 
			 
			MCQOPTIONS
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				This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. | In fusion proteins, the reading frame in a vector is out of phase with the insert. | 
| A. | True | 
| B. | False | 
| Answer» C. | |
| 2. | Termination codon is required for stopping high levels of transcription of other regions such as _______ which might interfere with ___________ of the vector. | 
| A. | origin, replication | 
| B. | end, replication | 
| C. | end, stability | 
| D. | origin, stability | 
| Answer» E. | |
| 3. | Proteins synthesized by carrying out a translation of the vector region and continuing it in the insert region is called as _____________ | 
| A. | hybrid protein | 
| B. | fusion protein | 
| C. | combination protein | 
| D. | insert protein | 
| Answer» C. combination protein | |
| 4. | Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon. | 
| A. | True | 
| B. | False | 
| Answer» B. False | |
| 5. | If overexpression of proteins takes place, they become toxic at times. In such a case _______ colonies are recovered after ______ with the recombinant plasmid. | 
| A. | few, translation | 
| B. | few, transformation | 
| C. | no, transformation | 
| D. | no, translation | 
| Answer» D. no, translation | |
| 6. | TERMINATION_CODON_IS_REQUIRED_FOR_STOPPING_HIGH_LEVELS_OF_TRANSCRIPTION_OF_OTHER_REGION_SUCH_AS_________WHICH_MIGHT_INTERFERE_WITH______OF_THE_VECTOR.?$ | 
| A. | origin, replication | 
| B. | end, replication | 
| C. | end, stability | 
| D. | origin, stability | 
| Answer» E. | |
| 7. | In fusion proteins the reading frame in vector is out of phase with the insert. Is the given statement true or false?$ | 
| A. | True | 
| B. | False | 
| Answer» C. | |
| 8. | Which of the following is present in E. coli rrnB operon for rRNA? | 
| A. | Initiation codon | 
| B. | Termination codon | 
| C. | Both initiation and termination codon | 
| D. | Both are absent | 
| Answer» D. Both are absent | |
| 9. | It is also possible to generate a fusion protein in which ______ is encoded by the vector. | 
| A. | N terminal | 
| B. | C terminal | 
| C. | both N and C terminal | 
| D. | middle region | 
| Answer» C. both N and C terminal | |
| 10. | Proteins synthesized by carrying out translation of the vector region and continuing it in the insert region is called as: | 
| A. | hybrid protein | 
| B. | fusion protein | 
| C. | combination protein | 
| D. | insert protein | 
| Answer» C. combination protein | |
| 11. | If an expression vector contains all three, viz. a controllable promoter, a ribosome binding site and an initiation codon, then a hybrid protein is produced. The _______ region of hybrid protein is encoded by vector and the rest is expressed by the sequence inserted. | 
| A. | N terminal | 
| B. | C terminal | 
| C. | both N & C terminal | 
| D. | middle | 
| Answer» B. C terminal | |
| 12. | Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon. Is the given statement true or false? | 
| A. | True | 
| B. | False | 
| Answer» B. False | |
| 13. | Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site. | 
| A. | ACG | 
| B. | AUG | 
| C. | UAC | 
| D. | CAG | 
| Answer» C. UAC | |
| 14. | Ribosome binding site in E.coli is composed of which sequence primarily? | 
| A. | -GAGG- | 
| B. | -CTCC- | 
| C. | -ATGG- | 
| D. | -CGTT- | 
| Answer» B. -CTCC- | |
| 15. | If overexpression of proteins takes place, they become toxic at times. In such a case, _______ colonies are recovered after ______ with the recombinant plasmid. | 
| A. | few, translation | 
| B. | few, transformation | 
| C. | no, transformation | 
| D. | no, translation | 
| Answer» D. no, translation | |