Explore topic-wise MCQs in Genetic Engineering.

This section includes 13 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

There are some phages which don t preferentially transduce some special regions of the phage genome. These phages are known as __________

A. transducing phages
B. specialized transducing phages
C. generalized transducing phages
D. transforming phages
Answer» D. transforming phages
Discussion
2.

There is a limit on upper size of the DNA to be packed. Choose the correct statement with respect to phages in this context.

A. There is some phage DNA lost in this process
B. The phages are known as transformed phages
C. These type of phages can t be selected and harvested
D. Lambda is not a special attachment site
Answer» B. The phages are known as transformed phages
Discussion
3.

The replication rate remains the same for all the phages irrespective of what sequence is there in the phage.

A. True
B. False
Answer» C.
Discussion
4.

The essential sites for recombination are known as __________

A. chi sites
B. rec sites
C. gam sites
D. red sites
Answer» B. rec sites
Discussion
5.

Choose the correct statement for RecBCD nuclease.

A. It promotes rolling circle replication
B. It is blocked by Gam protein
C. It blocks theta mode of replication
D. It is blocked by Red protein
Answer» C. It blocks theta mode of replication
Discussion
6.

Phages which are designated as spi-are __________

A. red+ gam+
B. red+ gam-
C. red- gam+
D. red- gam-
Answer» E.
Discussion
7.

The red and gam genes are removed in which type of phages?

A. Substitution phage
B. Replacement and substitution phages both
C. Replacement phage
D. Substitution is preferred over replacement phage
Answer» D. Substitution is preferred over replacement phage
Discussion
8.

What is the function of red and gam gene products?

A. It promotes the growth of the phage in the E. coli cells which are lysogenic for bacteriophage P1
B. It inhibits the growth of the phage in the E. coli cells which are lysogenic for bacteriophage P2
C. It inhibits the growth of the phage in the E. coli cells which are lytic for bacteriophage P1
D. It activates the growth of the phage in the E. coli cells which are lytic for bacteriophage P2
Answer» C. It inhibits the growth of the phage in the E. coli cells which are lytic for bacteriophage P1
Discussion
9.

The cI function can be scanned in order to check whether the recombinants are present or not. Which of the following doesn t hold true?

A. The cI protein is required for the formation of lysogen
B. The plaques formed are turbid in the case if the cI gene is active
C. The plaques formed are clear in the case if the cI gene is inactive
D. hflA mutant host reduces the amount of cII stability
Answer» E.
Discussion
10.

If a single system is used for packaging, there are increased chances of endogenous material being packed.

A. True
B. False
Answer» B. False
Discussion
11.

Which of the following is not a consequence of deletion in the b region?

A. Excision of the prophage on induction is prevented
B. It reduces the amount of endogenous phage DNA in the packaging mix
C. It reduces the amount of exogenous phage DNA in the packaging mix
D. It reduces the level of background non-recombinant phage
Answer» D. It reduces the level of background non-recombinant phage
Discussion
12.

Which of the following is used for blocking the phage-encoded recombination?

A. Mutation in D gene
B. Mutation in E gene
C. Red mutation
D. Mutation in S gene
Answer» D. Mutation in S gene
Discussion
13.

What happens if chain-termination mutation is in the S gene?

A. Cell lysis is blocked
B. Growth of cells containing low levels of packaging proteins is not allowed
C. The lysis of cells is not carried artificially
D. Packaging is not carried out efficiently
Answer» B. Growth of cells containing low levels of packaging proteins is not allowed
Discussion