Choose the incorrect statement for panning.

It is a variant of ligand binding approach

The ligand is immobilized on the solid support and the cells are passed over it

If the ligand-binding domain is exposed on surface of the any recombinant molecules then they will bind to ligand

They are not useful for scanning libraries in cultured mammalian cells

In the case of ligand binding by the expressed protein, the library can be screened by ______________

immunochemically, by the use of antibodies

ligands if the sequence we are looking for encodes a protein specific to a ligand

using a specific DNA sequence which can bind to the protein encoded by the sequence of interest

Xenopus oocyte cells are also used at the time to carry out screening. Choose the incorrect statement for this procedure.

The screening is helpful for the proteins whose function can be easily screened such as transport of ions

DNA from collections is transcribed within in vitro and the transcription products are microinjected into oocytes

The RNA is translated within oocytes

Screening is carried out on the basis of DNA

The screening is helpful for the proteins whose function can be easily screened such as transport of ions

Choose the incorrect statement with respect to host used.

The host should be deficient in recombination

The host must carry mutation for the gene of interest

The host should not contain restriction enzymes

The host if having restriction enzymes, then the incoming DNA should not be methylated

The host should be deficient in recombination

Explore topic-wise MCQs in Genetic Engineering.

This section includes 9 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.	If screening is carried out by using a combination of nascent peptide and mRNA, then it is called as ________
A.	nascent peptide display
B.	mRNA display
C.	ribosome display
D.	ribozyme display
Answer» D. ribozyme display

Discussion

2.	Choose the incorrect statement for panning.
A.	It is a variant of ligand binding approach
B.	The ligand is immobilized on the solid support and the cells are passed over it
C.	If the ligand-binding domain is exposed on surface of the any recombinant molecules then they will bind to ligand
D.	They are not useful for scanning libraries in cultured mammalian cells
Answer» E.

Discussion

3.	The ligand should bind to a single protein only for the screening process.
A.	True
B.	False
Answer» B. False

Discussion

4.	In the case of immunochemical screening, the position of ________ antibody is detected by ________ antibody.
A.	secondary, primary
B.	primary, secondary
C.	primary, tertiary
D.	secondary, tertiary
Answer» C. primary, tertiary

Discussion

5.	In the case of ligand binding by the expressed protein, the library can be screened by ______________
A.	immunochemically, by the use of antibodies
B.	ligands if the sequence we are looking for encodes a protein specific to a ligand
C.	using a specific DNA sequence which can bind to the protein encoded by the sequence of interest
D.	all of the mentioned
Answer» E.

Discussion

6.	Xenopus oocyte cells are also used at the time to carry out screening. Choose the incorrect statement for this procedure.
A.	DNA from collections is transcribed within in vitro and the transcription products are microinjected into oocytes
B.	The RNA is translated within oocytes
C.	Screening is carried out on the basis of DNA
D.	The screening is helpful for the proteins whose function can be easily screened such as transport of ions
Answer» D. The screening is helpful for the proteins whose function can be easily screened such as transport of ions

Discussion

7.	If the library to be screened and the host belongs to different species, then it is called as ___________
A.	homologous selection
B.	heterologous selection
C.	intraspecies selection
D.	mixed selection
Answer» C. intraspecies selection

Discussion

8.	If the gene to be screened belongs to prokaryotic species then there are chances that it is not expressed.
A.	True
B.	False
Answer» B. False

Discussion

9.	Choose the incorrect statement with respect to host used.
A.	The host must carry mutation for the gene of interest
B.	The host should not contain restriction enzymes
C.	The host if having restriction enzymes, then the incoming DNA should not be methylated
D.	The host should be deficient in recombination
Answer» D. The host should be deficient in recombination

Discussion

Explore topic-wise MCQs in Genetic Engineering.

If screening is carried out by using a combination of nascent peptide and mRNA, then it is called as ________

Choose the incorrect statement for panning.

The ligand should bind to a single protein only for the screening process.

In the case of immunochemical screening, the position of ________ antibody is detected by ________ antibody.

In the case of ligand binding by the expressed protein, the library can be screened by ______________

Xenopus oocyte cells are also used at the time to carry out screening. Choose the incorrect statement for this procedure.

If the library to be screened and the host belongs to different species, then it is called as ___________

If the gene to be screened belongs to prokaryotic species then there are chances that it is not expressed.

Choose the incorrect statement with respect to host used.

In the case of immunochemical screening, the position of antibody is detected by antibody.