MCQOPTIONS
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This section includes 11 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Species which can grow on _______ as a carbon source are called as methylotropic. |
| A. | methylase |
| B. | methanol |
| C. | methane |
| D. | methanoic acid |
| Answer» C. methane | |
| 2. |
The promoter from fbp1+ gene is suppressed in the presence of glucose. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 3. |
nmt1+ gene is used for _____ biosynthesis. |
| A. | uracil |
| B. | adenine |
| C. | cytosine |
| D. | thiamine |
| Answer» E. | |
| 4. |
Promoters can be divided basically into how many types? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 5. |
The replacement of one plasmid by another by introduction of the second plasmid into strain containing the first, followed by removal of the first by counterselection is known as __________ |
| A. | shuffle vector |
| B. | plasmid shuffle |
| C. | counterselection plasmid |
| D. | combination plasmid |
| Answer» C. counterselection plasmid | |
| 6. |
The leu1+ marker encodes which enzyme? |
| A. | orotidine-5 -phosphate decarboxylase |
| B. | beta isopropylmalate dehydrogenase |
| C. | orotidine-5 -phosphate decarboxylase or beta isopropylmalate dehydrogenase |
| D. | orotidine-5 -phosphate decarboxylase & beta isopropylmalate dehydrogenase |
| Answer» C. orotidine-5 -phosphate decarboxylase or beta isopropylmalate dehydrogenase | |
| 7. |
Schizosaccharomyces pombe is yeast and it reproduces by __________ |
| A. | budding |
| B. | fissing |
| C. | either budding or fissing |
| D. | a method of reproduction other than budding or fissing |
| Answer» C. either budding or fissing | |
| 8. |
Protoplast transformation is less time consuming than lithium acetate for transformation. |
| A. | True |
| B. | False |
| Answer» C. | |
| 9. |
For introduction of exogenous DNA into S.cerevisiae ______ is added along with polyethylene glycol and heat shock treatment. |
| A. | calcium chloride |
| B. | sodium acetate |
| C. | lithium acetate |
| D. | magnesium chloride |
| Answer» D. magnesium chloride | |
| 10. |
The enzyme used for breaking down of sucrose into glucose and fructose is called as _______________ |
| A. | sucrase |
| B. | glucanase |
| C. | maltase |
| D. | invertase |
| Answer» E. | |
| 11. |
Secretion of overexpressed proteins by fusion to a secretion signal can be useful because it helps in bringing about correct _______ and _____ protein folding. |
| A. | glycosylation, enhance |
| B. | glycosylation, not affect |
| C. | phosphorylation, enhance |
| D. | phosphorylation, not affect |
| Answer» B. glycosylation, not affect | |