Explore topic-wise MCQs in Bioinformatics.

This section includes 11 Mcqs, each offering curated multiple-choice questions to sharpen your Bioinformatics knowledge and support exam preparation. Choose a topic below to get started.

1.

The PCR amplification step involved in the SAGE procedure means that it requires a large quantity of sample mRNA.

A. True
B. False
Answer» B. False
Discussion
2.

DNA microarrays measure “absolute” mRNA expression levels without arbitrary reference standards, whereas SAGE indicates the relative expression levels.

A. True
B. False
Answer» C.
Discussion
3.

Which of the following is untrue about SAGE Genie?

A. It is an NCBI web-based program
B. It allows matching of experimentally obtained SAGE tags to known genes
C. It provides an interface for visualizing human gene expression
D. It doesn’t filter out linker sequences from experimentally obtained SAGE tags
Answer» E.
Discussion
4.

SAGExProfiler doesn’t provide information about overexpressed or silenced genes.

A. True
B. False
Answer» C.
Discussion
5.

THE_PCR_AMPLIFICATION_STEP_INVOLVED_IN_THE_SAGE_PROCEDURE_MEANS_THAT_IT_REQUIRES_A_LARGE_QUANTITY_OF_SAMPLE_MRNA.?$

A. True
B. False
Answer» B. False
Discussion
6.

Which_of_the_following_is_an_incorrect_statement?$

A. Collecting a SAGE library is very labor intensive and expensive
B. Collecting a SAGE library is quite economical
C. SAGE is not suitable for rapid screening of cells
D. Gene identification from SAGE data is also more cumbersome
Answer» C. SAGE is not suitable for rapid screening of cells
Discussion
7.

DNA microarrays measure “absolute” mRNA expression levels without arbitrary reference standards, whereas SAGE indicates the relative expression levels?#

A. True
B. False
Answer» C.
Discussion
8.

SAGExProfiler doesn’t provide information about overexpressed or silenced genes$

A. True
B. False
Answer» C.
Discussion
9.

SAGEmap is a SAGE database created by NCBI.

A. True
B. False
Answer» B. False
Discussion
10.

Which of the following is untrue about the drawbacks of SAGE?

A. One or two sequencing errors in the tag sequence can lead to ambiguous or erroneous tag identification
B. Correctly sequenced SAGE tag sometimes may correspond to several genes or no gene at all
C. Correctly sequenced SAGE tag always corresponds to several genes
D. The drawback with this approach is the sensitivity to sequencing errors
Answer» D. The drawback with this approach is the sensitivity to sequencing errors
Discussion
11.

Which of the following is untrue about SAGE?

A. This approach is much more efficient than the EST analysis
B. This approach is quite less efficient than the EST analysis
C. It uses a short nucleotide tag to define a gene transcript
D. It allows sequencing of multiple tags in a single clone
Answer» C. It uses a short nucleotide tag to define a gene transcript
Discussion