MCQOPTIONS
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MCQOPTIONS
This section includes 8 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
If linkers are combined with other features such as a selectable marker, it is called as __________ |
| A. | cassette |
| B. | modified linker |
| C. | adaptors |
| D. | induced linker |
| Answer» B. modified linker | |
| 2. |
There is no effect if the insert itself contains the restriction site. |
| A. | True |
| B. | False |
| Answer» C. | |
| 3. |
Linkers are often used in cloning. Choose the incorrect statement for linkers. |
| A. | These are short chemically synthesized molecules that contain a particular restriction enzyme site within the sequence |
| B. | They are blunt ended molecules |
| C. | They are ligated to staggered ended insert molecules by T4 DNA ligase |
| D. | After treatment with enzyme, both the ends of the linker are staggered |
| Answer» D. After treatment with enzyme, both the ends of the linker are staggered | |
| 4. |
Choose the incorrect statement in respect to topoisomerase I. |
| A. | It is used to cleave only one DNA strand |
| B. | It leaves at the recognition sequence C/TCCTT- and is covalently attached to one end of the cleaved molecule |
| C. | The supercoiling of the DNA strand is altered and then it is sealed again by the enzyme |
| D. | It is slower than the normal DNA ligase |
| Answer» E. | |
| 5. |
TA cloning is a method used for cloning of PCR products. Which of the statement is correct with respect to it? |
| A. | It is based on the fact that a T residue is incorporated at the end of the PCR product |
| B. | A residue is incorporated into the end of the vector |
| C. | It gives a low yield |
| D. | It is preferred over blunt end ligation |
| Answer» E. | |
| 6. |
When inserting a DNA fragment, it is possible to have two orientations. If the orientation is controlled, this cloning is referred to as __________ |
| A. | forced cloning |
| B. | orientation cloning |
| C. | correct cloning |
| D. | restricted cloning |
| Answer» B. orientation cloning | |
| 7. |
ccdB gene is used at times. Choose the correct statement with respect to this gene. |
| A. | It is a control of cell birth gene |
| B. | It is activated in complex vectors to increase the fraction of recombinants |
| C. | If intramolecular ligation takes place this protein is released |
| D. | It is often regarded as control of cell death and birth gene |
| Answer» C. If intramolecular ligation takes place this protein is released | |
| 8. |
There are various methods to distinguish whether a colony contains a recombinant or not. One such method is __________ |
| A. | blue white screening |
| B. | checking whether replication is taking place or not |
| C. | checking the number of copies |
| D. | looking for the multiple cloning site |
| Answer» B. checking whether replication is taking place or not | |