MCQOPTIONS
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MCQOPTIONS
This section includes 12 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
How can phosphatase activity terminated prior to a ligation reaction? |
| A. | By heating |
| B. | By cooling |
| C. | By creating vibrations |
| D. | By alternatively cooling and heating |
| Answer» B. By cooling | |
| 2. |
How is phosphatase related to the ligation reactions? |
| A. | Phosphate group is not required for the ligation reaction to take place, thus phosphatase is helpful |
| B. | It is helpful in ceasing the unwanted ligation |
| C. | Phosphatases are not at all related to ligation reactions |
| D. | They act as a catalyst in case of the ligation reaction |
| Answer» C. Phosphatases are not at all related to ligation reactions | |
| 3. |
Phosphatases refer to __________ |
| A. | the enzymes which add phosphate group at the end of the DNA molecule in the place of hydroxyl group |
| B. | the enzymes which hydrolytically remove phosphate group from the DNA molecules and replace them with hydroxyl group |
| C. | the enzymes responsible for removal of phosphate group from the DNA molecules and replace them with hydrogen |
| D. | the enzymes responsible for replacing hydrogen in the DNA molecules with the phosphate group |
| Answer» C. the enzymes responsible for removal of phosphate group from the DNA molecules and replace them with hydrogen | |
| 4. |
Besides enzymatic means, physical stress can also be used for cleaving the DNA. Which of the following statements is true? |
| A. | Sonication, needles, syringes, etc all come under the category of physical stress |
| B. | In physical stress, there are no chances of contamination |
| C. | The ends obtained have unique sequences |
| D. | They are less effective than DNase treatment |
| Answer» B. In physical stress, there are no chances of contamination | |
| 5. |
The ends created by use of DNase have unique single stranded sequences. |
| A. | True |
| B. | False |
| Answer» C. | |
| 6. |
Why is DNase preferred over restriction endonuclease in some cases? |
| A. | DNase is preferred over restriction endonuclease in some cases because the latter are not able to recognize some of the restriction sites |
| B. | DNase is more specific as restriction endonuclease, so the required fragment is obtained |
| C. | DNase is less specific as compared to restriction endonuclease, hence there are more chances of representation of all the possible fragments |
| D. | DNase is abundant in comparison the restriction endonuclease |
| Answer» D. DNase is abundant in comparison the restriction endonuclease | |
| 7. |
Which of the following is the correct nomenclature of a restriction enzyme obtained from the first activity of strain R of Escherichia coli? |
| A. | EcoR1 |
| B. | EscRI |
| C. | EcorI |
| D. | EcoRI |
| Answer» E. | |
| 8. |
Which of the following statement is correct regarding partial digestion? |
| A. | It is defined as the conditions where all the sites in the DNA sequence are not recognized |
| B. | The number of fragments created by partial digestion are same as that of complete digestion |
| C. | It is not useful in representation of genomic library |
| D. | Exactly half of the sites in the DNA are recognized |
| Answer» B. The number of fragments created by partial digestion are same as that of complete digestion | |
| 9. |
Which of the following statements is correct with respect to a unit of an enzyme? |
| A. | One unit of enzyme is defined as the amount of enzyme required to digest 1miligram of standard DNA in a specific time of 1hr and under given temperature conditions |
| B. | The amount of enzyme required doesn t vary with the number of sites present in the DNA |
| C. | If more number of sites is there in the DNA more units of enzyme are required in comparison to same amount of DNA with fewer sites |
| D. | The amount of enzyme required for digestion of DNA with less number of sites is more than that of more number of sites in the same amount of DNA |
| Answer» D. The amount of enzyme required for digestion of DNA with less number of sites is more than that of more number of sites in the same amount of DNA | |
| 10. |
The specificity of an enzyme is affected by the concentration of buffer used. This phenomenon is termed as: |
| A. | star activity |
| B. | specificity elevation |
| C. | concentration gradient effects |
| D. | diamond activity |
| Answer» B. specificity elevation | |
| 11. |
Isocaudomers are defined as the enzymes which recognize different sequence but generate same ends. Which of the following pairs of enzymes can be termed as isocuadomers? |
| A. | DpnI (GA|TC) and Sau3A (|GATC) |
| B. | BamHI (G|GATCC) and Sau3A (|GATC) |
| C. | DpnI (GA|TC) and BglII (A|GATCT) |
| D. | XbaI (T|CTAGA) and BamHI (G|GATCC) |
| Answer» C. DpnI (GA|TC) and BglII (A|GATCT) | |
| 12. |
Isoschizomers are defined as ___________ |
| A. | enzymes having same recognition sequence and always cutting at the same site |
| B. | enzymes having same recognition sequence and always cutting at different site |
| C. | enzymes having different recognition site and cutting at the same site |
| D. | enzymes having same recognition site and they may or may not cut at the same site |
| Answer» E. | |