Which of the following is untrue about DNA sequencing methods?

New strands of DNA are synthesized from the end of the primer

Purified fragments of DNA cut from plasmid/phage clones or amplified by polymerase chain reaction (PCR)

Clones of DNA fragments are denatured to single strands, and one of the strands is hybridized to an oligonucleotide primer

Taq polymerase is quite heat sensitive

New strands of DNA are synthesized from the end of the primer

Explore topic-wise MCQs in Bioinformatics.

This section includes 156 Mcqs, each offering curated multiple-choice questions to sharpen your Bioinformatics knowledge and support exam preparation. Choose a topic below to get started.

151.	To sequence larger molecules, individual chromosomes are purified and broken into _____ or larger random fragments, which are cloned into vectors designed for large molecules.
A.	100-Mb
B.	100-kb
C.	5000-kb
D.	600-kb
Answer» C. 5000-kb

Discussion

152.	When the process is fully automated, a number of priming sites may be used to obtain sequencing results that give optimal separation of bands in each region of the sequence.
A.	True
B.	False
Answer» B. False

Discussion

153.	The sequence can also be verified by making an oligonucleotide primer complementary to the distal part of the readable sequence and using it to obtain the sequence of the complementary strand on the original DNA template.
A.	True
B.	False
Answer» B. False

Discussion

154.	The combined mixture of all labeled DNA fragments is electrophoresed to _____ the fragments by______ and the ladder of fragments is scanned for the presence of each of the four labels.
A.	separate, size
B.	separate, pH
C.	assimilate, pH
D.	assimilate, size
Answer» B. separate, pH

Discussion

155.	Using ddATP, the resulting synthesis creates a set of nested DNA fragments, each one ending at one of the as in the sequence through the substitution of a fluorescent-labeled ddATP.
A.	True
B.	False
Answer» B. False

Discussion

156.	Which of the following is untrue about DNA sequencing methods?
A.	Purified fragments of DNA cut from plasmid/phage clones or amplified by polymerase chain reaction (PCR)
B.	Clones of DNA fragments are denatured to single strands, and one of the strands is hybridized to an oligonucleotide primer
C.	Taq polymerase is quite heat sensitive
D.	New strands of DNA are synthesized from the end of the primer
Answer» D. New strands of DNA are synthesized from the end of the primer

Discussion

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Explore topic-wise MCQs in Bioinformatics.

To sequence larger molecules, individual chromosomes are purified and broken into _____ or larger random fragments, which are cloned into vectors designed for large molecules.

When the process is fully automated, a number of priming sites may be used to obtain sequencing results that give optimal separation of bands in each region of the sequence.

The sequence can also be verified by making an oligonucleotide primer complementary to the distal part of the readable sequence and using it to obtain the sequence of the complementary strand on the original DNA template.

The combined mixture of all labeled DNA fragments is electrophoresed to _____ the fragments by______ and the ladder of fragments is scanned for the presence of each of the four labels.

Using ddATP, the resulting synthesis creates a set of nested DNA fragments, each one ending at one of the as in the sequence through the substitution of a fluorescent-labeled ddATP.

Which of the following is untrue about DNA sequencing methods?

The combined mixture of all labeled DNA fragments is electrophoresed to _ the fragments by__ and the ladder of fragments is scanned for the presence of each of the four labels.