auxins | 
| B. | Cytokinins | 
| C. | Gibberellins | 
| D. | Brassinosteroids | 
| Answer» C. Gibberellins | |
| 36. | The cohesion-tension theory of water movement helps to explain how water moves from soil into root? | 
| A. | Through the phloem | 
| B. | From leaf to root | 
| C. | Through the xylem | 
| D. | Into the guard cells | 
| Answer» D. Into the guard cells | |
| 37. | Which of the following growth regulator cause plant cells to grow? | 
| A. | Auxins | 
| B. | Cytokinins | 
| C. | Gibberellins | 
| D. | Brassinosteroids | 
| Answer» B. Cytokinins | |
| 38. | Which of the following observations is not used as evidence to support the concept of endosymbiotic origin of chloroplasts and mitochondria? | 
| A. | Both have their own DNA | 
| B. | Both have their own ribosomes | 
| C. | Both strongly resemble prokaryotes, especially in size | 
| D. | Both can live and function independently of the cell | 
| Answer» E. | |
| 39. | Mitochondria can be expected most numerous in | 
| A. | green cells | 
| B. | red blood cells | 
| C. | muscle cells | 
| D. | none of these | 
| Answer» D. none of these | |
| 40. | The element neon (Ne) has eight electrons in its outermost electron shell. How many covalent bonds will Ne readily form? | 
| A. | None | 
| B. | One | 
| C. | Two | 
| D. | Four | 
| Answer» B. One | |
| 41. | The cellular organelle associated with secretion is the | 
| A. | golgi complex | 
| B. | mitochondrion | 
| C. | ribosome | 
| D. | lysosome | 
| Answer» B. mitochondrion | |
| 42. | A widely accepted explanation for transport of sugar in phloem is called | 
| A. | root pressure theory | 
| B. | transpiration | 
| C. | pressure flow hypothesis | 
| D. | cytoplasmic streaming | 
| Answer» D. cytoplasmic streaming | |
| 43. | The fluid matrix of a cell is called the | 
| A. | nucleus | 
| B. | cytosol | 
| C. | cytoskeleton | 
| D. | endoplasmic reticulum | 
| Answer» C. cytoskeleton | |
| 44. | Specialized type of peroxisome and the site of glyoxylate pathway in certain plants is known as | 
| A. | gly-peroxisome | 
| B. | lysosome | 
| C. | glyoxysome | 
| D. | none of these | 
| Answer» D. none of these | |
| 45. | Which is the most common carbon source used in the plant cell culture media? | 
| A. | Sucrose | 
| B. | Glucose | 
| C. | Fructose | 
| D. | Maltose | 
| Answer» B. Glucose | |
| 46. | Any system that is not at equilibrium | 
| A. | is thermodynamically unstable, although it may be kinetically stable | 
| B. | is kinetically unstable, although it may be thermodynamically stable | 
| C. | is rushing toward equilibrium at a very rapid rate | 
| D. | requires an enzyme to reach equilibrium | 
| Answer» B. is kinetically unstable, although it may be thermodynamically stable | |
| 47. | Neutralized activated charcoal is occasionally added to young regenerating cultures to | 
| A. | remove toxic phenolics produced by the stressed plant cell | 
| B. | help to remove plants growth regulators introduced at an earlier stage | 
| C. | both (a) and (b) | 
| D. | maintain the pH of the medium | 
| Answer» D. maintain the pH of the medium | |
| 48. | Which molecule serves as a carrier of fatty acids in mitochondria? | 
| A. | Acetyl-CoA | 
| B. | Carnitine | 
| C. | Dolichol | 
| D. | Malonyl-CoA | 
| Answer» C. Dolichol | |
| 49. | Phloem is composed of | 
| A. | vessel elements and companion cells | 
| B. | tracheids and vessel elements | 
| C. | sieve-tube elements and companion cells | 
| D. | tracheids and sieve-tube elements | 
| Answer» D. tracheids and sieve-tube elements | |
| 50. | Water moves into roots of plants by | 
| A. | osmosis | 
| B. | active transport | 
| C. | diffusion | 
| D. | pressure flow mechanism | 
| Answer» B. active transport | |