Explore topic-wise MCQs in Bioinformatics.

This section includes 14 Mcqs, each offering curated multiple-choice questions to sharpen your Bioinformatics knowledge and support exam preparation. Choose a topic below to get started.

1.

Which of the following is untrue regarding the STRING?

A. Search Tool for the Retrieval of Interacting Genes/Proteins
B. Functional associations include only the direct protein-protein interactions
C. It is based on combined evidence of gene linkage, gene fusion and phylogenetic profiles
D. It is a web server that predicts gene and protein functional associations
Answer» C. It is based on combined evidence of gene linkage, gene fusion and phylogenetic profiles
Discussion
2.

Which of the following is untrue regarding the predicting Interactions Based on Phylogenetic Information?

A. Proteins do not operate as a complex
B. This method detects the co-presence or co-absence of orthologs across a number of genomes
C. Protein interactions can be predicted using phylogenetic profiles
D. Phylogenetic profile are defined as patterns of gene pairs that are concurrently present or absent across genomes
Answer» B. This method detects the co-presence or co-absence of orthologs across a number of genomes
Discussion
3.

In Predicting Interactions Based on Gene Neighbors if a certain gene linkage is found to be indeed conserved across divergent genomes, it can be used as a strong indicator of formation of an operon that encodes proteins that are functionally and even physically coupled.

A. True
B. False
Answer» B. False
Discussion
4.

Which of the following is untrue regarding the Predicting Interactions Based on Domain Fusion?

A. It is based on gene fusion events
B. Predicting protein–protein interactions is called the “Rosetta stone” method
C. A fused protein often reveals relationships between its domain components
D. A fused protein doesn’t necessarily reveal about the relationships between its domain components
Answer» E.
Discussion
5.

An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column.

A. True
B. False
Answer» B. False
Discussion
6.

If the bait and prey proteins _______ they bring the DNA-binding and trans-activation domains in such close proximity that they reconstitute the function of the transcription activator, turning ____ the expression of a reporter gene as a result. Which of the following is not the correct pair of blanks?

A. physically interact, on
B. do not interact, on
C. do not interact, off
D. stop interacting, off
Answer» C. do not interact, off
Discussion
7.

WHICH_OF_THE_FOLLOWING_IS_UNTRUE_REGARDING_THE_PREDICTING_INTERACTIONS_BASED_ON_PHYLOGENETIC_INFORMATION??$

A. Proteins do not operate as a complex
B. This method detects the co-presence or co-absence of orthologs across a number of genomes
C. Protein interactions can be predicted using phylogenetic profiles
D. Phylogenetic profile are defined as patterns of gene pairs that are concurrently present or absent across genomes
Answer» B. This method detects the co-presence or co-absence of orthologs across a number of genomes
Discussion
8.

Which_of_the_following_is_untrue_regarding_the_STRING?$

A. Search Tool for the Retrieval of Interacting Genes/Proteins
B. Functional associations include only the direct protein-protein interactions
C. It is based on combined evidence of gene linkage, gene fusion and phylogenetic profiles
D. It is a web server that predicts gene and protein functional associations
Answer» C. It is based on combined evidence of gene linkage, gene fusion and phylogenetic profiles
Discussion
9.

In Predicting Interactions Based on Gene Neighbors– if a certain gene linkage is found to be indeed conserved across divergent genomes, it can be used as a strong indicator of formation of an operon that encodes proteins that are functionally and even physically coupled?#

A. True
B. False
Answer» B. False
Discussion
10.

The justification behind Rosetta stone method is that when two domains are fused in a single protein, they have to be in _______ proximity to perform a common function.

A. distant
B. close
C. extremely distant
D. extremely close
Answer» E.
Discussion
11.

When the two domains are located in two different proteins, to preserve the same functionality, their close proximity and interaction have to be preserved as well.

A. True
B. False
Answer» B. False
Discussion
12.

An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column.$

A. True
B. False
Answer» B. False
Discussion
13.

If the bait and prey proteins _______ they bring the DNA-binding and trans-activation domains in such close proximity that they reconstitute the function of the transcription activator, turning ____ the expression of a reporter gene as a result.

A.
B. physically interact, on
C. do not interact, on
Answer» C. do not interact, on
Discussion
14.

Which of the following is untrue regarding the classic yeast two-hybrid method?

A. It is used for the detection of Protein interactions
B. Method that relies on the interaction of “bait” and “prey” proteins in molecular constructs in yeast
C. DNA-binding domain and a trans-activation domain don’t necessarily interact
D. In this strategy, a two-domain transcriptional activator is employed as a helper for determining protein–protein interactions
Answer» D. In this strategy, a two-domain transcriptional activator is employed as a helper for determining protein‚Äö√Ñ√∂‚àö√ë‚àö¬®protein interactions
Discussion