MCQOPTIONS
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MCQOPTIONS
This section includes 13 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
WHAT_DO_WE_MEAN_BY_HOUSEKEEPING_GENES??$ |
| A. | Housekeeping genes are those genes which are specific to an organism |
| B. | Housekeeping genes are those genes which are present in all the organisms |
| C. | Housekeeping genes are those genes which are meant for repair and maintenance in a species of organism |
| D. | Housekeeping genes are those genes which required for replication process |
| Answer» C. Housekeeping genes are those genes which are meant for repair and maintenance in a species of organism | |
| 2. |
What is done after RNA fractionation is carried out?$ |
| A. | Each band is translated in vivo |
| B. | Translation is carried out in wheat gram or lysate of rabbit reticulocyte cells |
| C. | Translation is carried out with a high background |
| D. | Amino acid is not radioactively labelled |
| Answer» C. Translation is carried out with a high background | |
| 3. |
Choose_the_correct_statement_for_RNA_fractionation.$ |
| A. | The RNA is fractioned by size but before separating on oligo-dT cellulose |
| B. | A sucrose density gradient is used |
| C. | The RNA is applied to the top of a pre-poured gradient and during centrifugation smaller molecules move down the tube faster |
| D. | Different bands are formed according to the density in the sucrose density gradient |
| Answer» C. The RNA is applied to the top of a pre-poured gradient and during centrifugation smaller molecules move down the tube faster | |
| 4. |
What is done after the recovery of pellets has been carried out in order to know the amount of polypeptides? |
| A. | Denaturing and gel electrophoresis in SDS- Polyacryamide gel |
| B. | Gel electrophoresis in agarose gel |
| C. | Quantitative PCR |
| D. | Weighing pellets |
| Answer» B. Gel electrophoresis in agarose gel | |
| 5. |
The_polypeptides_produced_after_addition_of_mRNA_are_analysed_with_antibodies._Choose_the_incorrect_statement_for_this_analysis. |
| A. | Antibodies are added to each reaction tube and precipitation is simply based on antigen-antibody reaction |
| B. | Along with simple antigen-antibody complex, a substrate is added for easy precipitation |
| C. | Protein A-Sepharose is added |
| D. | Protein A-Sepharose binds to IgG antibodies |
| Answer» B. Along with simple antigen-antibody complex, a substrate is added for easy precipitation | |
| 6. |
Any cDNA library would represent a fraction of RNA species of an organism. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 7. |
Choose the correct statement for construction of a library subsection. |
| A. | The size of a particular restriction fragment on which the gene is located is not known |
| B. | The size of the restriction fragment can be known by carrying out southern blotting |
| C. | Another digest of the genomic DNA is carried out by different enzyme |
| D. | DNA fragments of different size are recovered after carrying out gel electrophoresis |
| Answer» C. Another digest of the genomic DNA is carried out by different enzyme | |
| 8. |
In case if molecules smaller than the fragments required for making a full genomic library, are used for making a collection. This collection is called as: |
| A. | library |
| B. | shelf |
| C. | small library |
| D. | mini library |
| Answer» C. small library | |
| 9. |
Choose the correct statement if the RNA is non polydenylated. |
| A. | A collection of chemically synthesized oligonucleotides is used as primers |
| B. | They are usually tetramer |
| C. | Unequal quantities of A, G, T and C are used |
| D. | The primers attach at only specific sequences for first strand synthesis |
| Answer» B. They are usually tetramer | |
| 10. |
Choose the incorrect statement for the homopolymer tailing of cDNA strands. |
| A. | The blunt ended double stranded cDNA molecules are treated with terminal transferase and dCTPs |
| B. | Vector is also treated with terminal transferase and dGTPs |
| C. | The vector and cDNA can now anneal with the help of DNA ligase |
| D. | If gaps are created they can be repaired by physiological processes |
| Answer» D. If gaps are created they can be repaired by physiological processes | |
| 11. |
By synthesizing two strands separately then annealing them leads to formation of double stranded oligonucleotide. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 12. |
Choose the correct statement for modification of homopolymer tailing method. |
| A. | It includes modification of primers |
| B. | Primers are varied by simply altering their size by randomly adding or removing bases |
| C. | The 5’ end of the first cDNA strand is tailed with C residues |
| D. | A single stranded oilgonucleotide is then used for second strand synthesis |
| Answer» B. Primers are varied by simply altering their size by randomly adding or removing bases | |
| 13. |
RNaseH method and homopolymer tailing method generates blunt ended cDNA molecules. Which of the following can be used for attaching them to vector? |
| A. | Blunt ended ligation |
| B. | Addition of linkers |
| C. | Using appropriate restriction enzymes |
| D. | All the methods can be used equivalently |
| Answer» E. | |