MCQOPTIONS
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This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
In fusion proteins, the reading frame in a vector is out of phase with the insert. |
| A. | True |
| B. | False |
| Answer» C. | |
| 2. |
Termination codon is required for stopping high levels of transcription of other regions such as _______ which might interfere with ___________ of the vector. |
| A. | origin, replication |
| B. | end, replication |
| C. | end, stability |
| D. | origin, stability |
| Answer» E. | |
| 3. |
Proteins synthesized by carrying out a translation of the vector region and continuing it in the insert region is called as _____________ |
| A. | hybrid protein |
| B. | fusion protein |
| C. | combination protein |
| D. | insert protein |
| Answer» C. combination protein | |
| 4. |
Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 5. |
If overexpression of proteins takes place, they become toxic at times. In such a case _______ colonies are recovered after ______ with the recombinant plasmid. |
| A. | few, translation |
| B. | few, transformation |
| C. | no, transformation |
| D. | no, translation |
| Answer» D. no, translation | |
| 6. |
TERMINATION_CODON_IS_REQUIRED_FOR_STOPPING_HIGH_LEVELS_OF_TRANSCRIPTION_OF_OTHER_REGION_SUCH_AS_________WHICH_MIGHT_INTERFERE_WITH______OF_THE_VECTOR.?$ |
| A. | origin, replication |
| B. | end, replication |
| C. | end, stability |
| D. | origin, stability |
| Answer» E. | |
| 7. |
In fusion proteins the reading frame in vector is out of phase with the insert. Is the given statement true or false?$ |
| A. | True |
| B. | False |
| Answer» C. | |
| 8. |
Which of the following is present in E. coli rrnB operon for rRNA? |
| A. | Initiation codon |
| B. | Termination codon |
| C. | Both initiation and termination codon |
| D. | Both are absent |
| Answer» D. Both are absent | |
| 9. |
It is also possible to generate a fusion protein in which ______ is encoded by the vector. |
| A. | N terminal |
| B. | C terminal |
| C. | both N and C terminal |
| D. | middle region |
| Answer» C. both N and C terminal | |
| 10. |
Proteins synthesized by carrying out translation of the vector region and continuing it in the insert region is called as: |
| A. | hybrid protein |
| B. | fusion protein |
| C. | combination protein |
| D. | insert protein |
| Answer» C. combination protein | |
| 11. |
If an expression vector contains all three, viz. a controllable promoter, a ribosome binding site and an initiation codon, then a hybrid protein is produced. The _______ region of hybrid protein is encoded by vector and the rest is expressed by the sequence inserted. |
| A. | N terminal |
| B. | C terminal |
| C. | both N & C terminal |
| D. | middle |
| Answer» B. C terminal | |
| 12. |
Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 13. |
Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site. |
| A. | ACG |
| B. | AUG |
| C. | UAC |
| D. | CAG |
| Answer» C. UAC | |
| 14. |
Ribosome binding site in E.coli is composed of which sequence primarily? |
| A. | -GAGG- |
| B. | -CTCC- |
| C. | -ATGG- |
| D. | -CGTT- |
| Answer» B. -CTCC- | |
| 15. |
If overexpression of proteins takes place, they become toxic at times. In such a case, _______ colonies are recovered after ______ with the recombinant plasmid. |
| A. | few, translation |
| B. | few, transformation |
| C. | no, transformation |
| D. | no, translation |
| Answer» D. no, translation | |