MCQOPTIONS
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MCQOPTIONS
This section includes 14 Mcqs, each offering curated multiple-choice questions to sharpen your Bioinformatics knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which of the following is untrue regarding the STRING? |
| A. | Search Tool for the Retrieval of Interacting Genes/Proteins |
| B. | Functional associations include only the direct protein-protein interactions |
| C. | It is based on combined evidence of gene linkage, gene fusion and phylogenetic profiles |
| D. | It is a web server that predicts gene and protein functional associations |
| Answer» C. It is based on combined evidence of gene linkage, gene fusion and phylogenetic profiles | |
| 2. |
Which of the following is untrue regarding the predicting Interactions Based on Phylogenetic Information? |
| A. | Proteins do not operate as a complex |
| B. | This method detects the co-presence or co-absence of orthologs across a number of genomes |
| C. | Protein interactions can be predicted using phylogenetic profiles |
| D. | Phylogenetic profile are defined as patterns of gene pairs that are concurrently present or absent across genomes |
| Answer» B. This method detects the co-presence or co-absence of orthologs across a number of genomes | |
| 3. |
In Predicting Interactions Based on Gene Neighbors if a certain gene linkage is found to be indeed conserved across divergent genomes, it can be used as a strong indicator of formation of an operon that encodes proteins that are functionally and even physically coupled. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 4. |
Which of the following is untrue regarding the Predicting Interactions Based on Domain Fusion? |
| A. | It is based on gene fusion events |
| B. | Predicting protein–protein interactions is called the “Rosetta stone” method |
| C. | A fused protein often reveals relationships between its domain components |
| D. | A fused protein doesn’t necessarily reveal about the relationships between its domain components |
| Answer» E. | |
| 5. |
An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 6. |
If the bait and prey proteins _______ they bring the DNA-binding and trans-activation domains in such close proximity that they reconstitute the function of the transcription activator, turning ____ the expression of a reporter gene as a result. Which of the following is not the correct pair of blanks? |
| A. | physically interact, on |
| B. | do not interact, on |
| C. | do not interact, off |
| D. | stop interacting, off |
| Answer» C. do not interact, off | |
| 7. |
WHICH_OF_THE_FOLLOWING_IS_UNTRUE_REGARDING_THE_PREDICTING_INTERACTIONS_BASED_ON_PHYLOGENETIC_INFORMATION??$ |
| A. | Proteins do not operate as a complex |
| B. | This method detects the co-presence or co-absence of orthologs across a number of genomes |
| C. | Protein interactions can be predicted using phylogenetic profiles |
| D. | Phylogenetic profile are defined as patterns of gene pairs that are concurrently present or absent across genomes |
| Answer» B. This method detects the co-presence or co-absence of orthologs across a number of genomes | |
| 8. |
Which_of_the_following_is_untrue_regarding_the_STRING?$ |
| A. | Search Tool for the Retrieval of Interacting Genes/Proteins |
| B. | Functional associations include only the direct protein-protein interactions |
| C. | It is based on combined evidence of gene linkage, gene fusion and phylogenetic profiles |
| D. | It is a web server that predicts gene and protein functional associations |
| Answer» C. It is based on combined evidence of gene linkage, gene fusion and phylogenetic profiles | |
| 9. |
In Predicting Interactions Based on Gene Neighbors– if a certain gene linkage is found to be indeed conserved across divergent genomes, it can be used as a strong indicator of formation of an operon that encodes proteins that are functionally and even physically coupled?# |
| A. | True |
| B. | False |
| Answer» B. False | |
| 10. |
The justification behind Rosetta stone method is that when two domains are fused in a single protein, they have to be in _______ proximity to perform a common function. |
| A. | distant |
| B. | close |
| C. | extremely distant |
| D. | extremely close |
| Answer» E. | |
| 11. |
When the two domains are located in two different proteins, to preserve the same functionality, their close proximity and interaction have to be preserved as well. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 12. |
An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column.$ |
| A. | True |
| B. | False |
| Answer» B. False | |
| 13. |
If the bait and prey proteins _______ they bring the DNA-binding and trans-activation domains in such close proximity that they reconstitute the function of the transcription activator, turning ____ the expression of a reporter gene as a result. |
| A. | |
| B. | physically interact, on |
| C. | do not interact, on |
| Answer» C. do not interact, on | |
| 14. |
Which of the following is untrue regarding the classic yeast two-hybrid method? |
| A. | It is used for the detection of Protein interactions |
| B. | Method that relies on the interaction of “bait” and “prey” proteins in molecular constructs in yeast |
| C. | DNA-binding domain and a trans-activation domain don’t necessarily interact |
| D. | In this strategy, a two-domain transcriptional activator is employed as a helper for determining protein–protein interactions |
| Answer» D. In this strategy, a two-domain transcriptional activator is employed as a helper for determining protein‚Äö√Ñ√∂‚àö√ë‚àö¬®protein interactions | |