MCQOPTIONS
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MCQOPTIONS
This section includes 16 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
If colEI derived origin of replication is replaced by the origin of replication of F plasmid, it is called as: |
| A. | phagemid |
| B. | F cosmid |
| C. | plasmid |
| D. | fosmid |
| Answer» E. | |
| 2. |
What happens once the cosmid enters the E. coli cells? |
| A. | There is strict size selection inside E. coli cells |
| B. | Partial deletion may take place |
| C. | The tendency of deletion may is increased by using low copy number |
| D. | The tendency of deletion can’t be altered |
| Answer» C. The tendency of deletion may is increased by using low copy number | |
| 3. |
Which size of the insert is accepted by the cosmids? |
| A. | 10-20 kbp |
| B. | 35-45 kbp |
| C. | 50-60 kbp |
| D. | 100-120 kbp |
| Answer» C. 50-60 kbp | |
| 4. |
Once cosmids are inside the E.coli cells, they don’t generate phage but are propagated as plasmids. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 5. |
Which of the following doesn’t takes place after replication? |
| A. | The single stranded sequence is generated |
| B. | It is circularized to form closed single stranded molecule |
| C. | It may circularize or remain linear |
| D. | The double stranded molecule can be synthesized by cellular DNA synthesis |
| Answer» D. The double stranded molecule can be synthesized by cellular DNA synthesis | |
| 6. |
Choose the correct statement for the lambda ZAP vector. |
| A. | It is not based on bacteriophage lambda |
| B. | It contains a region that can be excised in vivo |
| C. | The excision leads to the formation of bluescript plasmids and it contains an initiator region only |
| D. | The multiple cloning site is not flanked by the initiator and the terminator region |
| Answer» C. The excision leads to the formation of bluescript plasmids and it contains an initiator region only | |
| 7. |
WHAT_HAPPENS_ONCE_THE_COSMID_ENTERS_THE_E._COLI_CELLS??$ |
| A. | There is strict size selection inside E. coli cells |
| B. | Partial deletion may take place |
| C. | The tendency of deletion may is increased by using low copy number |
| D. | The tendency of deletion can’t be altered |
| Answer» C. The tendency of deletion may is increased by using low copy number | |
| 8. |
If_colEI_derived_origin_of_replication_is_replaced_by_origin_of_replication_of_F_plasmid,_it_is_called_as:$ |
| A. | phagemid |
| B. | F cosmid |
| C. | plasmid |
| D. | fosmid |
| Answer» E. | |
| 9. |
Which size of insert is accepted by the cosmids? |
| A. | 10-20 kbp |
| B. | 35-45 kbp |
| C. | 50-60 kbp |
| D. | 100-120 kbp |
| Answer» C. 50-60 kbp | |
| 10. |
Which of the following is the correct method to check whether the DNA has entered into the cell or not in the case of cosmid? |
| A. | If transformation has taken place turbid plaques are formed |
| B. | If transformation has taken place clear plaques are formed |
| C. | If transformation has taken place, it can be confirmed via ampicillin resistance |
| D. | If transformation has taken place, it can be confirmed if forms plaques and is ampicillin resistant also |
| Answer» D. If transformation has taken place, it can be confirmed if forms plaques and is ampicillin resistant also | |
| 11. |
Once cosmids are inside the E.coli cells, they don’t generate phage but are propagated as plasmids. Is the given statement true or false.$ |
| A. | True |
| B. | False |
| Answer» B. False | |
| 12. |
Choose the correct statement for cosmids. |
| A. | It can be regarded as lambda substitution vector |
| B. | Less amount of phage DNA is deleted |
| C. | Only cos packaging sites are left |
| D. | It doesn’t contains a origin of replication |
| Answer» D. It doesn‚Äö√Ñ√∂‚àö√ë‚àö¬•t contains a origin of replication | |
| 13. |
Which of the following doesn’t takes place after replication?$ |
| A. | The single stranded sequence is generated |
| B. | It is circularized to form closed single stranded molecule |
| C. | It may circularize or remain linear |
| D. | The double stranded molecule can be synthesized by cellular DNA synthesis |
| Answer» D. The double stranded molecule can be synthesized by cellular DNA synthesis | |
| 14. |
Choose the incorrect statement for the replication process. |
| A. | The initiator site is nicked and replication of one strand is started |
| B. | Replication takes place in both the directions |
| C. | Replication continues through the bluescript |
| D. | It is stopped at the terminator and then again a nick is made |
| Answer» C. Replication continues through the bluescript | |
| 15. |
The initiator is recognized by which gene? |
| A. | Gene I |
| B. | Gene I and II |
| C. | Only gene II |
| D. | Gene III |
| Answer» D. Gene III | |
| 16. |
Choose the correct statement for lambda ZAP vector. |
| A. | It is not based on bacteriophage lambda |
| B. | It contains a region that can be excised in vivo |
| C. | The excision leads to the formation of bluescript plasmids and it contains an initiator region only |
| D. | The multiple cloning site is not flanked by the initiator and the terminator region |
| Answer» C. The excision leads to the formation of bluescript plasmids and it contains an initiator region only | |