MCQOPTIONS
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MCQOPTIONS
This section includes 10 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
NITROCELLULOSE_MEMBRANES_ARE_LESS_SENSITIVE_THAN_NYLON_MEMBRANES._IS_THE_GIVEN_STATEMENT_TRUE_OR_FALSE??$ |
| A. | True |
| B. | False |
| Answer» C. | |
| 2. |
What_is_used_for_lysing_of_bacterial_cells_and_denaturation_of_DNA?$ |
| A. | Exonuclease |
| B. | Sulphuric Acid |
| C. | Sodium Hydroxide |
| D. | Heat |
| Answer» D. Heat | |
| 3. |
Choose the incorrect statement with respect to the membranes used for adhering of bacterial cells onto them? |
| A. | Nitrocellulose membranes were preferred earlier |
| B. | They bind DNA very efficiently |
| C. | They can be handled easily without breakage |
| D. | They are inflammable |
| Answer» D. They are inflammable | |
| 4. |
The screening of libraries us carried out by nucleic acid hybridisation and constitutes of following steps: |
| A. | Peeling of membranes carrying away bacterial cells with it |
| B. | Cells are lysed and denaturing of DNA is being carried out |
| C. | Hybridization with labelled DNA |
| D. | Placing the membrane onto plate containing recombinant cells |
| Answer» B. Cells are lysed and denaturing of DNA is being carried out | |
| 5. |
Choose the incorrect statement for colony or plaque lift. |
| A. | It is the base for screening based on nucleic acid hybridization |
| B. | It is also known as Grunstein-Hogness technique |
| C. | It is based on the fact that which members of the library have the same sequence as the DNA probe |
| D. | The library may be plaques on a bacterial lawn and in that case it is known as plaque lift |
| Answer» D. The library may be plaques on a bacterial lawn and in that case it is known as plaque lift | |
| 6. |
How many techniques are there for carrying out the screening of sequences encoding for RNAs? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 7. |
If screening is carried out on the basis of sequences which are related to the desired sequence, then the process is called as: |
| A. | in-silico |
| B. | homologue search |
| C. | annotation |
| D. | partial search |
| Answer» D. partial search | |
| 8. |
If we are having sequence data for a particular organism, but screening is carried out for homologues the program used is BLAST. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 9. |
If for a particular organism sequence data is available and we have to simply search in the data through computer, then this method is called as: |
| A. | annotation |
| B. | database search |
| C. | in silico |
| D. | electronic search |
| Answer» D. electronic search | |
| 10. |
The process of finding a particular member of library which is having some defined properties is called as: |
| A. | searching |
| B. | screening |
| C. | locating |
| D. | narrowing |
| Answer» C. locating | |