MCQOPTIONS
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MCQOPTIONS
This section includes 16 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
What is the property of inosine? |
| A. | Having narrow range of pairing capabilities |
| B. | Having a broad range of pairing capabilities |
| C. | The pairing capability is the same as the normal nucleotides |
| D. | It is abbreviated as A |
| Answer» C. The pairing capability is the same as the normal nucleotides | |
| 2. |
In the case of uncertainty, if more than one nucleotide is included at a position it is called ____________ |
| A. | mixed site |
| B. | polynucleotide site |
| C. | unique site |
| D. | degenerate site |
| Answer» B. polynucleotide site | |
| 3. |
Both the primers, the start primer and the end primer should have a nearly same melting temperature. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 4. |
Melting temperature is given by ____________ |
| A. | 4(G+C) + 2(A+T) |
| B. | 2(G+C) + 4(A+T) |
| C. | 2(A+G) + 4(C+T) |
| D. | 4(A+G) + 2(C+T) |
| Answer» B. 2(G+C) + 4(A+T) | |
| 5. |
Which of the following nucleotides should be there at 3’ end? |
| A. | Any of A, T, G or C will work out |
| B. | Either A or T |
| C. | Either G or C |
| D. | Specifically G |
| Answer» D. Specifically G | |
| 6. |
Primers are generally ____________ |
| A. | 20-30 nucleotides long |
| B. | 40-50 nucleotides long |
| C. | as long as the template is |
| D. | taken according to the amount available |
| Answer» B. 40-50 nucleotides long | |
| 7. |
WHAT_IS_THE_PROPERTY_OF_INOSINE??$ |
| A. | Having narrow range of pairing capabilities |
| B. | Having a broad range of pairing capabilities |
| C. | The pairing capability is same as the normal nucleotides |
| D. | It is abbreviated as A |
| Answer» C. The pairing capability is same as the normal nucleotides | |
| 8. |
IN_THE_CASE_OF_UNCERTAINTY,_IF_MORE_THAN_ONE_NUCLEOTIDE_IS_INCLUDED_AT_A_POSITION_IT_IS_CALLED_AS:?$ |
| A. | mixed site |
| B. | polynucleotide site |
| C. | unique site |
| D. | degenerate site |
| Answer» B. polynucleotide site | |
| 9. |
What will happen if the amino acid sequence is used directly for primer designing? |
| A. | There would be certainty because the genetic code is unique for each amino acid |
| B. | There would be uncertainty as the genetic code is degenerate and none of the amino acid is having a unique code |
| C. | There would be uncertainty as the genetic code is degenerate but some of the amino acids such as methionine are having a unique codon |
| D. | The amount of uncertainty or certainty is a matter of chance |
| Answer» D. The amount of uncertainty or certainty is a matter of chance | |
| 10. |
Which of the following is favoured for primer design? |
| A. | The melting temperature should be different for both the primers |
| B. | Primers should be long in length |
| C. | Primers should not be complementary to each other |
| D. | Matching should be of whole primer to the template |
| Answer» D. Matching should be of whole primer to the template | |
| 11. |
Why internal secondary structures are not preferred for primers? |
| A. | Internal structures are very bulky and thus elongation is not preferred |
| B. | Because of it, primer may fold back on itself and won’t be available for template |
| C. | Internal secondary structures require more amount of template |
| D. | If internal structures are present, no proof reading would be observed |
| Answer» C. Internal secondary structures require more amount of template | |
| 12. |
Both the primers, the start primer and the end primer should have nearly same melting temperature. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 13. |
Melting temperature is given by: |
| A. | 4(G+C) + 2(A+T) |
| B. | 2(G+C) + 4(A+T) |
| C. | 2(A+G) + 4(C+T) |
| D. | 4(A+G) + 2(C+T) |
| Answer» B. 2(G+C) + 4(A+T) | |
| 14. |
Which of the following nucleotides should be there at 3’ end?$ |
| A. | Any of A, T, G or C will work out |
| B. | Either A or T |
| C. | Either G or C |
| D. | Specifically G |
| Answer» D. Specifically G | |
| 15. |
Which end of the primer should be matched properly in order to carry out the amplification? |
| A. | 5’ end |
| B. | 3’ end |
| C. | Both of the ends should be matched properly |
| D. | Any one of the ends should match |
| Answer» C. Both of the ends should be matched properly | |
| 16. |
Primers are generally: |
| A. | 20-30 nucleotides long |
| B. | 40-50 nucleotides long |
| C. | as long as the template is |
| D. | taken according to the amount available |
| Answer» B. 40-50 nucleotides long | |