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				This section includes 48 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.
| 1. | The organism used for the preparation of sake is | 
| A. | <i>Rhizopus sonti</i> | 
| B. | <i>Aspergillus oryzae</i> | 
| C. | <i>Lactobacillus vermiformis</i> | 
| D. | <i>Saccharomyces pyriformis</i> | 
| Answer» C. <i>Lactobacillus vermiformis</i> | |
| 2. | The organism used for the preparation of sonti is | 
| A. | <i>Rhizopus sonti</i> | 
| B. | <i>Aspergillus oryzae</i> | 
| C. | <i>Lactobacillus vermiformis</i> | 
| D. | <i>Saccharomyces pyriformis</i> | 
| Answer» B. <i>Aspergillus oryzae</i> | |
| 3. | Sonti is | 
| A. | rice beer or wine of India | 
| B. | wheat beer or wine | 
| C. | barley beer or wine | 
| D. | rice beer or wine of Japan | 
| Answer» B. wheat beer or wine | |
| 4. | What temperature is maintained during anaerobic fermentation of white wine? | 
| A. | 10-21 C | 
| B. | 21-30 C | 
| C. | 30-35 C | 
| D. | 15-25 C | 
| Answer» B. 21-30 C | |
| 5. | Maturation of the beers is carried out at | 
| A. | 14 C | 
| B. | 10 C | 
| C. | 6 C | 
| D. | 2 C | 
| Answer» E. | |
| 6. | Wine is obtained after alcoholic fermentation of | 
| A. | rice | 
| B. | wheat | 
| C. | grapes | 
| D. | barley | 
| Answer» D. barley | |
| 7. | After storage and recarbonation, left over yeast is separated by | 
| A. | centrifugation | 
| B. | filtration | 
| C. | cell disruption | 
| D. | need not to be separated | 
| Answer» C. cell disruption | |
| 8. | Which of the following is true for the lager beer? | 
| A. | Bottom fermenting yeast is used for the preparation | 
| B. | Top yeast is used for the preparation | 
| C. | Require high fermentation temperature | 
| D. | Both (b) and (c) | 
| Answer» B. Top yeast is used for the preparation | |
| 9. | Fining a wine is defined as | 
| A. | adding one substance to remove another | 
| B. | removing small particles | 
| C. | adding acid | 
| D. | removing tannin | 
| Answer» B. removing small particles | |
| 10. | Malolactic fermentation | 
| A. | produces lactic acid | 
| B. | reduces total acidity | 
| C. | produces diacetyl | 
| D. | all of these | 
| Answer» E. | |
| 11. | A hydrometer | 
| A. | measures wine acid | 
| B. | requires a few drops of juice | 
| C. | measures alcohol in the wine | 
| D. | measures grape sugar | 
| Answer» E. | |
| 12. | Punching down is done | 
| A. | for the same reasons as pumping over | 
| B. | to extract tannin | 
| C. | both (a) and (b) | 
| D. | to keep the cap dry | 
| Answer» E. | |
| 13. | Sodium or potassium meta bi sulphate is added to crushed grapes to | 
| A. | enhance the flavouring compound | 
| B. | check the undesirable organisms | 
| C. | maintain pH | 
| D. | all of the above | 
| Answer» C. maintain pH | |
| 14. | The process of making malt as soluble as possible by using enzymes adjuncts etc is known as | 
| A. | brewing | 
| B. | malting | 
| C. | mashing | 
| D. | pitching | 
| Answer» D. pitching | |
| 15. | What temperature is maintained during anaerobic fermentation of red wine? | 
| A. | 20-24 C | 
| B. | 24-27 C | 
| C. | 27-31 C | 
| D. | 31-34 C | 
| Answer» C. 27-31 C | |
| 16. | If the malolactic fermentation is not carried out, | 
| A. | the wine will have an odd odor | 
| B. | malic acid may precipitate, leaving small crystals | 
| C. | the wine may be microbially unstable | 
| D. | the wine will be too low in acid | 
| Answer» D. the wine will be too low in acid | |
| 17. | Wort is | 
| A. | an aqueous extract of malt | 
| B. | malted barley | 
| C. | coagulated protein obtained during boiling | 
| D. | none of the above | 
| Answer» B. malted barley | |
| 18. | Which is not correct about blush wines? | 
| A. | They are stored in barrels | 
| B. | They are bottled within a year | 
| C. | They are usually slightly sweet | 
| D. | They are fermented in stainless steel | 
| Answer» B. They are bottled within a year | |
| 19. | Lagers are the beer in which fermentation is carried out using | 
| A. | top yeast | 
| B. | bottom yeast | 
| C. | either of these | 
| D. | middle yeast | 
| Answer» C. either of these | |
| 20. | Grist is | 
| A. | coarse powder obtained after milling of malted barley | 
| B. | an aqueous extract of malt | 
| C. | malted barley | 
| D. | coagulated protein obtained during boiling | 
| Answer» B. an aqueous extract of malt | |
| 21. | The term Racking refers to | 
| A. | stacking unlabeled wine in bins for aging | 
| B. | adding yeast to initiate fermentation | 
| C. | storing hoses to drain on a slanted board | 
| D. | removing clear liquid from sediment | 
| Answer» E. | |
| 22. | How long does it take to complete fermentation process in case of white wine? | 
| A. | 1-3 days | 
| B. | 3-5 days | 
| C. | 5-7 days | 
| D. | 7-11 days | 
| Answer» E. | |
| 23. | Which type of press produces the most high quality juice or wine? | 
| A. | Membrane press | 
| B. | Continuous press | 
| C. | Vertical basket press | 
| D. | Horizontal basket press | 
| Answer» B. Continuous press | |
| 24. | Carbonic maceration wines | 
| A. | are made from crushed grapes | 
| B. | are very fruity white wines | 
| C. | are produced in California | 
| D. | are low tannin red wines | 
| Answer» E. | |
| 25. | If a winemaker wanted to make a deeply colored red wine, which of the following should not be done? | 
| A. | Bleed off juice prior to fermenting on skins | 
| B. | Ferment at a high temperature | 
| C. | Use whole clusters | 
| D. | Punch down at least once a day | 
| Answer» D. Punch down at least once a day | |
| 26. | Alcohol content of sake varies from | 
| A. | 1-5 % | 
| B. | 4-17 % | 
| C. | 17-25% | 
| D. | more than 25% | 
| Answer» C. 17-25% | |
| 27. | Wine yeast | 
| A. | is sensitive to sulphur di oxide | 
| B. | is resistant to sulphur di oxide | 
| C. | shows enhanced enzyme production in presence of sulphur di oxide | 
| D. | none of the above | 
| Answer» C. shows enhanced enzyme production in presence of sulphur di oxide | |
| 28. | Final alcohol content in wine varies from | 
| A. | 6-9 % by weight | 
| B. | 8-13 % by weight | 
| C. | both (a) and (b) | 
| D. | 13-15 % by weight | 
| Answer» D. 13-15 % by weight | |
| 29. | Which of the following is true for the ale beer? | 
| A. | Top yeast of S cerevisiae is used | 
| B. | Fermentation is quick and completes in 5-7 days | 
| C. | Quantity of hops used is more than in lager beer | 
| D. | All of the above | 
| Answer» E. | |
| 30. | Bock beer is prepared from | 
| A. | roasted germinated barley seeds | 
| B. | rice | 
| C. | wheat | 
| D. | grapes | 
| Answer» B. rice | |
| 31. | Sherry is a type of | 
| A. | wine | 
| B. | beer | 
| C. | brandy | 
| D. | none of these | 
| Answer» B. beer | |
| 32. | What is the desirable sugar content of the grapes required for the wine production? | 
| A. | 2-5% | 
| B. | 5-10% | 
| C. | 10-14% | 
| D. | 14-20% | 
| Answer» E. | |
| 33. | The crushed grapes are known as | 
| A. | malt | 
| B. | must | 
| C. | wort | 
| D. | sonti | 
| Answer» C. wort | |
| 34. | The yeast generated during the fermentation of beer is generally separated by | 
| A. | centrifugation | 
| B. | filtration | 
| C. | cell disruption | 
| D. | all of these | 
| Answer» B. filtration | |
| 35. | Which is not the purpose of punching down? | 
| A. | To oxidize the wine | 
| B. | To extract tannin | 
| C. | To reduce possible microbial spoilage | 
| D. | To keep the cap moist | 
| Answer» B. To extract tannin | |
| 36. | The germination of barley kernels under controlled temperature and humidity to generate enzymes for the degradation of starch and protein is known as | 
| A. | brewing | 
| B. | malting | 
| C. | mashing | 
| D. | pitching | 
| Answer» C. mashing | |
| 37. | Which of the following organism is used for the fermentation of grapes? | 
| A. | <i>Rhizopus sonti</i> | 
| B. | <i>Aspergillus oryzae</i> | 
| C. | <i>Lactobacillus vermiformis</i> | 
| D. | <i>Saccharomyces cerevisiae</i> | 
| Answer» E. | |
| 38. | Malting process allows malt amylase and proteinases to degrade starch and protein to | 
| A. | glucose and peptone as well as peptides | 
| B. | glucose and amino acids | 
| C. | maltose and peptone as well as peptides | 
| D. | maltose and amino acids | 
| Answer» D. maltose and amino acids | |
| 39. | Ales are the beer in which fermentation is carried out using | 
| A. | top yeast | 
| B. | bottom yeast | 
| C. | either of the above | 
| D. | middle yeast | 
| Answer» B. bottom yeast | |
| 40. | The crystal-like material sometimes found in wine bottles is | 
| A. | residual diatomaceous earth | 
| B. | tannin | 
| C. | prevented by cold stabilizing | 
| D. | caused by excessive malic acid | 
| Answer» D. caused by excessive malic acid | |
| 41. | Wort is boiled with the hops | 
| A. | to help in protein coagulation | 
| B. | to provide bitter taste and flavour to the beer | 
| C. | to help in the coagulation of tannins | 
| D. | All of the above | 
| Answer» C. to help in the coagulation of tannins | |
| 42. | Sake is | 
| A. | rice beer or wine of India | 
| B. | wheat beer or wine | 
| C. | barley beer or wine | 
| D. | rice beer or wine of Japan | 
| Answer» E. | |
| 43. | Chaptalization is | 
| A. | adding sucrose to must | 
| B. | adding grape juice to must | 
| C. | adding sugar to wine before bottling | 
| D. | adding grape juice to wine before bottling | 
| Answer» B. adding grape juice to must | |
| 44. | The dried malt is the source of | 
| A. | amylase | 
| B. | proteinases | 
| C. | both (a) and (b) | 
| D. | cellulase | 
| Answer» D. cellulase | |
| 45. | In a hot climate, grapes lose acidity because | 
| A. | enough tartaric acid is not made | 
| B. | enough malic acid is not made | 
| C. | of break down of malic acid | 
| D. | of break down of tartaric acid | 
| Answer» D. of break down of tartaric acid | |
| 46. | In sparkling wine production, the cuvee is | 
| A. | the wire cage that holds the cork in place | 
| B. | the sugar addition just before corking | 
| C. | the sugar/yeast addition to the base wine | 
| D. | the base wine | 
| Answer» E. | |
| 47. | Volatile acidity is | 
| A. | also known as total acidity | 
| B. | produced by acetobacter | 
| C. | produced by Brettanomyces | 
| D. | known for producing a mousy or barnyard aroma | 
| Answer» C. produced by Brettanomyces | |
| 48. | How long does it take to complete fermentation process in case of red wine? | 
| A. | 1-3 days | 
| B. | 3-5 days | 
| C. | 5-7 days | 
| D. | 7-11 days | 
| Answer» C. 5-7 days | |