MCQOPTIONS
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MCQOPTIONS
This section includes 10 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
What is the second primer in the case of 5 RACE? |
| A. | Internal primer |
| B. | Oligo-dA sequence |
| C. | Adaptor-oligo-dT primer |
| D. | Oligo-dT adaptor molecule |
| Answer» D. Oligo-dT adaptor molecule | |
| 2. |
The first cDNA strand in 5 RACE is tailed with oligo-dA tail. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 3. |
The first primer in the case of 3 RACE is ___________ |
| A. | internal sequence |
| B. | oligo-dT adaptor molecule |
| C. | oligo-dA adaptor molecule |
| D. | adaptor oligo-dT primer |
| Answer» C. oligo-dA adaptor molecule | |
| 4. |
Choose the correct statement for RACE. |
| A. | It stands for Random Amplification of cDNA ends |
| B. | It is for cloning particular cDNA ends |
| C. | It is only of one type, which is 5 RACE |
| D. | Sequence data is not available in any case |
| Answer» C. It is only of one type, which is 5 RACE | |
| 5. |
Choose the incorrect statement for the method homopolymer tailing. |
| A. | The first step is the RNA: DNA hybrid synthesis |
| B. | Terminal transferase is used for the addition of nucleotides on 3 end |
| C. | Terminal transferase adds only at DNA strands |
| D. | The DNA strand is now having known sequence at 3 end |
| Answer» D. The DNA strand is now having known sequence at 3 end | |
| 6. |
Choose the correct statement with respect to the self priming method of cDNA synthesis. |
| A. | It is less preferred than RNaseH method |
| B. | A hairpin structure is formed with guarantee |
| C. | The sequence corresponding to the 5 end is lost |
| D. | Reverse transcriptase is not used |
| Answer» D. Reverse transcriptase is not used | |
| 7. |
The loop region is single stranded. It can be cleaved by using which enzyme? |
| A. | Exonuclease |
| B. | S1 nuclease |
| C. | RNaseH |
| D. | DNase |
| Answer» C. RNaseH | |
| 8. |
What would not happen if the RNA strand is completely removed from RNA: DNA hybrid? |
| A. | There are no chances of the synthesis of the second DNA strand |
| B. | Chance complementarity would take place |
| C. | Hairpin structure would be formed |
| D. | Hairpin structure is formed is not the final structure |
| Answer» B. Chance complementarity would take place | |
| 9. |
What is the final product of the RNaseH method? |
| A. | blunt ended dsDNA |
| B. | staggered dsDNA at both ends |
| C. | staggered dsDNA at 3 end |
| D. | staggered dsDNA at 5 end |
| Answer» B. staggered dsDNA at both ends | |
| 10. |
Choose the incorrect statement for second DNA strand synthesis. |
| A. | The RNA: DNA complex is treated with the enzyme RNaseH and DNA pol |
| B. | The enzyme RNaseH is responsible for nicking the RNA strand and leaving free 3 hydroxyl ends |
| C. | These RNA fragments can be used as primers |
| D. | There is no portion of RNA left attached to the DNA strand |
| Answer» E. | |