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This section includes 8 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
For carrying out gene manipulation, the use of cultured cells is _____ transgenic organisms. |
| A. | less reliable |
| B. | more reliable |
| C. | may be less or more reliable |
| D. | is same reliable as |
| Answer» B. more reliable | |
| 2. |
If controlled inactivation of a gene is carried out and some of the consequences when inactivation of a target gene is deleterious are avoided. It is referred as ____________ |
| A. | specialized gene targeting |
| B. | controlled gene targeting |
| C. | conditional gene targeting |
| D. | specific gene targeting |
| Answer» D. specific gene targeting | |
| 3. |
The gene targeting approach produces individuals which are ______ for inactivation of the gene. |
| A. | homozygous |
| B. | heterozygous |
| C. | either only homozygous or only heterozygous |
| D. | both heterozygous and homozygous |
| Answer» C. either only homozygous or only heterozygous | |
| 4. |
It is necessary to analyze the genome of transgenic cells to confirm that the site of integration is correct. This is carried out by southern blotting. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 5. |
If a gene is inactivated by gene targeting then it is called as ____________ |
| A. | knock-in gene |
| B. | knock-out gene |
| C. | gene disruption |
| D. | insertional inactivation |
| Answer» C. gene disruption | |
| 6. |
ES cells are used in order to ensure that insertion is done at the required chromosomal location and it is called as ____________ |
| A. | gene targeting |
| B. | knocking out |
| C. | knocking in |
| D. | gene disruption |
| Answer» B. knocking out | |
| 7. |
Embryonic stem cells (ES) are isolated and are injected again into the blastocoel of a developing embryo. The embryo which develops is entirely made up of these cells only. |
| A. | True |
| B. | False |
| Answer» C. | |
| 8. |
If the embryo is at one-cell stage then it is found in ____________ |
| A. | ovary |
| B. | oviduct |
| C. | uterus |
| D. | either ovary or uterus |
| Answer» C. uterus | |