Glucorticoid-responsive element is ______ promoter from ______ of mouse mammory tumour virus (MMTV).

constitutive, short terminal repeat region

inducible, short terminal repeat region

inducible, long terminal repeat region

constitutive, short terminal repeat region

constitutive, long terminal repeat region

Choose the incorrect statement in respect to MACs.

It can be maintained for long term

They are mammalian or human artificial chromosomes

Large stretches of DNA (in excess of 100 kb) can be inserted

A selectable marker is required

It can be maintained for long term

The retroviral transfected DNA is infected into mammalian cells by helper retrovirus. Helper retrovirus should not be packaged and the method adopted for this is?

Deletion of the packaging signal in the helper genome

Deletion of multiple parts of the helper genome

Division of the helper genome into several pieces

LNSX is a retrovirus and is combined with ______ in order to create a shuttle vector.

either eukaryotic or prokaryotic plasmid

T protein is required for replication. It is a product of ____________

both early and late replication

15 + Mcqs in Cultured Cells in Mammals and Restricted Areas in Intact Organisms 2 Page 1 McqOptions

1.	The amount of expression of DNA inserted into a vector _______ on promoter and ______ on chromosomal location.
A.	depends only on, not depends
B.	depends, depends also
C.	doesn t depends, but depends
D.	neither depends, nor depends
Answer» C. doesn t depends, but depends

Discussion

2.	There are a group of promoters from the genes for metallothioneins. These are a group of proteins rich in ______ residues.
A.	cysteine
B.	methionine
C.	either cysteine or methionine
D.	cysteine and methionine both
Answer» B. methionine

Discussion

3.	Glucorticoid-responsive element is ______ promoter from ______ of mouse mammory tumour virus (MMTV).
A.	inducible, short terminal repeat region
B.	inducible, long terminal repeat region
C.	constitutive, short terminal repeat region
D.	constitutive, long terminal repeat region
Answer» C. constitutive, short terminal repeat region

Discussion

4.	Expression of tTA gene is ________ by doxycycline which is _______ analogue.
A.	activated, tetracycline
B.	inactivated, chloramphenicol
C.	activated, chloramphenicol
D.	inactivated, tetracycline
Answer» E.

Discussion

5.	Tetracycline binding repressor protein when fused to transcription activator protein from herpes simple virus, then it is called as transactivator protein (tTA).
A.	True
B.	False
Answer» B. False

Discussion

6.	In naturally occurring bacterial systems, tetracycline binding repressor protein binds in the _____ of tetracycline to the control sequence and _______ transcription.
A.	presence, activates
B.	absence, activates
C.	absence, inactivates
D.	presence, inactivates
Answer» D. presence, inactivates

Discussion

7.	Constitutive promoters are majorly obtained from ________
A.	fungi
B.	bacteria
C.	mammalian cells
D.	viral cells
Answer» E.

Discussion

8.	Centromere is the most important component of the human artificial chromosome. It is constructed from tandemly repeated copies of 171 bp of ______ DNA sequence.
A.	alpha satellite
B.	beta satellite
C.	satellite 2
D.	satellite 1
Answer» B. beta satellite

Discussion

9.	Choose the incorrect statement in respect to MACs.
A.	They are mammalian or human artificial chromosomes
B.	Large stretches of DNA (in excess of 100 kb) can be inserted
C.	A selectable marker is required
D.	It can be maintained for long term
Answer» D. It can be maintained for long term

Discussion

10.	The retroviral transfected DNA is infected into mammalian cells by helper retrovirus. Helper retrovirus should not be packaged and the method adopted for this is?
A.	Deletion of the packaging signal in the helper genome
B.	Deletion of multiple parts of the helper genome
C.	Division of the helper genome into several pieces
D.	All of the mentioned
Answer» E.

Discussion

11.	LNSX is a retrovirus and is combined with ______ in order to create a shuttle vector.
A.	eukaryotic plasmid
B.	prokaryotic plasmid
C.	either eukaryotic or prokaryotic plasmid
D.	yeast plasmid
Answer» C. either eukaryotic or prokaryotic plasmid

Discussion

12.	It is beneficial to use retrovirus for genetic manipulation of cells in the culture.
A.	True
B.	False
Answer» B. False

Discussion

13.	Vaccinia virus produces its own ______ which is functional in _____ of infected cells where the virus also replicates.
A.	DNA polymerase, nucleus
B.	DNA polymerase, cytoplasm
C.	RNA polymerase, cytoplasm
D.	RNA polymerase, nucleus
Answer» D. RNA polymerase, nucleus

Discussion

14.	Vaccinia virus is a commonly used virus and it is a member of pox virus family. It has a large genome of _____________ and can accept at least ____________ of foreign DNA.
A.	200kb, 25kb
B.	1000kb, 200kb
C.	500kb, 50kb
D.	800kb, 100kb
Answer» B. 1000kb, 200kb

Discussion

15.	T protein is required for replication. It is a product of ____________
A.	delayed early transcription
B.	late transcription
C.	early transcription
D.	both early and late replication
Answer» D. both early and late replication

Discussion

Explore topic-wise MCQs in Genetic Engineering.

The amount of expression of DNA inserted into a vector _ on promoter and on chromosomal location.

There are a group of promoters from the genes for metallothioneins. These are a group of proteins rich in ______ residues.

Glucorticoid-responsive element is promoter from of mouse mammory tumour virus (MMTV).

Expression of tTA gene is __ by doxycycline which is _ analogue.

Tetracycline binding repressor protein when fused to transcription activator protein from herpes simple virus, then it is called as transactivator protein (tTA).

In naturally occurring bacterial systems, tetracycline binding repressor protein binds in the _ of tetracycline to the control sequence and ___ transcription.

Constitutive promoters are majorly obtained from ________

Centromere is the most important component of the human artificial chromosome. It is constructed from tandemly repeated copies of 171 bp of ______ DNA sequence.

Choose the incorrect statement in respect to MACs.

The retroviral transfected DNA is infected into mammalian cells by helper retrovirus. Helper retrovirus should not be packaged and the method adopted for this is?

LNSX is a retrovirus and is combined with ______ in order to create a shuttle vector.

It is beneficial to use retrovirus for genetic manipulation of cells in the culture.

Vaccinia virus produces its own __ which is functional in _ of infected cells where the virus also replicates.

Vaccinia virus is a commonly used virus and it is a member of pox virus family. It has a large genome of _ and can accept at least of foreign DNA.

T protein is required for replication. It is a product of ____________

Explore topic-wise MCQs in Genetic Engineering.

The amount of expression of DNA inserted into a vector _______ on promoter and ______ on chromosomal location.

There are a group of promoters from the genes for metallothioneins. These are a group of proteins rich in ______ residues.

Glucorticoid-responsive element is ______ promoter from ______ of mouse mammory tumour virus (MMTV).

Expression of tTA gene is ________ by doxycycline which is _______ analogue.

Tetracycline binding repressor protein when fused to transcription activator protein from herpes simple virus, then it is called as transactivator protein (tTA).

In naturally occurring bacterial systems, tetracycline binding repressor protein binds in the _____ of tetracycline to the control sequence and _______ transcription.

Constitutive promoters are majorly obtained from ________

Centromere is the most important component of the human artificial chromosome. It is constructed from tandemly repeated copies of 171 bp of ______ DNA sequence.

Choose the incorrect statement in respect to MACs.

The retroviral transfected DNA is infected into mammalian cells by helper retrovirus. Helper retrovirus should not be packaged and the method adopted for this is?

LNSX is a retrovirus and is combined with ______ in order to create a shuttle vector.

It is beneficial to use retrovirus for genetic manipulation of cells in the culture.

Vaccinia virus produces its own ______ which is functional in _____ of infected cells where the virus also replicates.

Vaccinia virus is a commonly used virus and it is a member of pox virus family. It has a large genome of _____________ and can accept at least ____________ of foreign DNA.

T protein is required for replication. It is a product of ____________

The amount of expression of DNA inserted into a vector _ on promoter and on chromosomal location.

Glucorticoid-responsive element is promoter from of mouse mammory tumour virus (MMTV).

Expression of tTA gene is __ by doxycycline which is _ analogue.

In naturally occurring bacterial systems, tetracycline binding repressor protein binds in the _ of tetracycline to the control sequence and ___ transcription.

Vaccinia virus produces its own __ which is functional in _ of infected cells where the virus also replicates.

Vaccinia virus is a commonly used virus and it is a member of pox virus family. It has a large genome of _ and can accept at least of foreign DNA.