Explore topic-wise MCQs in Genetic Engineering.

This section includes 6 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

It is easier to subclone a restriction fragment if it belongs to?

A. small gene
B. large gene
C. prokaryotic organism
D. eukaryotic organism
Answer» C. prokaryotic organism
Discussion
2.

If mixed oligonucleotides are used, it is regarded as ______________

A. mixed mutagenesis
B. multiple mutagenesis
C. cassette mutagenesis
D. polymutagenesis
Answer» D. polymutagenesis
Discussion
3.

Several different mutations can be induced at one site.

A. True
B. False
Answer» B. False
Discussion
4.

Once the double stranded molecule with the mutation is introduced into E. coli for replication, how many types of molecules are produced?

A. 1
B. 2
C. 3
D. 4
Answer» C. 3
Discussion
5.

Which of the following statement is incorrect for the synthesis of the second strand?

A. The oligonucleotide is acting as a primer for the synthesis of the second strand
B. DNA polymerase and dNTPs are added for synthesis
C. The polymerase should have 5 -3 exonuclease activity
D. A polymerase having 5 -3 exonuclease activity would degrade the primer that carries the mutant sequence
Answer» D. A polymerase having 5 -3 exonuclease activity would degrade the primer that carries the mutant sequence
Discussion
6.

If the template is double stranded, they need to be separated before annealing of oligonucleotide.

A. True
B. False
Answer» B. False
Discussion