MCQOPTIONS
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MCQOPTIONS
This section includes 11 Mcqs, each offering curated multiple-choice questions to sharpen your Bioinformatics knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which of the following is untrue regarding SAGE? |
| A. | It stands for Serial analysis of gene expression |
| B. | It is another high throughput, sequence-based approach for global gene expression profile analysis |
| C. | It stands for Squared analysis of gene expression |
| D. | Unlike EST sampling, SAGE is more quantitative in determining mRNA expression in a cell |
| Answer» D. Unlike EST sampling, SAGE is more quantitative in determining mRNA expression in a cell | |
| 2. |
Which of the following is untrue regarding TIGR Gene Indices? |
| A. | It is an EST database that the similar type of clustering method from UniGene |
| B. | It is an EST database that uses a different clustering method from UniGene |
| C. | It compiles data from dbEST, GenBank mRNA and genomic DNA data, and TIGR’s own sequence databased Sequences are only clustered if they are more than 95% identical for over a fortynucleotide region in pairwise comparisons |
| D. | None of the mentioned |
| Answer» B. It is an EST database that uses a different clustering method from UniGene | |
| 3. |
WHICH_OF_THE_FOLLOWING_IS_UNTRUE_REGARDING_TIGR_GENE_INDICES??$ |
| A. | It is an EST database that the similar type of clustering method from UniGene |
| B. | It is an EST database that uses a different clustering method from UniGene |
| C. | It compiles data from dbEST, GenBank mRNA and genomic DNA data, and TIGR’s own sequence databased) Sequences are only clustered if they are more than 95% identical for over a fortynucleotide region in pairwise comparisons |
| Answer» B. It is an EST database that uses a different clustering method from UniGene | |
| 4. |
Which_of_the_following_is_untrue_regarding_SAGE?$ |
| A. | It stands for Serial analysis of gene expression |
| B. | It is another high throughput, sequence-based approach for global gene expression profile analysis |
| C. | It stands for Squared analysis of gene expression |
| D. | Unlike EST sampling, SAGE is more quantitative in determining mRNA expression in a cell |
| Answer» D. Unlike EST sampling, SAGE is more quantitative in determining mRNA expression in a cell | |
| 5. |
Which of the following is untrue regarding UniGene? |
| A. | It is an NCBI EST cluster database. |
| B. | Overlapping EST sequences are computationally processed to represent a single expressed gene. |
| C. | Each cluster is a set of overlapping EST sequences |
| D. | The overlapping EST sequences are computationally processed to represent a set of expressed genes |
| Answer» E. | |
| 6. |
Which of the following is untrue regarding EST Index Construction? |
| A. | The goal of the EST databases is to organize and consolidate the largely redundant EST data |
| B. | The process includes a preprocessing step that removes masks repeats |
| C. | There is no screening of vector contaminants |
| D. | The goal of the EST databases is to improve the quality of the sequence information so the data can be used to extract full-length cDNAs |
| Answer» D. The goal of the EST databases is to improve the quality of the sequence information so the data can be used to extract full-length cDNAs | |
| 7. |
GenBank has a special EST database, dbEST that contains EST collections for a large number of organisms. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 8. |
It has been estimated that up to 11% of cDNA clones may be chimeric. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 9. |
Which of the following is untrue regarding the drawbacks of expressed sequence tags (ESTs)? |
| A. | They are often of lowquality because they are automatically generated without verification |
| B. | Many bases are ambiguously determined, represented by N’s |
| C. | Frame shift errors and artifactual stop codons are some common errors |
| D. | Despite of all the failures, the translation the sequences is smooth |
| Answer» E. | |
| 10. |
To generate EST data, clones in the cDNA library are randomly selected for sequencing from either end of the inserts. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 11. |
Which of the following is untrue regarding expressed sequence tags (ESTs)? |
| A. | One of the high throughput approaches to genome-wide profiling of gene expression is sequencing ESTs |
| B. | They are short sequences obtained from cDNA clones |
| C. | They serve as short identifiers of full-length genes |
| D. | They are typically in the range of 800 to 900 nucleotides in length |
| Answer» E. | |