MCQOPTIONS
Saved Bookmarks
MCQOPTIONS
This section includes 8 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Selection of molecules by lack of inserts on the basis of the sacB gene is known as: |
| A. | positive selection |
| B. | negative selection |
| C. | counter selection |
| D. | sacB selection |
| Answer» D. sacB selection | |
| 2. |
How many origins of replication are there once the cyclization is carried out by loxP sites? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 3. |
Circularization must take place in the bacterial cells before the replication starts. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 4. |
What happens after packaging is carried out in the phage head? |
| A. | A sequence dependent cleavage of DNA is carried out |
| B. | A sequence independent cleavage of DNA is carried out |
| C. | They are linearized before they are infected into the bacterial cells |
| D. | They are circularized before they are infected into the bacterial cells |
| Answer» C. They are linearized before they are infected into the bacterial cells | |
| 5. |
Which of the following is not included as a step in cloning in bacteriophage PI? |
| A. | The growth of vector is done as a plasmid by using pBR322 origin of replication |
| B. | Cleaving with BamH1 is done and then products cleaved with Sau3A are ligated |
| C. | Incubation with mutant lysogen is done which doesn t cleaves the recombinants at pac sites |
| D. | Incubation is done with mutant lysogen which doesn t contains head and tail proteins |
| Answer» D. Incubation is done with mutant lysogen which doesn t contains head and tail proteins | |
| 6. |
What is the function of the pac site in the bacteriophage PI? |
| A. | It is responsible for the initiation of packaging |
| B. | It is responsible for the termination of packaging |
| C. | It is responsible for the initiation of replication |
| D. | It is responsible for the termination of replication |
| Answer» B. It is responsible for the termination of packaging | |
| 7. |
If recombination takes place between elements in the second strain, it would replicate by the origin of replication of mini Mu. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 8. |
Choose the incorrect statement for Mini Mu ? |
| A. | It is produced by cloning of bacteriophage Mu in vivo |
| B. | It contains reduced phage genome |
| C. | It doesn t contain replication of origin of plasmid |
| D. | A selectable marker is added |
| Answer» D. A selectable marker is added | |