Emulsion or droplet PCR is another modification of PCR. Which of the statement holds true?

It refers to the PCR carried out at large scale

It is possible to incorporate the reagents in a lipid drop

It refers to the PCR carried out at large scale

The temperature variation is not possible easily

If there is a single template molecule at the start then amplification results in the mixture and it because of the extent of extension carried out

Which of the statement is incorrect for anchored PCR?

Anchored PCR is the modification in which only one piece of the sequence is known and thus one primer

The known sequence is attached to the required region of amplification and then further used as a second priming site

Fragment the sample DNA and ligate it to known sequence

Tails are added enzymatically to the region of known sequence

Which one of the following is not done if amplification of the non flanking region is carried out?

Firstly, restriction enzyme digestion is done of those sites whose sequence is not known

Then the self-ligation of molecules is allowed

Now the molecules are cleaved where the known sequence is

Again, the molecules are linearized and the known sequence is in the middle

Explore topic-wise MCQs in Genetic Engineering.

This section includes 6 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.	Emulsion or droplet PCR is another modification of PCR. Which of the statement holds true?
A.	It is possible to incorporate the reagents in a lipid drop
B.	It refers to the PCR carried out at large scale
C.	The temperature variation is not possible easily
D.	If there is a single template molecule at the start then amplification results in the mixture and it because of the extent of extension carried out
Answer» B. It refers to the PCR carried out at large scale

Discussion

2.	Which of the statement is incorrect for anchored PCR?
A.	Anchored PCR is the modification in which only one piece of the sequence is known and thus one primer
B.	The known sequence is attached to the required region of amplification and then further used as a second priming site
C.	Fragment the sample DNA and ligate it to known sequence
D.	Tails are added enzymatically to the region of known sequence
Answer» E.

Discussion

3.	If amplification of one of the strand is favoured, the modification of PCR is known as __________
A.	single-strand PCR
B.	partial PCR
C.	asymmetric PCR
D.	anchored PCR
Answer» D. anchored PCR

Discussion

4.	Mutation is never deliberately induced in PCR products.
A.	True
B.	False
Answer» C.

Discussion

5.	By using two oligonucleotides, we can measure the amount of quantity of DNA by measuring the amount of fluorescence. The first probe absorbs light and the second emits it at a particular wavelength.
A.	True
B.	False
Answer» B. False

Discussion

6.	Which one of the following is not done if amplification of the non flanking region is carried out?
A.	Firstly, restriction enzyme digestion is done of those sites whose sequence is not known
B.	Then the self-ligation of molecules is allowed
C.	Now the molecules are cleaved where the known sequence is
D.	Again, the molecules are linearized and the known sequence is in the middle
Answer» E.

Discussion

Explore topic-wise MCQs in Genetic Engineering.

Emulsion or droplet PCR is another modification of PCR. Which of the statement holds true?

Which of the statement is incorrect for anchored PCR?

If amplification of one of the strand is favoured, the modification of PCR is known as __________

Mutation is never deliberately induced in PCR products.

By using two oligonucleotides, we can measure the amount of quantity of DNA by measuring the amount of fluorescence. The first probe absorbs light and the second emits it at a particular wavelength.

Which one of the following is not done if amplification of the non flanking region is carried out?