Explore topic-wise MCQs in Biotechnology.

This section includes 33 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.

1.

Which of the following plant signal molecules regulate the expression of vir B, C, D and E in case of tobacco?
A. Acetosyringone
B. -hydroxy syringone
C. Both (a) and (b)
D. None of these
Answer» D. None of these
Discussion
2.

Liposomes mediated gene transfer in plants involves
A. plasmid DNA enclosed in a lipid bag
B. fusion of liposomes with protoplast
C. use of polyethylene glycol (PEG)
D. all of the above
Answer» E.
Discussion
3.

Which of the following are used as selection marker for the cells transformed with Agrobacterium?
A. Neomycin phosphotransferase
B. Streptomycin phosphotransferase
C. Hygromycin phosphotransferase
D. any of the above
Answer» E.
Discussion
4.

Vir genes required for the T-DNA transfer and processing are located
A. on the T-DNA
B. outside the T-DNA region
C. on the plant genome
D. none of these
Answer» C. on the plant genome
Discussion
5.

Microprojectile method of gene transfer in plants involves delivery of DNA
A. with the help of micromanipulator
B. with the help of bolistics
C. with the help of needles
D. any of the above
Answer» C. with the help of needles
Discussion
6.

Integrated octopine T DNA occurs as
A. single segment
B. two segments
C. three segments
D. four segments
Answer» C. three segments
Discussion
7.

The transfer of intermediate vectors into Agrobacterium are brought about by
A. transformation
B. biparental mating
C. triparental mating
D. transduction
Answer» D. transduction
Discussion
8.

Microinjection involves
A. injection of large amount of DNA
B. injection of DNA into bigger cells
C. injection with needle having diameter greater than cell diameter
D. all of the above
Answer» D. all of the above
Discussion
9.

The direct repeats flanking the T-DNA of Agrobacterium tume-faciens are known as
A. cos site
B. flanking sequences
C. border sequences
D. transfer sequences
Answer» D. transfer sequences
Discussion
10.

The left segment of octopine T-DNA (TL) is necessary for
A. enzymes for agropine biosynthesis
B. tumour formation
C. conjugative transfer
D. all of these
Answer» C. conjugative transfer
Discussion
11.

In the liposome mediated gene transfer in plants, nucleic acids are
A. protected from nuclease digestion
B. stable in liposomes
C. both (a) and (b)
D. not stable in liposomes
Answer» D. not stable in liposomes
Discussion
12.

Co-integrating transformation vectors must include a region of homology in
A. the vector plasmid
B. the Ti-plasmid
C. between vector plasmid and Ti-plasmid
D. none of these
Answer» D. none of these
Discussion
13.

Crown gall tissue
A. can be cultivated in vitro in absence of bacteria
B. retains tumorous properties when cultivated
C. both (a) and (b)
D. shows tumorous properties only in presence of bacteria
Answer» D. shows tumorous properties only in presence of bacteria
Discussion
14.

On Ti-plasmid T-region or T-DNA is flanked by a direct repeat of
A. 12 bp
B. 20 bp
C. 25 bp
D. 30 bp
Answer» D. 30 bp
Discussion
15.

Agrobacterium tumefaciens is a
A. gram (+) bacteria
B. gram (-) bacteria
C. a fungi
D. a yeast
Answer» C. a fungi
Discussion
16.

Opines are
A. amino acid derivatives found in tumor tissues
B. amino acid derivatives found in normal tissues
C. amino acid derivatives found in both normal as well as tumor tissues
D. none of the above
Answer» B. amino acid derivatives found in normal tissues
Discussion
17.

Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into
A. pBR 322 based plasmid vector
B. pRK 2013
C. pRN 3
D. all of these
Answer» B. pRK 2013
Discussion
18.

Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast?
A. It is carried out with the help of micromanipulator
B. The recipient cells are immobilized on artificial support or artificially bound to substarate
C. It employs needle with diameter greater than cell diameter
D. all of the above
Answer» D. all of the above
Discussion
19.

Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain
A. one set of sequence necessary for gene transfer
B. two sets of sequence necessary for gene transfer
C. three sets of sequence necessary for gene transfer
D. four sets of sequence necessary for gene transfer
Answer» C. three sets of sequence necessary for gene transfer
Discussion
20.

Opines that are present in crown gall tumour include
A. octopine
B. nopaline
C. agropine
D. all of these
Answer» E.
Discussion
21.

Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?
A. pRK 2013
B. pRN 3
C. either (a) or (b)
D. none of these
Answer» D. none of these
Discussion
22.

Which of the following is not true about the direct repeats flanking T-DNA?
A. They are conserved between nopaline and octopine Ti-plasmids
B. These repeats are transferred intact to the plant genome
C. These are important in integration mechanism
D. all of the above
Answer» C. These are important in integration mechanism
Discussion
23.

Virulence trait of Agrobacterium tumefaciens is borne on
A. chromosomal DNA
B. tumour inducing plasmid DNA
C. both chromosomal and plasmid DNA
D. cryptic plasmid DNA
Answer» C. both chromosomal and plasmid DNA
Discussion
24.

The size of the virulent plasmid of Agrobacterium tumefaciens is
A. 40-80 kb
B. 80-120 kb
C. 140-235 kb
D. >235 kb
Answer» D. >235 kb
Discussion
25.

Which of the following is true about T DNA?
A. Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA
B. Integration of T DNA occurs only at one specific sites in the plant nuclear DNA
C. Integration of T DNA occurs at two specific sites in the plant nuclear DNA
D. Integration of T DNA occurs at one site that may be random in the plant nuclear DNA
Answer» B. Integration of T DNA occurs only at one specific sites in the plant nuclear DNA
Discussion
26.

Which technique is used to introduce genes into dicots?
A. electroporation
B. particle acceleration
C. microinjection
D. Ti plasmid infection
Answer» E.
Discussion
27.

Integrated nopaline T-DNA occurs as
A. single segment
B. two segments
C. three segments
D. four segments
Answer» B. two segments
Discussion
28.

Advantage of microprojectile method over microinjection method for gene transfer in plants include
A. intact cells are used
B. method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall
C. gene can be transferred to many cells simultaneously
D. all of the above
Answer» E.
Discussion
29.

The right segment of octopine T-DNA (TR) is necessary for
A. enzymes for agropine biosynthesis
B. tumour formation
C. conjugative transfer
D. all of these
Answer» B. tumour formation
Discussion
30.

Direct DNA uptake by protoplasts can be stimulated by
A. polyethylene glycol (PEG)
B. decanal
C. luciferin
D. all of these
Answer» B. decanal
Discussion
31.

Plant transformation vectors based on Agrobacterium can generally be divided into
A. two vectors
B. four vectors
C. six vectors
D. eight vectors
Answer» B. four vectors
Discussion
32.

In a plant tumour cell
A. complete Ti plasmid is incorporated in plant nuclear DNA
B. different parts of the Ti-plasmid are incorporated
C. only a small specific segment of callus T DNA is incorporated
D. may vary from plant to plant
Answer» D. may vary from plant to plant
Discussion
33.

Which of the following is true about Agrobacterium tumefaciens?
A. It causes crown gall disease of plants
B. It infects gymosperms
C. It infects dicotyledonous angiosperms
D. All of the above
Answer» E.
Discussion