MCQOPTIONS
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MCQOPTIONS
This section includes 129 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 101. |
Which of the following statements is correct regarding S1 nuclease? |
| A. | It acts on double stranded DNA |
| B. | It acts on single stranded DNA |
| C. | It acts on both types of strands |
| D. | It is obtained from E. coli |
| Answer» C. It acts on both types of strands | |
| 102. |
Which of the following statements is correct with respect to exonuclease? |
| A. | They only act on single stranded DNA molecules |
| B. | They only act on double stranded DNA molecules |
| C. | They remove a single nucleotide base at a time |
| D. | They remove nucleotide bases from the middle of a polynucleotide chain |
| Answer» D. They remove nucleotide bases from the middle of a polynucleotide chain | |
| 103. |
What is the kinetics of the intramolecular and intermolecular ligation reactions? |
| A. | Second order kinetics for intramolecular and first order for intermolecular |
| B. | First order kinetics for intramolecular and second order for intermolecular |
| C. | Both are first order |
| D. | Both are second order |
| Answer» C. Both are first order | |
| 104. |
Template independent polymerases are the enzymes which add nucleotide bases without a template. Which of the following statements is correct with respect to these? |
| A. | They only add a single nucleotide |
| B. | They only add a string of nucleotides and not a single nucleotide |
| C. | Terminal transferase adds a series of nucleotides at the 3’ end |
| D. | Taq polymerase adds a single nucleotide at the 5’ end of the PCR product |
| Answer» D. Taq polymerase adds a single nucleotide at the 5’ end of the PCR product | |
| 105. |
If all the nucleotides are present with equal frequencies and at random, what are the chances of having a particular four nucleotide long motif? |
| A. | 1/256 |
| B. | 1/64 |
| C. | 1/16 |
| D. | 1/8 |
| Answer» B. 1/64 | |
| 106. |
A sequence is having two ends, 5’ and 3’. Which of the following statements is correct regarding the nature of the ends? |
| A. | The 5’ end is having hydroxyl group |
| B. | The 5’ end is having phosphate group |
| C. | The 3’ end is having phosphate group |
| D. | Any group can be present at any end |
| Answer» C. The 3’ end is having phosphate group | |
| 107. |
Removal of single stranded portions produced due to exonucleolytic activity and due to polymerase activity are termed as _____________ |
| A. | polishing and end filling respectively |
| B. | end filling and polishing respectively |
| C. | polishing in both the cases |
| D. | end filling in both the cases |
| Answer» B. end filling and polishing respectively | |
| 108. |
Electroporation is also used for taking up the DNA by the cells. It constitutes of ____________ |
| A. | inserting the DNA into the cells via an electric shock |
| B. | increased efficiency than both natural and chemical methods |
| C. | causing the least amount of damage in comparison to other methods |
| D. | decreased efficiency than both natural and chemical methods |
| Answer» B. increased efficiency than both natural and chemical methods | |
| 109. |
Why sticky ended ligations are carried out at temperatures lower than room temperature? |
| A. | It is so because the vibrational and kinetic energy of the molecules at room temperature is lower than that of the energy required to break the bonds holding the ends |
| B. | The energy required to break the bonds holding the ends is very less than that of the kinetic and vibrational energy at room temperature |
| C. | The enzyme carrying out ligation is unstable at low temperature |
| D. | The sticky ends created, don’t just relegate at low temperature |
| Answer» B. The energy required to break the bonds holding the ends is very less than that of the kinetic and vibrational energy at room temperature | |
| 110. |
The charge present on the DNA backbone is negative. The force required to accelerate the molecules towards anode is directly proportional to the number of ____________ |
| A. | sugar molecules |
| B. | nitrogenous bases |
| C. | phosphate groups |
| D. | both phosphate group and sugar molecules |
| Answer» D. both phosphate group and sugar molecules | |
| 111. |
What happens if a DNA molecule is treated by first Exonuclease III and then followed by treatment with S1 nuclease? |
| A. | The molecule is shortened only from 3’ end |
| B. | The molecule is shortened only from 5’ end |
| C. | The molecule is shortened from both the ends |
| D. | Only Exonuclease acts and S1 doesn’t acts |
| Answer» D. Only Exonuclease acts and S1 doesn’t acts | |
| 112. |
In the case of microarrays ____________ |
| A. | Often the whole organism is not sequenced |
| B. | Changes in transcript level can be studied in response to environmental changes |
| C. | RNA is synthesized only after the property which is to be studied is inculcated |
| D. | DNA is synthesized for this RNA by transcriptase |
| Answer» C. RNA is synthesized only after the property which is to be studied is inculcated | |
| 113. |
Which of the following steps is not included in the transferring of DNA? |
| A. | The gel containing the DNA is immersed in the buffer in the reservoir |
| B. | The buffer flows up through the gel via a wick |
| C. | Membrane is placed over the gel |
| D. | The nucleic acids are dissolved in the buffer and are obtained from it via pipetting |
| Answer» E. | |
| 114. |
In the case DNA is transferred from gel to the membrane, it is known as ____________ |
| A. | southern blot |
| B. | western blot |
| C. | northern blot |
| D. | south-western blot |
| Answer» B. western blot | |
| 115. |
How many classes of restriction enzymes are there? |
| A. | 2 |
| B. | 1 |
| C. | 3 |
| D. | 4 |
| Answer» D. 4 | |
| 116. |
UV shadowing is used at times for visualising the DNA. Which of the statements is correct for it? |
| A. | In this method, the DNA bands are visualized because they fluoresce under UV |
| B. | The DNA bands won’t fluoresce but will absorb the UV light |
| C. | It is not suitable for visualizing single stranded molecules |
| D. | It is less preferred over the use of ethidium bromide |
| Answer» C. It is not suitable for visualizing single stranded molecules | |
| 117. |
How many categories of ligation reaction are there on the basis of ends created? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 118. |
Isocaudomers are defined as the enzymes which recognize different sequence but generate same ends. Which of the following pairs of enzymes can be termed as isocuadomers? |
| A. | DpnI (GA|TC) and Sau3A (|GATC) |
| B. | BamHI (G|GATCC) and Sau3A (|GATC) |
| C. | DpnI (GA|TC) and BglII (A|GATCT) |
| D. | XbaI (T|CTAGA) and BamHI (G|GATCC) |
| Answer» C. DpnI (GA|TC) and BglII (A|GATCT) | |
| 119. |
The recognition sequence is at times palindromic in nature. Which of the following statements is correct with respect to it? |
| A. | The molecules which are cut by the same enzyme, anneal only if the sequence is palindromic in nature |
| B. | When the molecules are cleaved by the same enzyme and the recognition sequence is palindromic in nature, there is no effect on annealing |
| C. | There are increased chances of annealing if the recognition sequence is palindromic in nature |
| D. | The term ‘palindromic’ can be used whether the sequence is read from 5’ to 3’ or 3’ to 5’ |
| Answer» D. The term ‘palindromic’ can be used whether the sequence is read from 5’ to 3’ or 3’ to 5’ | |
| 120. |
If further electrophoresis is used for recovery of DNA from gels, the method is termed as ____________ |
| A. | secondary electrophoresis |
| B. | recovery electrophoresis |
| C. | denaturing electrophoresis |
| D. | electro-elution |
| Answer» E. | |
| 121. |
The RNA level ___________ the steady-state level of the corresponding protein directly and the post-translational modification of the protein ____________ |
| A. | reflects, can be determined |
| B. | reflects, can’t be determined |
| C. | doesn’t necessarily reflects, can be determined |
| D. | doesn’t necessarily reflects, can’t be determined |
| Answer» E. | |
| 122. |
Besides enzymatic means, physical stress can also be used for cleaving the DNA. Which of the following statements is true? |
| A. | Sonication, needles, syringes, etc all come under the category of physical stress |
| B. | In physical stress, there are no chances of contamination |
| C. | The ends obtained have unique sequences |
| D. | They are less effective than DNase treatment |
| Answer» B. In physical stress, there are no chances of contamination | |
| 123. |
What is the function of methylase? |
| A. | Addition of methyl groups to DNA |
| B. | Removal of methyl groups from DNA |
| C. | Both in removal and addition of methyl groups from DNA |
| D. | It is used in production of methane gas |
| Answer» B. Removal of methyl groups from DNA | |
| 124. |
Cell lysis is carried out by which substance? |
| A. | Lysozyme and detergents |
| B. | Water |
| C. | Sugar solution |
| D. | Suphuric Acid |
| Answer» B. Water | |
| 125. |
Purification of DNA by using silica derivatized groups by DEAE is termed as ____________ |
| A. | ion exchange resin based method |
| B. | silica based purification |
| C. | atom based resin exchange method |
| D. | packed bed purification |
| Answer» B. silica based purification | |
| 126. |
Nucleic acid precipitated constitutes of ____________ |
| A. | plasmid DNA |
| B. | plasmid DNA, along with RNA and chromosomal DNA |
| C. | rna alone |
| D. | chromosomal DNA only |
| Answer» C. rna alone | |
| 127. |
Which of the following components bind to the solid column made of silica, under high salt concentration? |
| A. | Proteins |
| B. | Polysaccharides |
| C. | Both proteins and polysaccharides |
| D. | Plasmid DNA |
| Answer» E. | |
| 128. |
Adsorption onto a solid phase support followed by elution is used as an alternative for separation of which component? |
| A. | chromosomal DNA |
| B. | plasmid DNA |
| C. | RNA alone |
| D. | other impurities |
| Answer» C. RNA alone | |
| 129. |
Treatment with exonuclease leads to removal of ____________ |
| A. | remnants of chromosomal DNA |
| B. | RNase |
| C. | plasmid DNA which is circularized |
| D. | proteins |
| Answer» B. RNase | |