MCQOPTIONS
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MCQOPTIONS
This section includes 7 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The polypeptides produced after addition of mRNA are analysed with antibodies. Choose the incorrect statement for this analysis. |
| A. | Antibodies are added to each reaction tube and precipitation is simply based on antigen-antibody reaction |
| B. | Along with simple antigen-antibody complex, a substrate is added for easy precipitation |
| C. | Protein A-Sepharose is added |
| D. | Protein A-Sepharose binds to IgG antibodies |
| Answer» B. Along with simple antigen-antibody complex, a substrate is added for easy precipitation | |
| 2. |
What is done after RNA fractionation is carried out? |
| A. | Each band is translated in vivo |
| B. | Translation is carried out in wheat gram or lysate of rabbit reticulocyte cells |
| C. | Translation is carried out with a high background |
| D. | Amino acid is not radioactively labelled |
| Answer» C. Translation is carried out with a high background | |
| 3. |
Choose the correct statement for RNA fractionation. |
| A. | The RNA is fractioned by size but before separating on oligo-dT cellulose |
| B. | A sucrose density gradient is used |
| C. | The RNA is applied to the top of a pre-poured gradient and during centrifugation smaller molecules move down the tube faster |
| D. | Different bands are formed according to the density in the sucrose density gradient |
| Answer» C. The RNA is applied to the top of a pre-poured gradient and during centrifugation smaller molecules move down the tube faster | |
| 4. |
What do we mean by housekeeping genes? |
| A. | Housekeeping genes are those genes which are specific to an organism |
| B. | Housekeeping genes are those genes which are present in all the organisms |
| C. | Housekeeping genes are those genes which are meant for repair and maintenance in a species of organism |
| D. | Housekeeping genes are those genes which required for the replication process |
| Answer» C. Housekeeping genes are those genes which are meant for repair and maintenance in a species of organism | |
| 5. |
Any cDNA library would represent a fraction of RNA species of an organism. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 6. |
In case if molecules smaller than the fragments required for making a full genomic library are used for making a collection. This collection is called as ___________ |
| A. | library |
| B. | shelf |
| C. | small library |
| D. | mini library |
| Answer» C. small library | |
| 7. |
By synthesizing two strands separately then annealing them leads to formation of double stranded oligonucleotide. |
| A. | True |
| B. | False |
| Answer» B. False | |