Which of the following is untrue about DNA sequencing?

This involves the synthesis of DNA chains of varying length

It is now routinely carried out using the Sanger method

This doesn’t make use of DNA polymerases

This involves the synthesis of DNA chains of varying length

The DNA synthesis is stopped by adding dideoxynucleotides

Which of the following is untrue about Gene Order Comparison?

Generally, gene order is much less conserved compared with gene sequences

When the order of a number of linked genes is conserved between genomes, it is called synteny

Generally, gene order is much more conserved compared with gene sequences

Generally, gene order is much less conserved compared with gene sequences

It is in fact rarely observed among divergent species

Which of the following is an incorrect statement about the terminologies related to protein sorting?

Protein sorting is also known as protein targeting

Subcellular localization is an integral part of protein functionality

Many proteins exhibit functions only after being transported to certain compartments of the cell

All the proteins exhibit functions after being transported to certain compartments of the cell

Protein sorting is also known as protein targeting

Which of the following is a wrong statement?

To assume biological activity, many nascent polypeptides have to be covalently modified before or after the folding process

In eukaryotic cells most modifications take place in the endoplasmic reticulum and the Golgi apparatus

The modifications in eukaryotic cells include proteolytic cleavage; formation of disulfide bonds; addition of phosphoryl, methyl, acetyl, or other groups onto certain amino acid residues

The modifications in eukaryotic cells doesn’t include attachment of oligosaccharides or prosthetic groups to create mature proteins

Which of the following is untrue about SAGE Genie?

It is an NCBI web-based program

It allows matching of experimentally obtained SAGE tags to known genes

It provides an interface for visualizing human gene expression

It doesn’t filter out linker sequences from experimentally obtained SAGE tags

Which of the following is untrue about SAGE?

Here, sequencing is economical but time-consuming step

Sequencing is the most costly and time-consuming step

Here, sequencing is economical but time-consuming step

Sequencing is economical but time-reducing step

It is difficult to know how many tags need to be sequenced to get a good coverage of the entire transcriptome

Which of the following is incorrect regarding 2D-Page?

One of the challenges of this technique is the separation of membrane proteins

Not all proteins can be separated by this method or stained properly

The stained gel can be scanned and digitized for image analysis

Membrane proteins are largely hydrophilic and readily solubilized

One of the challenges of this technique is the separation of membrane proteins

Which of the following is untrue about the drawbacks of SAGE?

The drawback with this approach is the sensitivity to sequencing errors

One or two sequencing errors in the tag sequence can lead to ambiguous or erroneous tag identification

Correctly sequenced SAGE tag sometimes may correspond to several genes or no gene at all

Correctly sequenced SAGE tag always corresponds to several genes

The drawback with this approach is the sensitivity to sequencing errors

Which of the following is untrue regarding expressed sequence tags (ESTs)?

One of the high throughput approaches to genome-wide profiling of gene expression is sequencing ESTs

They are short sequences obtained from cDNA clones

They serve as short identifiers of full-length genes

They are typically in the range of 800 to 900 nucleotides in length

Which of the following is incorrect about Self-Organizing Maps?

The data points are initially assigned to the nodes at random

Clustering by SOMs is in principle similar to the k-means method

It doesn’t involve neural networks

The data points are initially assigned to the nodes at random

It starts by defining a number of nodes

Which of the following is untrue regarding UniGene?

It is an NCBI EST cluster database

Overlapping EST sequences are computationally processed to represent a single expressed gene

Each cluster is a set of overlapping EST sequences

The overlapping EST sequences are computationally processed to represent a set of expressed genes

Which of the following is a wrong about AutoMotif?

In this process, the query sequence is chopped up into a number of overlapping fragments

It is a web server predicting protein sequence motifs

In this process, the query sequence is chopped up into a number of overlapping fragments

The overlapping fragments from are query sequence are fed into different kernels (similar to nodes)

Which of the following is incorrect about Classification of microarray data?

For microarray data, clustering analysis identifies coexpressed but not coregulated genes

For microarray data, clustering analysis identifies coexpressed and coregulated genes

For microarray data, clustering analysis identifies coexpressed but not coregulated genes

For microarray data, clustering analysis identifies and coregulated but not coexpressed genes

Genes within a category have more similarity in expression than genes from different categories.

Which of the following is untrue about LAGAN?

It first finds anchors between two genomic sequences using an algorithm that identifies short, exactly matching words

It stands for Limited Area Global Alignment of Nucleotides

It is a web-based program designed for pairwise alignment of small fragments of genomes only

It first finds anchors between two genomic sequences using an algorithm that identifies short, exactly matching words

Regions that have high density of words are selected as anchors

Which of the following is untrue regarding the classic yeast two-hybrid method?

In this strategy, a two-domain transcriptional activator is employed as a helper for determining protein–protein interactions

It is used for the detection of Protein interactions

Method that relies on the interaction of “bait” and “prey” proteins in molecular constructs in yeast

DNA-binding domain and a trans-activation domain don’t necessarily interact

In this strategy, a two-domain transcriptional activator is employed as a helper for determining protein–protein interactions

Which of the following is incorrect about a microarray?

Each oligomer is spotted on the slide and serves as a probe for binding to a unique complementary cDNA

It is a slide attached with a high-density array of immobilized DNA oligomers representing the entire genome of the species under study

Array of immobilized DNA oligomers cannot be cDNAs

Each oligomer is spotted on the slide and serves as a probe for binding to a unique complementary cDNA

It is the most commonly used global gene expression profiling method

The signal sequences are typically _______ residues long, rich in _____ charged residues such as arginines as well as hydroxyl residues such as serines and threonines, but devoid of ______ charged residues.

300 to 800, negatively, positively

28 to 80, positively, negatively

300 to 800, negatively, positively

28 to 80, negatively, positively

300 to 500, positively, negatively

The hierarchical genome sequencing approach is ______

entirely dissimilar to the shotgun approach

dissimilar to the shotgun approach

similar to the shotgun approach, but on a larger scale

similar to the shotgun approach, but on a smaller scale

Which of the following is incorrect about EULER?

The tuples are distributed in a diagram with numerous nodes that are all interconnected

It uses a Eulerian Superpath approach, which is a polynomial algorithm

In this approach, a sequence fragment is broken down to tuples of five nucleotides

The tuples are distributed in a diagram with numerous nodes that are all interconnected

Which of the following is incorrect about Phrap?

It is used for sequence assembly

It aligns individual fragments in a pairwise fashion using the Smith–Waterman algorithm

It doesn’t take input from Phred

It is used for sequence assembly

Which of the following is incorrect about ARACHNE?

It accepts base calls with associated quality scores assigned by Phred as input

It is for the assembly of whole-genome shotgun reads

It doesn’t involve heuristic approach

When a sequence is generated from ____ ends of a single clone, the distance between the two opposing fragments of a clone is fixed to ________ meaning that they are always separated by a distance defined by a _____ length (normally 1,000 to 9,000 bases).

both, an uncertain range, clone

Which of the following is incorrect regarding Automated Genome Annotation?

Automation brings speed in gene annotation process

It exists because of the need to develop fast and automated methods to annotate the genomic sequences

The automated approach relies on homology detection

The automated approach doesn’t rely on heuristic sequence similarity searching

Automation brings speed in gene annotation process

Which of the following is incorrect about Phred?

It doesn’t give a probability score in output

It uses a Fourier analysis to resolve fluorescence traces and predict actual peak locations of bases

Which of the following is incorrect regarding GeneQuiz?

It is a web server for protein sequence annotation

It is a web server for protein DNA annotation

It is a web server for protein sequence annotation

It compares a query sequence against databases using BLAST and FASTA to identify homologs with high similarities

It performs domain analysis using the PROSITE and Blocks databases

Which of the following is incorrect regarding genome economy?

This is a major strategy that eukaryotic organisms use to achieve a myriad of phenotypic diversities

It is a phenomenon of synthesizing more proteins from fewer genes

This is a major strategy that eukaryotic organisms use to achieve a myriad of genotypic diversities only

This is a major strategy that eukaryotic organisms use to achieve a myriad of phenotypic diversities

There are numerous underlying genetic mechanisms to help account for genome economy

Which of the following is incorrect regarding gene annotation?

The gene annotation of the human genome employs a combination of theoretical prediction and experimental verification

Gene structures are first predicted by ab initio exon prediction programs

The predicted genes are compared with experimentally determined cDNA and EST sequences

The pairwise alignment programs are not involved

113 + Mcqs in Genomics and Proteomics in Bioinformatics Page 2 McqOptions

51.	DNA microarrays measure “absolute” mRNA expression levels without arbitrary reference standards, whereas SAGE indicates the relative expression levels.
A.	True
B.	False
C.	May be True or False
D.	Can't say
Answer» C. May be True or False

Discussion

52.	The _____ resolution genome map is the genomic DNA sequence that can be considered as a type of ______ map describing a genome at the single base-pair level.
A.	highest, physical
B.	lowest, physical
C.	highest, cytological
D.	lowest, cytological
Answer» B. lowest, physical

Discussion

53.	Which of the following is untrue about DNA sequencing?
A.	It is now routinely carried out using the Sanger method
B.	This doesn’t make use of DNA polymerases
C.	This involves the synthesis of DNA chains of varying length
D.	The DNA synthesis is stopped by adding dideoxynucleotides
Answer» C. This involves the synthesis of DNA chains of varying length

Discussion

54.	In DNA sequencing, the fluorescent traces of the DNA sequences are read by a computer program that assigns bases for each peak in a chromatogram.
A.	True
B.	False
C.	May be True or False
D.	Can't say
Answer» B. False

Discussion

55.	The shotgun approach _______ sequences clones from _____ of cloned DNA.
A.	randomly, one end
B.	randomly, both ends
C.	specifically, both ends
D.	specifically, one end
Answer» C. specifically, both ends

Discussion

56.	Which of the following is untrue about Gene Order Comparison?
A.	When the order of a number of linked genes is conserved between genomes, it is called synteny
B.	Generally, gene order is much more conserved compared with gene sequences
C.	Generally, gene order is much less conserved compared with gene sequences
D.	It is in fact rarely observed among divergent species
Answer» C. Generally, gene order is much less conserved compared with gene sequences

Discussion

57.	Which of the following is an incorrect statement about the terminologies related to protein sorting?
A.	Subcellular localization is an integral part of protein functionality
B.	Many proteins exhibit functions only after being transported to certain compartments of the cell
C.	All the proteins exhibit functions after being transported to certain compartments of the cell
D.	Protein sorting is also known as protein targeting
Answer» D. Protein sorting is also known as protein targeting

Discussion

58.	Which of the following is a wrong statement?
A.	To assume biological activity, many nascent polypeptides have to be covalently modified before or after the folding process
B.	In eukaryotic cells most modifications take place in the endoplasmic reticulum and the Golgi apparatus
C.	The modifications in eukaryotic cells include proteolytic cleavage; formation of disulfide bonds; addition of phosphoryl, methyl, acetyl, or other groups onto certain amino acid residues
D.	The modifications in eukaryotic cells doesn’t include attachment of oligosaccharides or prosthetic groups to create mature proteins
Answer» E.

Discussion

59.	Which of the following is untrue about SAGE Genie?
A.	It is an NCBI web-based program
B.	It allows matching of experimentally obtained SAGE tags to known genes
C.	It provides an interface for visualizing human gene expression
D.	It doesn’t filter out linker sequences from experimentally obtained SAGE tags
Answer» E.

Discussion

60.	Which of the following is untrue about SAGE?
A.	Sequencing is the most costly and time-consuming step
B.	Here, sequencing is economical but time-consuming step
C.	Sequencing is economical but time-reducing step
D.	It is difficult to know how many tags need to be sequenced to get a good coverage of the entire transcriptome
Answer» B. Here, sequencing is economical but time-consuming step

Discussion

61.	Which of the following is incorrect regarding 2D-Page?
A.	Not all proteins can be separated by this method or stained properly
B.	The stained gel can be scanned and digitized for image analysis
C.	Membrane proteins are largely hydrophilic and readily solubilized
D.	One of the challenges of this technique is the separation of membrane proteins
Answer» D. One of the challenges of this technique is the separation of membrane proteins

Discussion

62.	Which of the following is untrue about the drawbacks of SAGE?
A.	One or two sequencing errors in the tag sequence can lead to ambiguous or erroneous tag identification
B.	Correctly sequenced SAGE tag sometimes may correspond to several genes or no gene at all
C.	Correctly sequenced SAGE tag always corresponds to several genes
D.	The drawback with this approach is the sensitivity to sequencing errors
Answer» D. The drawback with this approach is the sensitivity to sequencing errors

Discussion

63.	Which of the following is untrue regarding expressed sequence tags (ESTs)?
A.	One of the high throughput approaches to genome-wide profiling of gene expression is sequencing ESTs
B.	They are short sequences obtained from cDNA clones
C.	They serve as short identifiers of full-length genes
D.	They are typically in the range of 800 to 900 nucleotides in length
Answer» E.

Discussion

64.	Chloroplast localization signals are also located in the __________ terminus and are about 25 to 100 residues in length, containing very few _______ charged residues but many hydroxylated residues such as serine.
A.	N, negatively
B.	C, negatively
C.	C, positively
D.	N, positively
Answer» B. C, negatively

Discussion

65.	SAGEmap is a SAGE database created by NCBI.
A.	True
B.	False
C.	May be True or False
D.	Can't say
Answer» B. False

Discussion

66.	To generate EST data, clones in the cDNA library are randomly selected for sequencing from either end of the inserts.
A.	True
B.	False
C.	May be True or False
D.	Can't say
Answer» B. False

Discussion

67.	The hierarchical approach is ____ and _____ than the shotgun approach because it involves an initial clone-based physical mapping step.
A.	slower, less costly
B.	faster, more costly
C.	faster, less costly
D.	slower, more costly
Answer» E.

Discussion

68.	An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column.
A.	True
B.	False
C.	May be True or False
D.	Can't say
Answer» B. False

Discussion

69.	In the analysis of microarray data–If replicated datasets are available, rigorous statistical tests such as t-test and analysis of variance (ANOVA) can be performed to test the null hypothesis that a given data point is not significantly different from the mean of the data distribution.
A.	True
B.	False
C.	May be True or False
D.	Can't say
Answer» B. False

Discussion

70.	SAGExProfiler doesn’t provide information about overexpressed or silenced genes.
A.	True
B.	False
C.	May be True or False
D.	Can't say
Answer» C. May be True or False

Discussion

71.	The whole genome shotgun approach can produce a draft sequence very rapidly because it is based on the direct sequencing approach.
A.	True
B.	False
C.	May be True or False
D.	Can't say
Answer» B. False

Discussion

72.	Despite the multiple coverage, sometimes certain genomic regions remain unsequenced, mainly owing to cloning difficulties.
A.	True
B.	False
C.	May be True or False
D.	Can't say
Answer» B. False

Discussion

73.	Which of the following is incorrect about Self-Organizing Maps?
A.	Clustering by SOMs is in principle similar to the k-means method
B.	It doesn’t involve neural networks
C.	The data points are initially assigned to the nodes at random
D.	It starts by defining a number of nodes
Answer» C. The data points are initially assigned to the nodes at random

Discussion

74.	Which of the following is untrue regarding UniGene?
A.	It is an NCBI EST cluster database
B.	Overlapping EST sequences are computationally processed to represent a single expressed gene
C.	Each cluster is a set of overlapping EST sequences
D.	The overlapping EST sequences are computationally processed to represent a set of expressed genes
Answer» E.

Discussion

75.	The classic protein separation methods involve two-dimensional gel electrophoresis followed by gel image analysis.
A.	True
B.	False
C.	May be True or False
D.	Can't say
Answer» B. False

Discussion

76.	Which of the following is a wrong about AutoMotif?
A.	It is a web server predicting protein sequence motifs
B.	It doesn’t use SVM approach
C.	In this process, the query sequence is chopped up into a number of overlapping fragments
D.	The overlapping fragments from are query sequence are fed into different kernels (similar to nodes)
Answer» C. In this process, the query sequence is chopped up into a number of overlapping fragments

Discussion

77.	Which of the following is not one of the training sets in SignalP?
A.	Prokaryotes
B.	Eukaryotes
C.	Gram-positive bacteria
D.	Gram-negative bacteria
Answer» B. Eukaryotes

Discussion

78.	Which of the following is incorrect about Classification of microarray data?
A.	For microarray data, clustering analysis identifies coexpressed and coregulated genes
B.	For microarray data, clustering analysis identifies coexpressed but not coregulated genes
C.	For microarray data, clustering analysis identifies and coregulated but not coexpressed genes
D.	Genes within a category have more similarity in expression than genes from different categories.
Answer» B. For microarray data, clustering analysis identifies coexpressed but not coregulated genes

Discussion

79.	Which of the following is untrue about LAGAN?
A.	It stands for Limited Area Global Alignment of Nucleotides
B.	It is a web-based program designed for pairwise alignment of small fragments of genomes only
C.	It first finds anchors between two genomic sequences using an algorithm that identifies short, exactly matching words
D.	Regions that have high density of words are selected as anchors
Answer» C. It first finds anchors between two genomic sequences using an algorithm that identifies short, exactly matching words

Discussion

80.	A supervised analysis refers to classification of data into a set of predefined categories. For example, depending on the purpose of the experiment, the data can be classified into predefined ‘diseased’ or ‘normal’ categories.
A.	True
B.	False
C.	May be True or False
D.	Can't say
Answer» B. False

Discussion

81.	Which of the following is untrue regarding the classic yeast two-hybrid method?
A.	It is used for the detection of Protein interactions
B.	Method that relies on the interaction of “bait” and “prey” proteins in molecular constructs in yeast
C.	DNA-binding domain and a trans-activation domain don’t necessarily interact
D.	In this strategy, a two-domain transcriptional activator is employed as a helper for determining protein–protein interactions
Answer» D. In this strategy, a two-domain transcriptional activator is employed as a helper for determining protein–protein interactions

Discussion

82.	Physical maps are constructed by using a chromosome walking technique.
A.	True
B.	False
C.	May be True or False
D.	Can't say
Answer» B. False

Discussion

83.	Which of the following is incorrect about a microarray?
A.	It is a slide attached with a high-density array of immobilized DNA oligomers representing the entire genome of the species under study
B.	Array of immobilized DNA oligomers cannot be cDNAs
C.	Each oligomer is spotted on the slide and serves as a probe for binding to a unique complementary cDNA
D.	It is the most commonly used global gene expression profiling method
Answer» C. Each oligomer is spotted on the slide and serves as a probe for binding to a unique complementary cDNA

Discussion

84.	The signal sequences are typically _______ residues long, rich in _____ charged residues such as arginines as well as hydroxyl residues such as serines and threonines, but devoid of ______ charged residues.
A.	28 to 80, positively, negatively
B.	300 to 800, negatively, positively
C.	28 to 80, negatively, positively
D.	300 to 500, positively, negatively
Answer» B. 300 to 800, negatively, positively

Discussion

85.	The hierarchical genome sequencing approach is ______
A.	entirely dissimilar to the shotgun approach
B.	dissimilar to the shotgun approach
C.	similar to the shotgun approach, but on a larger scale
D.	similar to the shotgun approach, but on a smaller scale
Answer» E.

Discussion

86.	Which of the following is incorrect about EULER?
A.	It is an assembly algorithm
B.	It uses a Eulerian Superpath approach, which is a polynomial algorithm
C.	In this approach, a sequence fragment is broken down to tuples of five nucleotides
D.	The tuples are distributed in a diagram with numerous nodes that are all interconnected
Answer» D. The tuples are distributed in a diagram with numerous nodes that are all interconnected

Discussion

87.	TIGR Assembler is a UNIX program from TIGR for assembly of large shotgun sequence fragments.
A.	True
B.	False
C.	May be True or False
D.	Can't say
Answer» B. False

Discussion

88.	Which of the following is incorrect about Phrap?
A.	It aligns individual fragments in a pairwise fashion using the Smith–Waterman algorithm
B.	It doesn’t take input from Phred
C.	It is used for sequence assembly
D.	It is a UNIX program
Answer» C. It is used for sequence assembly

Discussion

89.	Which of the following is incorrect about ARACHNE?
A.	It accepts base calls with associated quality scores assigned by Phred as input
B.	It is a free UNIX program
C.	It is for the assembly of whole-genome shotgun reads
D.	It doesn’t involve heuristic approach
Answer» E.

Discussion

90.	VecScreen is a primarily aimed for sequence assembly.
A.	True
B.	False
C.	May be True or False
D.	Can't say
Answer» C. May be True or False

Discussion

91.	When a sequence is generated from ____ ends of a single clone, the distance between the two opposing fragments of a clone is fixed to ________ meaning that they are always separated by a distance defined by a _____ length (normally 1,000 to 9,000 bases).
A.	both, an uncertain range, clone
B.	one, an uncertain range, clone
C.	both, a certain range, clone
D.	both, a certain range, gene
Answer» D. both, a certain range, gene

Discussion

92.	The major challenges in genome assembly are sequence errors, contamination by bacterial vectors, and repetitive sequence regions.
A.	True
B.	False
C.	May be True or False
D.	Can't say
Answer» B. False

Discussion

93.	Which of the following is incorrect regarding Automated Genome Annotation?
A.	It exists because of the need to develop fast and automated methods to annotate the genomic sequences
B.	The automated approach relies on homology detection
C.	The automated approach doesn’t rely on heuristic sequence similarity searching
D.	Automation brings speed in gene annotation process
Answer» D. Automation brings speed in gene annotation process

Discussion

94.	Which of the following is incorrect about Phred?
A.	It is a UNIX program
B.	It doesn’t give a probability score in output
C.	It is used for base calling
D.	It uses a Fourier analysis to resolve fluorescence traces and predict actual peak locations of bases
Answer» C. It is used for base calling

Discussion

95.	In some circumstances, one mRNA transcript can lead to the translation of more than one protein.
A.	True
B.	False
C.	May be True or False
D.	Can't say
Answer» B. False

Discussion

96.	Which of the following is incorrect regarding GeneQuiz?
A.	It is a web server for protein DNA annotation
B.	It is a web server for protein sequence annotation
C.	It compares a query sequence against databases using BLAST and FASTA to identify homologs with high similarities
D.	It performs domain analysis using the PROSITE and Blocks databases
Answer» B. It is a web server for protein sequence annotation

Discussion

97.	Which of the following is incorrect regarding genome economy?
A.	It is a phenomenon of synthesizing more proteins from fewer genes
B.	This is a major strategy that eukaryotic organisms use to achieve a myriad of genotypic diversities only
C.	This is a major strategy that eukaryotic organisms use to achieve a myriad of phenotypic diversities
D.	There are numerous underlying genetic mechanisms to help account for genome economy
Answer» C. This is a major strategy that eukaryotic organisms use to achieve a myriad of phenotypic diversities

Discussion

98.	Conserved functional sites can be identified by profile and hidden Markov model–based motif and domain search tools such as SMART and InterPro.
A.	True
B.	False
C.	May be True or False
D.	Can't say
Answer» B. False

Discussion

99.	The remote homology detection helps to shed light on the possible functions of the proteins that previously have no functional information at all.
A.	True
B.	False
C.	May be True or False
D.	Can't say
Answer» B. False

Discussion

100.	Which of the following is incorrect regarding gene annotation?
A.	The gene annotation of the human genome employs a combination of theoretical prediction and experimental verification
B.	Gene structures are first predicted by ab initio exon prediction programs
C.	The predicted genes are compared with experimentally determined cDNA and EST sequences
D.	The pairwise alignment programs are not involved
Answer» E.

Discussion

Explore topic-wise MCQs in Bioinformatics.

DNA microarrays measure “absolute” mRNA expression levels without arbitrary reference standards, whereas SAGE indicates the relative expression levels.

The _____ resolution genome map is the genomic DNA sequence that can be considered as a type of ______ map describing a genome at the single base-pair level.

Which of the following is untrue about DNA sequencing?

In DNA sequencing, the fluorescent traces of the DNA sequences are read by a computer program that assigns bases for each peak in a chromatogram.

The shotgun approach _______ sequences clones from _____ of cloned DNA.

Which of the following is untrue about Gene Order Comparison?

Which of the following is an incorrect statement about the terminologies related to protein sorting?

Which of the following is a wrong statement?

Which of the following is untrue about SAGE Genie?

Which of the following is untrue about SAGE?

Which of the following is incorrect regarding 2D-Page?

Which of the following is untrue about the drawbacks of SAGE?

Which of the following is untrue regarding expressed sequence tags (ESTs)?

Chloroplast localization signals are also located in the __________ terminus and are about 25 to 100 residues in length, containing very few _______ charged residues but many hydroxylated residues such as serine.

SAGEmap is a SAGE database created by NCBI.

To generate EST data, clones in the cDNA library are randomly selected for sequencing from either end of the inserts.

The hierarchical approach is ____ and _____ than the shotgun approach because it involves an initial clone-based physical mapping step.

An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column.

In the analysis of microarray data–If replicated datasets are available, rigorous statistical tests such as t-test and analysis of variance (ANOVA) can be performed to test the null hypothesis that a given data point is not significantly different from the mean of the data distribution.

SAGExProfiler doesn’t provide information about overexpressed or silenced genes.

The whole genome shotgun approach can produce a draft sequence very rapidly because it is based on the direct sequencing approach.

Despite the multiple coverage, sometimes certain genomic regions remain unsequenced, mainly owing to cloning difficulties.

Which of the following is incorrect about Self-Organizing Maps?

Which of the following is untrue regarding UniGene?

The classic protein separation methods involve two-dimensional gel electrophoresis followed by gel image analysis.

Which of the following is a wrong about AutoMotif?

Which of the following is not one of the training sets in SignalP?

Which of the following is incorrect about Classification of microarray data?

Which of the following is untrue about LAGAN?

A supervised analysis refers to classification of data into a set of predefined categories. For example, depending on the purpose of the experiment, the data can be classified into predefined ‘diseased’ or ‘normal’ categories.

Which of the following is untrue regarding the classic yeast two-hybrid method?

Physical maps are constructed by using a chromosome walking technique.

Which of the following is incorrect about a microarray?

The signal sequences are typically _______ residues long, rich in _____ charged residues such as arginines as well as hydroxyl residues such as serines and threonines, but devoid of ______ charged residues.

The hierarchical genome sequencing approach is ______

Which of the following is incorrect about EULER?

TIGR Assembler is a UNIX program from TIGR for assembly of large shotgun sequence fragments.

Which of the following is incorrect about Phrap?

Which of the following is incorrect about ARACHNE?

VecScreen is a primarily aimed for sequence assembly.

When a sequence is generated from ____ ends of a single clone, the distance between the two opposing fragments of a clone is fixed to ________ meaning that they are always separated by a distance defined by a _____ length (normally 1,000 to 9,000 bases).

The major challenges in genome assembly are sequence errors, contamination by bacterial vectors, and repetitive sequence regions.

Which of the following is incorrect regarding Automated Genome Annotation?

Which of the following is incorrect about Phred?

In some circumstances, one mRNA transcript can lead to the translation of more than one protein.

Which of the following is incorrect regarding GeneQuiz?

Which of the following is incorrect regarding genome economy?

Conserved functional sites can be identified by profile and hidden Markov model–based motif and domain search tools such as SMART and InterPro.

The remote homology detection helps to shed light on the possible functions of the proteins that previously have no functional information at all.

Which of the following is incorrect regarding gene annotation?

The _ resolution genome map is the genomic DNA sequence that can be considered as a type of __ map describing a genome at the single base-pair level.

The shotgun approach ___ sequences clones from _ of cloned DNA.

Chloroplast localization signals are also located in the ____ terminus and are about 25 to 100 residues in length, containing very few _ charged residues but many hydroxylated residues such as serine.

The hierarchical approach is and _ than the shotgun approach because it involves an initial clone-based physical mapping step.

The signal sequences are typically _____ residues long, rich in _ charged residues such as arginines as well as hydroxyl residues such as serines and threonines, but devoid of ____ charged residues.

When a sequence is generated from ends of a single clone, the distance between the two opposing fragments of a clone is fixed to meaning that they are always separated by a distance defined by a _ length (normally 1,000 to 9,000 bases).