MCQOPTIONS
Saved Bookmarks
MCQOPTIONS
This section includes 18 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
DpnI cuts ________ strands. |
| A. | methylated |
| B. | non-methylated |
| C. | phosphorylated |
| D. | non-phosphorylated |
| Answer» B. non-methylated | |
| 2. |
What is the function of ung gene? |
| A. | It is responsible for deamination of cytosine |
| B. | It is responsible for deamination of uracil |
| C. | It is responsible for removal of uracil |
| D. | It is responsible for removal of cytosine |
| Answer» D. It is responsible for removal of cytosine | |
| 3. |
In a phosphothiorate nucleotide, oxygen atom is replaced by with atom? |
| A. | Magnesium |
| B. | Calcium |
| C. | Sodium |
| D. | Sulphur |
| Answer» E. | |
| 4. |
Replication by first strand leads to the formation of mutated molecules and functional antibiotic resistant gene. |
| A. | True |
| B. | False |
| Answer» C. | |
| 5. |
Once second strand synthesis is carried out, it is introduced into the host. Host is having which mutation? |
| A. | mutS mutation |
| B. | mutD mutation |
| C. | mutE mutation |
| D. | mutG mutation |
| Answer» B. mutD mutation | |
| 6. |
If PCR is used to introduce random mutations rather than specific mutations, it is called as ___________ |
| A. | mutagenic PCR |
| B. | error-prone PCR |
| C. | random PCR |
| D. | general PCR |
| Answer» C. random PCR | |
| 7. |
PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a product corresponding to the part of the gene. It is called as megaprimer. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 8. |
REPLICATION_BY_FIRST_STRAND_LEADS_TO_THE_FORMATION_OF_MUTATED_MOLECULES_AND_FUNCTIONAL_ANTIBIOTIC_RESISTANT_GENE._IS_THE_GIVEN_STATEMENT_TRUE_OR_FALSE??$ |
| A. | True |
| B. | False |
| Answer» C. | |
| 9. |
What is the function of ung gene?$ |
| A. | It is responsible for deamination of cytosine |
| B. | It is responsible for deamination of uracil |
| C. | It is responsible for removal of uracil |
| D. | It is responsible for removal of cytosine |
| Answer» D. It is responsible for removal of cytosine | |
| 10. |
In_a_phosphothiorate_nucleotide,_oxygen_atom_is_replaced_by_with_atom?$ |
| A. | Magnesium |
| B. | Calcium |
| C. | Sodium |
| D. | Sulphur |
| Answer» E. | |
| 11. |
DpnI_cuts__________strands. |
| A. | methylated |
| B. | non-methylated |
| C. | phosphorylated |
| D. | non-phosphorylated |
| Answer» B. non-methylated | |
| 12. |
Once second strand synthesis is carried out, it is introduced into host. Host is having which mutation? |
| A. | mutS mutation |
| B. | mutD mutation |
| C. | mutE mutation |
| D. | mutG mutation |
| Answer» B. mutD mutation | |
| 13. |
Choose the incorrect statement for the methodology of selection of molecules with mutant sequences. |
| A. | A vector is used which is having antibiotic resistance gene |
| B. | Apart from antibiotic resistance gene, a second antibiotic resistance gene is also present |
| C. | There are two mutagenic primers which are used |
| D. | The second strand synthesis is carried out by only one primer |
| Answer» E. | |
| 14. |
For the selection of the molecules having mutated sequence, which of the statement is true? |
| A. | It is suitable for methods which are PCR based |
| B. | It is suitable for methods which are not PCR based |
| C. | It is suitable for both PCR and not PCR based |
| D. | Selection of molecules with mutant sequence is not possible |
| Answer» C. It is suitable for both PCR and not PCR based | |
| 15. |
If PCR is used to introduce random mutations rather than specific mutations, it is called as: |
| A. | mutagenic PCR |
| B. | error-prone PCR |
| C. | random PCR |
| D. | general PCR |
| Answer» C. random PCR | |
| 16. |
Sometimes mutagenesis is carried out with the help of primers. Choose the correct statement with respect to it. |
| A. | Double stranded circular molecule is used as a template |
| B. | Mutation is introduced into one of flanking primers |
| C. | Single stranded circular molecule is used as a template |
| D. | After amplification, mutation is introduced into one strand |
| Answer» B. Mutation is introduced into one of flanking primers | |
| 17. |
PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a product corresponding to the part of the gene. It is called as megaprimer. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 18. |
A megaprimer method is a ____ stage approach and uses _____ oligonucleotide primers. |
| A. | two, two |
| B. | two, three |
| C. | one, two |
| D. | one, three |
| Answer» C. one, two | |