MCQOPTIONS
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MCQOPTIONS
This section includes 13 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Chain-termination mutations in coat proteins genes are carried out. The coat protein coat genes used are __________ |
| A. | A gene |
| B. | D gene |
| C. | E and D gene |
| D. | D and A gene |
| Answer» D. D and A gene | |
| 2. |
Packaging in vitro is basically carried out by two different strategies. Choose the incorrect statement for it. |
| A. | Separate strains with chain-termination mutations in different genes for coat components are used __________ |
| B. | Temperature sensitive cI repressor is used |
| C. | Due to heat shock packaging proteins are induced |
| D. | Packaging is carried out in each strain |
| Answer» E. | |
| 3. |
Packaging in vitro is also carried out to introduce the lambda DNA into the cells. Choose the incorrect statement in respect to it. |
| A. | Packaging in phage coat is a more efficient process than the normal transformation process |
| B. | The lambda DNA is incubated with lysate of lambda infected cells in a concatemeric configuration |
| C. | Lysate constitutes of lambda proteins needed for phage assembly |
| D. | The amount of non-recombinant phage in the background is less |
| Answer» E. | |
| 4. |
Physical separation is also used at times. Choose the correct statement with respect to this method. |
| A. | Physical separation constitutes of gel electrophoresis and or by using centrifugation in sugar density gradient |
| B. | In sugar density gradient, the molecules are separated on the basis of density |
| C. | The central portion is recovered from the gel or the gradient |
| D. | Gel is more efficient than the sugar density gradient |
| Answer» B. In sugar density gradient, the molecules are separated on the basis of density | |
| 5. |
At times, a second enzyme is used while the central portion is removed and the stuffer fragment is placed there. Choose the statement which doesn’t holds for this process. |
| A. | It is a method which is used for avoiding the relegation of central portion before the stuffer fragment is attached over there |
| B. | In this method, the central portion is cut with another enzyme after being separated from the arms |
| C. | The arms are also cut with this second enzyme |
| D. | It is not necessary to remove the fragments generated |
| Answer» D. It is not necessary to remove the fragments generated | |
| 6. |
Is it necessary to stop the relegation of the central portion before the stiffer fragment is ligated. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 7. |
Choose incorrect statement for the substitution vector EMBL4. |
| A. | It is having two single multiple cloning sites |
| B. | The region to be removed is on one side of the multiple cloning sites |
| C. | If a digestion within the multiple cloning sites, it generates central portion and right and left arms |
| D. | The right and left portions are called as arms |
| Answer» C. If a digestion within the multiple cloning sites, it generates central portion and right and left arms | |
| 8. |
The fragment inserted in the place of the central portion of the genome is known as __________ |
| A. | insertion fragment |
| B. | substitution fragment |
| C. | stuffer fragment |
| D. | displacement fragment |
| Answer» D. displacement fragment | |
| 9. |
Choose the incorrect statement for the central portion of the lambda genome. |
| A. | The central portion is not required for lytic growth |
| B. | The central portion includes repressor gene |
| C. | The repressor gene is not required for lysogenic growth |
| D. | It is approximately 10kbp long |
| Answer» E. | |
| 10. |
A portion of phage is removed and in place of it, the DNA of interest is inserted. This type of vector is called as __________ |
| A. | displacement vector |
| B. | insertion vector |
| C. | substitution vector |
| D. | transposition vector |
| Answer» D. transposition vector | |
| 11. |
The presence of insert leads to inactivation of which gene? |
| A. | cII |
| B. | cI |
| C. | cIII |
| D. | both cII and cIII |
| Answer» C. cIII | |
| 12. |
Choose the correct statement for lambda gt10. |
| A. | It is having an EcoRI site |
| B. | It is having cII gene |
| C. | It accepts an insert of size greater than that of 8 kbp |
| D. | This size is less than that the size which is generally accepted by wild type phage |
| Answer» B. It is having cII gene | |
| 13. |
Insertion vectors are a type of vectors. Choose the correct statement for this type of vector. |
| A. | These are complex vectors |
| B. | DNA is inserted through many restriction sites |
| C. | There is an upper limit on the size of the DNA to be packaged |
| D. | Lambda gt09 is an example of this type of vector |
| Answer» D. Lambda gt09 is an example of this type of vector | |